Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 741965..742740 Replicon chromosome
Accession NZ_CP028797
Organism Klebsiella pneumoniae strain WCHKP040035

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag C6M51_RS05080 Protein ID WP_004150910.1
Coordinates 742255..742740 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag C6M51_RS05075 Protein ID WP_004150912.1
Coordinates 741965..742258 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6M51_RS05055 737173..737775 - 603 WP_062954968.1 short chain dehydrogenase -
C6M51_RS05060 737873..738784 + 912 WP_004188550.1 LysR family transcriptional regulator -
C6M51_RS05065 738785..739933 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
C6M51_RS05070 739944..741314 - 1371 WP_004150913.1 cystathionine beta-synthase -
C6M51_RS05075 741965..742258 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
C6M51_RS05080 742255..742740 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
C6M51_RS05085 743444..744037 + 594 WP_004188553.1 hypothetical protein -
C6M51_RS05090 744134..744350 + 217 Protein_759 IS3 family transposase -
C6M51_RS05095 744498..744683 + 186 WP_085666571.1 hypothetical protein -
C6M51_RS05100 744625..744846 + 222 WP_004188555.1 hypothetical protein -
C6M51_RS05105 744956..745828 + 873 WP_004188557.1 ParA family protein -
C6M51_RS05110 745828..746211 + 384 WP_004150906.1 hypothetical protein -
C6M51_RS05115 746204..747571 + 1368 WP_004150905.1 replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 744134..744286 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T104180 WP_004150910.1 NZ_CP028797:742255-742740 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T104180 NZ_CP028797:742255-742740 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT104180 WP_004150912.1 NZ_CP028797:741965-742258 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT104180 NZ_CP028797:741965-742258 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References