Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 718132..718907 Replicon chromosome
Accession NZ_CP083066
Organism Klebsiella pneumoniae strain 1613015451

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A331A5C5
Locus tag K9F39_RS03615 Protein ID WP_019704987.1
Coordinates 718422..718907 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag K9F39_RS03610 Protein ID WP_004150912.1
Coordinates 718132..718425 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9F39_RS03590 (713341) 713341..713943 - 603 WP_004174410.1 short chain dehydrogenase -
K9F39_RS03595 (714041) 714041..714952 + 912 WP_004181308.1 LysR family transcriptional regulator -
K9F39_RS03600 (714953) 714953..716101 - 1149 WP_094645045.1 PLP-dependent aspartate aminotransferase family protein -
K9F39_RS03605 (716112) 716112..717488 - 1377 WP_004217775.1 cystathionine beta-synthase -
K9F39_RS03610 (718132) 718132..718425 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
K9F39_RS03615 (718422) 718422..718907 + 486 WP_019704987.1 GNAT family N-acetyltransferase Toxin
K9F39_RS03620 (719605) 719605..720198 + 594 WP_004188553.1 hypothetical protein -
K9F39_RS03625 (720295) 720295..720511 + 217 Protein_711 transposase -
K9F39_RS03635 (721515) 721515..722228 - 714 WP_002916694.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 720295..720447 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17572.56 Da        Isoelectric Point: 8.5111

>T216084 WP_019704987.1 NZ_CP083066:718422-718907 [Klebsiella pneumoniae]
MISAPEPLNAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T216084 NZ_CP112862:222311-222577 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAAACGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTAGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT216084 WP_004150912.1 NZ_CP083066:718132-718425 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT216084 NZ_CP112862:222048-222311 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTGGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTAGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A331A5C5

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References