SecReT6, T6SS database

Enterobacteriaceae

Experimental investigation has been performed.

Bioinformatics investigation has been performed

T6SS ID	Type	Organism	Replicon (Accession)	Location	Function
T6SS00463	i2	Escherichia coli 042	chromosome (NC_017626)	4852875..4885437	effector translocation
T6SS00464	i4b	Escherichia coli 042	chromosome (NC_017626)	4893076..4921378	effector translocation
T6SS00396	i2	Enterobacter cloacae subsp. cloacae ATCC 13047	chromosome (NC_014121)	1819466..1844686	anti-bacterial; anti-eukaryotic
T6SS00344	i2	Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044	chromosome (NC_012731)	2298791..2328477	anti-bacterial; anti-eukaryotic
T6SS00345	Unknown	Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044	chromosome (NC_012731)	3190436..3214872	anti-bacterial; anti-eukaryotic
T6SS00023	i3	Salmonella enterica subsp. enterica serovar Typhimurium str. LT2	chromosome (NC_003197)	304665..339275	anti-bacterial
T6SS00303	i1	Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91	chromosome (NC_011274)	1114451..1137704	anti-eukaryotic
T6SS00535	i3	Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344	chromosome (NC_016810)	304656..332038	anti-eukaryotic; virulence
T6SS00301	i3	Salmonella enterica subsp. enterica serovar Dublin str. CT_02021853	chromosome (NC_011205)	305501..331344	anti-eukaryotic; virulence
T6SS00551	i3	Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S	chromosome (NC_016856)	305365..332747	anti-eukaryotic; virulence
T6SS00037	i2	Escherichia coli CFT073	chromosome (NC_004431)	3217751..3253963	anti-eukaryotic; virulence
T6SS00905	i1	Escherichia coli strain TW-XM	chromosome (KF678350)	1..25901	anti-eukaryotic; effector translocation
T6SS00904	i2	Escherichia coli strain TW-XM	chromosome (KF678349)	1..29754	anti-eukaryotic; effector translocation
T6SS00787	i1	Escherichia coli RS218	chromosome (JN837480)	1..23615	anti-eukaryotic; effector translocation; adherence
T6SS00789	i3	Salmonella enterica GIFU10007	chromosome (JF766712)	1..38426	anti-eukaryotic; effector translocation; virulence
T6SS00896	i3	Salmonella enterica subsp. enterica serovar Typhimurium	chromosome (AJ320483)	322..27706	anti-eukaryotic
T6SS00395	i3	Enterobacter cloacae subsp. cloacae ATCC 13047	chromosome (NC_014121)	1551980..1588894	anti-bacterial; anti-eukaryotic; effector translocation
T6SS00930	i1	Escherichia coli SEPT362	chromosome (NZ_AOGL01000004)	11200..34631	anti-eukaryotic; adherence
T6SS00931	i4b	Escherichia coli 17-2	chromosome (NZ_JACEFV010000001)	883479..901846	anti-eukaryotic; effector translocation
T6SS00935	i2	Escherichia coli 17-2	chromosome (NZ_JACEFV010000001)	909481..942047	anti-eukaryotic; effector translocation
T6SS00937	i1	Escherichia coli DE719	chromosome (KM251715)	1..24039	anti-eukaryotic
T6SS00941	Unknown	Escherichia coli strain STEC004	chromosome I (KX118288)	1..7947	anti-bacterial
T6SS00942	Unknown	Escherichia coli strain PE027	chromosome I (KX118287)	1..7577	anti-bacterial
T6SS00943	Unknown	Escherichia coli strain PE321	chromosome I (KX118290)	81..2733	anti-bacterial
T6SS00944	Unknown	Escherichia coli strain PE086	chromosome I (KX118289)	81..3542	anti-bacterial
T6SS00946	Unknown	Escherichia coli strain PE117	chromosome I (KX118293)	1..8366	anti-bacterial
T6SS00947	Unknown	Escherichia coli strain PE050	chromosome I (KX118292)	1..4946	anti-bacterial
T6SS01013	i3	Citrobacter rodentium DBS100	chromosome (CP038008)	2124567..2151602	-
T6SS01034	i1	Escherichia coli PCN033	chromosome (NZ_CP006632)	246100..267921	anti-bacterial; anti-eukaryotic; anti-adverse environment
T6SS01200	i2	Escherichia coli KTE165	chromosome (NZ_KB733141)	180921..202372	-
T6SS01210	Unknown	Escherichia coli strain O157 zh165	chromosome (KX118286)	1..6820	anti-bacterial
T6SS01214	Unknown	Klebsiella pneumoniae strain Kp52.145	chromosome (NZ_FO834906)	950009..972445	anti-bacterial; anti-eukaryotic
T6SS01215	i2	Klebsiella pneumoniae strain Kp52.145	chromosome (NZ_FO834906)	2564540..2589406	anti-bacterial; anti-eukaryotic
T6SS01216	Unknown	Klebsiella pneumoniae strain Kp52.145	chromosome (NZ_FO834906)	3477664..3505840	anti-bacterial; anti-eukaryotic
T6SS01273	Unknown	Klebsiella pneumoniae subsp. pneumoniae ATCC 43816	chromosome (NZ_CP064352)	850912..879397	anti-bacterial
T6SS01274	i2	Klebsiella pneumoniae subsp. pneumoniae ATCC 43816	chromosome (NZ_CP064352)	1759057..1784245	-
T6SS01282	i3	Kosakonia oryzae strain KO348	chromosome (NZ_JZLI01000006)	149587..193032	anti-bacterial; anti-adverse environment
T6SS01283	i2	Kosakonia oryzae strain KO348	chromosome (NZ_JZLI01000015)	107454..120808	anti-bacterial; anti-adverse environment
T6SS01291	i3	Salmonella enterica subsp. enterica serovar Typhimurium strain ATCC 14028	chromosome (NZ_MTFW01000011)	10531..37913	-
T6SS00548	i2	Klebsiella pneumoniae subsp. pneumoniae HS11286	chromosome (NC_016845)	2326237..2353260	anti-bacterial
T6SS13923	i3	Enterobacter sp. DSM 30060	chromosome (NZ_CP056118)	4465150..4496796	anti-bacterial
T6SS14097	i3	Enterobacter cloacae strain DSM 30054	chromosome (NZ_CP056776)	4037435..4081387	anti-bacterial
T6SS14098	i2	Enterobacter cloacae strain DSM 30054	chromosome (NZ_CP056776)	4297267..4345922	-
T6SS01249	Unknown	Shigella sonnei CIP106347	chromosome (-)	-	anti-bacterial
T6SS01256	Unknown	Cronobacter sakazakii ATCC 12868	chromosome (-)	-	anti-bacterial
T6SS01263	Unknown	Salmonella enterica subsp. enterica serovar Typhimurium isolate SAT52	chromosome (-)	-	anti-eukaryotic
T6SS17502	Unknown	Citrobacter freundii strain CF74	chromosome (-)	-	anti-eukaryotic

