Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2732860..2733635 Replicon chromosome
Accession NZ_CP009114
Organism Klebsiella pneumoniae strain carbapenem-resistant blaNDM-1

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag FH42_RS13760 Protein ID WP_004150910.1
Coordinates 2732860..2733345 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag FH42_RS13765 Protein ID WP_004150912.1
Coordinates 2733342..2733635 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FH42_RS13735 2728029..2729396 - 1368 WP_004150905.1 replicative DNA helicase -
FH42_RS13740 2729389..2729772 - 384 WP_004150906.1 hypothetical protein -
FH42_RS13745 2729772..2730644 - 873 WP_004188557.1 ParA family protein -
FH42_RS13750 2731250..2731466 - 217 Protein_2663 transposase -
FH42_RS13755 2731563..2732156 - 594 WP_038430882.1 hypothetical protein -
FH42_RS13760 2732860..2733345 - 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
FH42_RS13765 2733342..2733635 - 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
FH42_RS13770 2734280..2735656 + 1377 WP_004217775.1 cystathionine beta-synthase -
FH42_RS13775 2735667..2736815 + 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
FH42_RS13780 2736816..2737727 - 912 WP_004188550.1 LysR family transcriptional regulator -
FH42_RS13785 2737825..2738427 + 603 WP_002916735.1 short chain dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - - 2675897..2733886 57989
flank IS/Tn - - 2731314..2731466 152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T48415 WP_004150910.1 NZ_CP009114:c2733345-2732860 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T48415 NZ_CP009114:c2733345-2732860 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT48415 WP_004150912.1 NZ_CP009114:c2733635-2733342 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT48415 NZ_CP009114:c2733635-2733342 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References