Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 691574..692349 Replicon chromosome
Accession NZ_CP073783
Organism Klebsiella pneumoniae isolate KP_NORM_BLD_2014_96855

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A331A5C5
Locus tag KFO63_RS03440 Protein ID WP_019704987.1
Coordinates 691864..692349 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag KFO63_RS03435 Protein ID WP_004150912.1
Coordinates 691574..691867 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFO63_RS03415 (KFO63_03415) 686783..687385 - 603 WP_004174410.1 short chain dehydrogenase -
KFO63_RS03420 (KFO63_03420) 687483..688394 + 912 WP_069339825.1 LysR family transcriptional regulator -
KFO63_RS03425 (KFO63_03425) 688395..689543 - 1149 WP_087796222.1 PLP-dependent aspartate aminotransferase family protein -
KFO63_RS03430 (KFO63_03430) 689554..690930 - 1377 WP_004181304.1 cystathionine beta-synthase -
KFO63_RS03435 (KFO63_03435) 691574..691867 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
KFO63_RS03440 (KFO63_03440) 691864..692349 + 486 WP_019704987.1 GNAT family N-acetyltransferase Toxin
KFO63_RS03445 (KFO63_03445) 693053..693646 + 594 WP_004188553.1 hypothetical protein -
KFO63_RS03450 (KFO63_03450) 693743..693959 + 217 Protein_678 transposase -
KFO63_RS03460 (KFO63_03460) 694827..695540 - 714 WP_002916694.1 DUF554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 693743..693895 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17572.56 Da        Isoelectric Point: 8.5111

>T200248 WP_019704987.1 NZ_CP073783:691864-692349 [Klebsiella pneumoniae]
MISAPEPLNAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T200248 NZ_CP097726:4204375-4204632 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT200248 WP_004150912.1 NZ_CP073783:691574-691867 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT200248 NZ_CP097726:4204644-4205189 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A331A5C5

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References