Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 741189..741964 Replicon chromosome
Accession NZ_CP072463
Organism Klebsiella pneumoniae strain KP1517

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag HRG83_RS03700 Protein ID WP_004150910.1
Coordinates 741479..741964 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag HRG83_RS03695 Protein ID WP_004150912.1
Coordinates 741189..741482 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HRG83_RS03675 (HRG83_03675) 736397..736999 - 603 WP_062954968.1 short chain dehydrogenase -
HRG83_RS03680 (HRG83_03680) 737097..738008 + 912 WP_004188550.1 LysR family transcriptional regulator -
HRG83_RS03685 (HRG83_03685) 738009..739157 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
HRG83_RS03690 (HRG83_03690) 739168..740544 - 1377 WP_004217775.1 cystathionine beta-synthase -
HRG83_RS03695 (HRG83_03695) 741189..741482 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
HRG83_RS03700 (HRG83_03700) 741479..741964 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
HRG83_RS03705 (HRG83_03705) 742668..743261 + 594 WP_004188553.1 hypothetical protein -
HRG83_RS03710 (HRG83_03710) 743358..743574 + 217 Protein_730 transposase -
HRG83_RS03715 (HRG83_03715) 744180..745052 + 873 WP_004188557.1 ParA family protein -
HRG83_RS03720 (HRG83_03720) 745052..745435 + 384 WP_004150906.1 hypothetical protein -
HRG83_RS03725 (HRG83_03725) 745428..746795 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 743358..743510 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T197835 WP_004150910.1 NZ_CP072463:741479-741964 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T197835 NZ_CP096764:450990-451379 [Acinetobacter baumannii]
TTGATCAACCATTTGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AGTTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCACAAATTGCTTATTTAGATAAAAAACTATGGTCGCTTGCTTATAAGTCACAGAACACCGTT
TCTCGCGTCAAAATTTTAAAAATAATTGATTGTCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCTATTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT197835 WP_004150912.1 NZ_CP072463:741189-741482 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT197835 NZ_CP096764:450726-450983 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ACCCTGATTTACTAGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References