63508

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2818340..2819115 Replicon chromosome
Accession NZ_CP015822
Organism Klebsiella pneumoniae isolate blood sample

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag A8N26_RS14305 Protein ID WP_004150910.1
Coordinates 2818340..2818825 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag A8N26_RS14310 Protein ID WP_004150912.1
Coordinates 2818822..2819115 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A8N26_RS14280 2813509..2814876 - 1368 WP_004150905.1 replicative DNA helicase -
A8N26_RS14285 2814869..2815252 - 384 WP_004150906.1 hypothetical protein -
A8N26_RS14290 2815252..2816124 - 873 WP_004188557.1 ParA family protein -
A8N26_RS14295 2816730..2816946 - 217 Protein_2777 transposase -
A8N26_RS14300 2817043..2817636 - 594 WP_004188553.1 hypothetical protein -
A8N26_RS14305 2818340..2818825 - 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
A8N26_RS14310 2818822..2819115 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
A8N26_RS14315 2819760..2821136 + 1377 WP_004217775.1 cystathionine beta-synthase -
A8N26_RS14320 2821147..2822295 + 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
A8N26_RS14325 2822296..2823207 - 912 WP_004188550.1 LysR family transcriptional regulator -
A8N26_RS14330 2823305..2823907 + 603 WP_002916735.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - - 2761319..2819366 58047
flank IS/Tn - - 2816794..2816946 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T63508 WP_004150910.1 NZ_CP015822:c2818825-2818340 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T63508 NZ_CP015822:c2818825-2818340 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT63508 WP_004150912.1 NZ_CP015822:c2819115-2818822 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT63508 NZ_CP015822:c2819115-2818822 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References