Experimental investigation has been performed.

Bioinformatics investigation has been performed

Regulator ID	Locus tag (Gene)	Related T6SS	Regulator type	Regulatory pathway	Organism	Replicon (Accession)	Coordinates (Strand)	NCBI ID	Target (Regulation)
REG0071	Z4049 (RpoS)	T6SS00006	protein	Global regulator	Escherichia coli O157:H7 str. EDL933	chromosome (NC_002655)	3654380..3655372 (-)	446003635	Z1921 (KatN) (↓)
REG0072	Z5519 (OxyR)	T6SS00006	protein	Global regulator	Escherichia coli O157:H7 str. EDL933	chromosome (NC_002655)	5033893..5034810 (+)	446948683	Z1921 (KatN) (↓)
REG0073	Z2013 (hns)	T6SS00006	protein	Global regulator	Escherichia coli O157:H7 str. EDL933	chromosome (NC_002655)	1829490..1829903 (-)	447210122	Z0264 (hcp-2) (↑)
REG0087	STM1751 (H-NS)	T6SS00023	protein	Global regulator	Salmonella enterica subsp. enterica serovar Typhimurium str. LT2	chromosome (NC_003197)	1847277..1847690 (+)	16765095	STM0272 (ClpV) promotor (↑); STM0279 (hcp) promotor (↑); STM0285 promotor (↑); STM0289 (VgrG) promotor (↑); STM0268 promotor (↑)
REG0088	STM0693 (Fur)	T6SS00023	protein	Global regulator	Salmonella enterica subsp. enterica serovar Typhimurium str. LT2	chromosome (NC_003197)	756397..756849 (-)	16764063	STM0272 (ClpV) promoter (↑)
REG0171	APECO1_487 (FNR)	T6SS00155	protein	Global regulator	Escherichia coli APEC O1	chromosome (NC_008563)	1455411..1456163 (-)	446534565	APECO1_1756 (VgrG1) (↓)
REG0203	KP1_3314 (hns)	T6SS00344	protein	Global regulator	Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044	chromosome (NC_012731)	3140175..3140582 (+)	238895262	KP1_2392 (TssB) promotor (↑); KP1_2392 (hcp) (↑)
REG0248	EC042_RS26480 (aggR)	T6SS00464	protein	Transcription regulator	Escherichia coli 042	plasmid (NC_017627)	41080..41877 (-)	446692110	EC042_RS26480 (aggR) promoter (↓); EC042_RS26480 (aggR) promoter (↓)
REG0249	EC042_RS18855 (FIS)	T6SS00464	protein	Transcription regulator	Escherichia coli 042	chromosome (NC_017626)	3772795..3773091 (+)	446385050	EC042_RS26480 (aggR) promoter (↓)
REG0250	EC042_RS06800 (Hns)	T6SS00464	protein	Global regulator	Escherichia coli 042	chromosome (NC_017626)	1376831..1377244 (-)	447210122	EC042_RS26480 (aggR) promoter (↑)
REG0251	EC042_RS03685 (Fur)	T6SS00464	protein	Global regulator	Escherichia coli 042	chromosome (NC_017626)	786918..787364 (-)	446053847	EC042_4532 promoter (↑); EC042_4524 (TssB) promotor (↑)
REG0252	EC042_RS19435 (Dam)	T6SS00464	protein	Global regulator	Escherichia coli 042	chromosome (NC_017626)	3861171..3862007 (-)	446664793	EC042_4532 promoter (↑)
REG0270	CXG97_03740 (Fur)	T6SS00904	protein	Transcription regulator	Escherichia coli strain ExPEC XM	chromosome (NZ_CP025328)	729474..729920 (-)	446053847	T6SS1 (TssB) promotor (↑)
REG0271	H1D38_08255 (fur)	T6SS00931	protein	Global regulator	Escherichia coli strain 17-2	chromosome (JACEFV010000001)	1744748..1745194 (-)	MBA2233871.1	sciH promotor (↑)
