Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 741849..742624 Replicon chromosome
Accession NZ_CP101542
Organism Klebsiella pneumoniae subsp. pneumoniae strain KP21

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag NMY29_RS03705 Protein ID WP_004150910.1
Coordinates 742139..742624 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag NMY29_RS03700 Protein ID WP_004150912.1
Coordinates 741849..742142 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NMY29_RS03680 (NMY29_03680) 737057..737659 - 603 WP_062954968.1 short chain dehydrogenase -
NMY29_RS03685 (NMY29_03685) 737757..738668 + 912 WP_004188550.1 LysR family transcriptional regulator -
NMY29_RS03690 (NMY29_03690) 738669..739817 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
NMY29_RS03695 (NMY29_03695) 739828..741204 - 1377 WP_004217775.1 cystathionine beta-synthase -
NMY29_RS03700 (NMY29_03700) 741849..742142 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
NMY29_RS03705 (NMY29_03705) 742139..742624 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
NMY29_RS03710 (NMY29_03710) 743328..743921 + 594 WP_004188553.1 hypothetical protein -
NMY29_RS03715 (NMY29_03715) 744018..744234 + 217 Protein_731 transposase -
NMY29_RS03720 (NMY29_03720) 744840..745712 + 873 WP_004188557.1 ParA family protein -
NMY29_RS03725 (NMY29_03725) 745712..746095 + 384 WP_004150906.1 hypothetical protein -
NMY29_RS03730 (NMY29_03730) 746088..747455 + 1368 WP_004150905.1 SPI-7-type island replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 744018..744170 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T252239 WP_004150910.1 NZ_CP101542:742139-742624 [Klebsiella pneumoniae subsp. pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT252239 WP_004150912.1 NZ_CP101542:741849-742142 [Klebsiella pneumoniae subsp. pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References