Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1854124..1854899 Replicon chromosome
Accession NZ_CP063890
Organism Klebsiella pneumoniae strain BS433-2

Toxin (Protein)


Gene name TacT2 Uniprot ID B6ZBI6
Locus tag ISB10_RS08955 Protein ID WP_004889762.1
Coordinates 1854124..1854609 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag ISB10_RS08960 Protein ID WP_004150912.1
Coordinates 1854606..1854899 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISB10_RS08935 1851123..1851836 + 714 WP_002916694.1 DUF554 domain-containing protein -
ISB10_RS08945 1852514..1852730 - 217 Protein_1745 transposase -
ISB10_RS08950 1852827..1853420 - 594 WP_004188553.1 hypothetical protein -
ISB10_RS08955 1854124..1854609 - 486 WP_004889762.1 GNAT family N-acetyltransferase Toxin
ISB10_RS08960 1854606..1854899 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
ISB10_RS08965 1855543..1856919 + 1377 WP_014906931.1 pyridoxal-phosphate dependent enzyme -
ISB10_RS08970 1856930..1858078 + 1149 WP_014906930.1 PLP-dependent aspartate aminotransferase family protein -
ISB10_RS08975 1858079..1858990 - 912 WP_014906929.1 LysR family transcriptional regulator -
ISB10_RS08980 1859088..1859690 + 603 WP_002916735.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 1852578..1852730 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17585.56 Da        Isoelectric Point: 8.5144

>T179244 WP_004889762.1 NZ_CP063890:c1854609-1854124 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDSMMLMVTLRDLVN
A

Download         Length: 486 bp

>T179244 NZ_CP084654:3958361-3958540 [Pectobacterium versatile]
ATGGTCATTCCGCTTATCGAAGCACATCACCTCTCTGAGAAAGTGAATAAGACGTTGTTCCCTCTACTCCGCATCTACGG
TGAAGATTATCGGTTAATGACCACTGAGCTATCGAGTGTCCTCGATGAGGTTATTGGTGAGGCTGTAAGTATCCCGGTAA
ACCGAAGCATTCACTTTTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT179244 WP_004150912.1 NZ_CP063890:c1854899-1854606 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT179244 NZ_CP084654:3958072-3958278 [Pectobacterium versatile]
ATGAAACACCGGATTAGCATTGCCGTGGATAAAGACGATAATCAGAGCCCCAATTCTGCCGGGATACCTATTTCTGGTCT
GGTGAATGACGAAGCCAACCGCATCAAAACTGAGGTGTGGAAGAAAGAAAACCGCGAAGGGATGGAAAACGTCGCCAAAT
TTATCGCACAGAATAGCTCCTTTGCGGATGAAAATAGGAACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A447W563

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References