REG0272	H1D38_12840 (Dam)	T6SS00931	protein	Global regulator	Escherichia coli strain 17-2	chromosome (JACEFV010000002)	105877..106713 (+)	MBA2234741.1	sciH promotor (↑)
REG0273	W817_21870 (CpxR)	T6SS00937	protein	TCS-RR	Escherichia coli RS218	chromosome (NZ_CP007149)	4360812..4361510 (-)	446956464	hcp2B promoter (↓)
REG0274	W817_01610 (NplE)	T6SS00937	protein	TCS regulatory pathway	Escherichia coli RS218	chromosome (NZ_CP007149)	214947..215657 (+)	446161331	W817_RS21690 (CpxA) (↓)
REG0336	BWP10_01550 (ssrB)	T6SS01291	protein	TCS-RR	Salmonella enterica subsp. enterica serovar Typhimurium strain ATCC 14028	chromosome (MTFW01000001)	296600..299362 (+)	1145677935	sciS/TssM transcription (↑)
REG0338	BN49_RS12735 (phoQ)	T6SS01214	protein	TCS-RR	Klebsiella pneumoniae strain Kp52.145	chromosome (NZ_FO834906)	2324171..2325637 (-)	490249900	TssB transcription (↓)
REG0339	BN49_RS12735 (phoP)	T6SS01214	protein	TCS-HK	Klebsiella pneumoniae strain Kp52.145	chromosome (NZ_FO834906)	2325637..2326308 (-)	490252783	BN49_2224 (phoQ) (↓)
REG0340	BN49_RS29275 (mgrB)	T6SS01214	protein	TCS regulatory pathway	Klebsiella pneumoniae strain Kp52.145	chromosome (NZ_FO834906)	3583038..3583181 (-)	489000713	phoPQ (↑)
REG0341	BN49_RS21260 (rcsB)	T6SS01214	protein	TCS-RR	Klebsiella pneumoniae strain Kp52.145	chromosome (NZ_FO834906)	4066268..4066918 (+)	489002368	phoPQ (↑)
REG0342	BN49_RS18480 (h-ns)	T6SS01214	protein	Global regulator	Klebsiella pneumoniae strain Kp52.145	chromosome (NZ_FO834906)	3427636..3428043 (+)	488999426	TssB transcription (↑)
REG0343	BN49_RS10300 (fur)	T6SS01214	protein	Global regulator	Klebsiella pneumoniae strain Kp52.145	chromosome (NZ_FO834906)	1826878..1827330 (-)	514077307	TssB transcription (↓)
REG0344	BN49_RS26620 (hfq)	T6SS01214	protein	QS regulatory pathway	Klebsiella pneumoniae strain Kp52.145	chromosome (NZ_FO834906)	5173711..5174019 (-)	488974767	TssB transcription (↓)
REG0345	BN49_RS03755 (rpoN)	T6SS01214	protein	Global regulator	Klebsiella pneumoniae strain Kp52.145	chromosome (NZ_FO834906)	533564..534997 (-)	489007850	TssB transcription (↓)
REG0346	BN49_RS06445 (rpoS)	T6SS01214	protein	Global regulator	Klebsiella pneumoniae strain Kp52.145	chromosome (NZ_FO834906)	1060243..1061235 (+)	489004494	TssB transcription (↓)
REG0357	PPECC33_RS11180 (BaeS)	T6SS01034	protein	TCS-HK	Escherichia coli PCN033	chromosome (NZ_CP006632)	2271464..2272867 (+)	446597798	PPECC33_RS11185 (BaeR) (↓)
REG0358	PPECC33_RS11185 (BaeR)	T6SS01034	protein	TCS-RR	Escherichia coli PCN033	chromosome (NZ_CP006632)	2272864..2273586 (+)	446060022	T6SS promotor (↓)
REG0362	PPECC33_RS11945 (rcsB)	T6SS01034	protein	TCS-RR	Escherichia coli PCN033	chromosome (NZ_CP006632)	2429320..2429970 (+)	446984661	T6SS transcription (↓)
REG0347	sdiA	-	protein	Global regulator	Enterobacter cloacae strain JLD400	chromosome (-)	-	-	ENC_07270 transcript (↓)

