Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 741462..742237 Replicon chromosome
Accession NZ_CP088997
Organism Klebsiella pneumoniae strain 49088

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag LRZ82_RS03710 Protein ID WP_004150910.1
Coordinates 741752..742237 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag LRZ82_RS03705 Protein ID WP_004150912.1
Coordinates 741462..741755 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRZ82_RS03685 (LRZ82_03685) 736670..737272 - 603 WP_062954968.1 short chain dehydrogenase -
LRZ82_RS03690 (LRZ82_03690) 737370..738281 + 912 WP_004188550.1 LysR family transcriptional regulator -
LRZ82_RS03695 (LRZ82_03695) 738282..739430 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
LRZ82_RS03700 (LRZ82_03700) 739441..740817 - 1377 WP_004217775.1 cystathionine beta-synthase -
LRZ82_RS03705 (LRZ82_03705) 741462..741755 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
LRZ82_RS03710 (LRZ82_03710) 741752..742237 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
LRZ82_RS03715 (LRZ82_03715) 742941..743534 + 594 WP_202177850.1 hypothetical protein -
LRZ82_RS03720 (LRZ82_03720) 743631..743847 + 217 Protein_730 transposase -
LRZ82_RS03725 (LRZ82_03725) 744453..745325 + 873 WP_004188557.1 ParA family protein -
LRZ82_RS03730 (LRZ82_03730) 745325..745708 + 384 WP_004150906.1 hypothetical protein -
LRZ82_RS03735 (LRZ82_03735) 745701..747068 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 743631..743783 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T227289 WP_004150910.1 NZ_CP088997:741752-742237 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T227289 NZ_CP122827:34767-34919 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT227289 WP_004150912.1 NZ_CP088997:741462-741755 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT227289 NZ_CP122827:c34712-34655 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References