Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 736808..737583 Replicon chromosome
Accession NZ_CP066528
Organism Klebsiella pneumoniae strain WCHKP090361

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag H3G66_RS03680 Protein ID WP_004150910.1
Coordinates 737098..737583 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag H3G66_RS03675 Protein ID WP_004150912.1
Coordinates 736808..737101 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H3G66_RS03655 732016..732618 - 603 WP_062954968.1 short chain dehydrogenase -
H3G66_RS03660 732716..733627 + 912 WP_004188550.1 LysR family transcriptional regulator -
H3G66_RS03665 733628..734776 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
H3G66_RS03670 734787..736163 - 1377 WP_004217775.1 cystathionine beta-synthase -
H3G66_RS03675 736808..737101 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
H3G66_RS03680 737098..737583 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
H3G66_RS03685 738287..738880 + 594 WP_004188553.1 hypothetical protein -
H3G66_RS03690 738977..739193 + 217 Protein_725 transposase -
H3G66_RS03695 739799..740671 + 873 WP_004188557.1 ParA family protein -
H3G66_RS03700 740671..741054 + 384 WP_004150906.1 hypothetical protein -
H3G66_RS03705 741047..742414 + 1368 WP_004150905.1 replicative DNA helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 738977..739129 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T184561 WP_004150910.1 NZ_CP066528:737098-737583 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T184561 NZ_CP087686:2274966-2275244 [Xylella taiwanensis]
ATGATTAAAACCTTTGCCGACCAGGACACGGAATCTTTGTTCAACGGGTCACGGGTACGTCGCTTTATTCACATAGAAGC
GGTCGCACGCCGGAAGCTGATTCAATTGCATGCAGCAGCGACCTTGAGCTTTCTCAATGCGCCGCCTGGTAATCGGCTTG
AAACCTTAAAAGGGGACCGCCAAGGGCAGCACAGCATCCGTATCAACAATCAATTCCGCCTCTGTTTCATTTGGGAAAAC
GGCGATGCCTATCAGGTTGAAATTGTCGATTATCACTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT184561 WP_004150912.1 NZ_CP066528:736808-737101 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT184561 NZ_CP087686:2275248-2275547 [Xylella taiwanensis]
ATGAGCATGTCCAAGACACTTCCCCCCATTCACCCAGGAGAAATTCTCCGGGAAGAATTTATGGCCCCTCTAGGCTTATC
CAGCAATGCGCTGGCCAAGGCGATTGGCGTGACGGCGGCACGGATCAACGAGATTGTGCGCGAACGACGTGGGATCACGG
CGGATACTGCACTGCGTCTGGCACGTTACTTTGGCACCGATCCGCAAAGCTGGCTGAATCTGGAACAGCACTATGCACTC
GAATGCGCCAAACGCGACCTTGGAGAGGCCCTGTCACGTATTCACCCTCGCGTCGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References