Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 5184074..5184849 Replicon chromosome
Accession NZ_CP065438
Organism Klebsiella pneumoniae strain Mrs. Puff

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag I5M55_RS25430 Protein ID WP_004150910.1
Coordinates 5184074..5184559 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag I5M55_RS25435 Protein ID WP_004150912.1
Coordinates 5184556..5184849 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5M55_RS25405 5179243..5180610 - 1368 WP_004150905.1 replicative DNA helicase -
I5M55_RS25410 5180603..5180986 - 384 WP_004150906.1 hypothetical protein -
I5M55_RS25415 5180986..5181858 - 873 WP_004188557.1 ParA family protein -
I5M55_RS25420 5182464..5182680 - 217 Protein_4967 transposase -
I5M55_RS25425 5182777..5183370 - 594 WP_004188553.1 hypothetical protein -
I5M55_RS25430 5184074..5184559 - 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
I5M55_RS25435 5184556..5184849 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
I5M55_RS25440 5185494..5186870 + 1377 WP_004217775.1 cystathionine beta-synthase -
I5M55_RS25445 5186881..5188029 + 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
I5M55_RS25450 5188030..5188941 - 912 WP_004188550.1 LysR family transcriptional regulator -
I5M55_RS25455 5189039..5189641 + 603 WP_062954968.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - - 5125748..5185100 59352
flank IS/Tn - - 5182528..5182680 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T182653 WP_004150910.1 NZ_CP065438:c5184559-5184074 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T182653 NZ_CP086526:1369657-1370055 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT182653 WP_004150912.1 NZ_CP065438:c5184849-5184556 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT182653 NZ_CP086526:1369430-1369657 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References