Experimental investigation has been performed.

Bioinformatics investigation has been performed

Effector ID	Locus tag (Gene)	Classification	Cognate immunity protein	Related T6SS	Organism	Replicon (Accession)	Coordinates (Strand)	NCBI ID
EFF01453	Z1921	Tse	-	T6SS00006	Escherichia coli O157:H7 str. EDL933	chromosome (NC_002655)	1744916..1745806 (-)	15801381
EFF01418	ECL_01542 (Tae4)	Tae	IMU00269	T6SS00395	Enterobacter cloacae subsp. cloacae ATCC 13047	chromosome (NC_014121)	1563472..1563963 (+)	502861206
EFF01370	SL1344_RS01405	Tae	IMU00303	T6SS00535	Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344	chromosome (NC_016810)	317659..318144 (+)	378698249
EFF01380	STM0277 (Tae4)	Tae		T6SS00553	Salmonella enterica subsp. enterica serovar Typhimurium str. T000240	chromosome (NC_016860)	317684..318169 (+)	378698249
EFF01527	vt6B (VT1)	Tae	IMU00328	T6SS00947	Escherichia coli strain PE050	chromosome (KX118292)	2514..3167 (+)	1062685203
EFF01452	Z5014 (Rhs-CT5)	Tde	IMU00343	T6SS00006	Escherichia coli O157:H7 str. EDL933	chromosome (NC_002655)	4572113..4576246 (+)	15804134
EFF01467	SARI_02603	Tde	-	T6SS00237	Salmonella enterica subsp. arizonae serovar 62:z4z23:-	chromosome (NC_010067)	2520159..2523749 (-)	160865837
EFF01520	AAF13_24950 (Hcp-ET1)	Tde	IMU00344	-	Escherichia coli strain STEC004	chromosome (NZ_CP011331)	4965377..4966555 (-)	445988041
EFF01519	ECO111_0594 (Rhs-CT3)	Tde	IMU00342	-	Escherichia coli strain PE027	chromosome (NC_013364)	632578..637377 (-)	447211780
EFF01522	het1 (Hcp-ET3)	Tde	IMU00322 ; IMU00323 ; IMU00324	T6SS00944	Escherichia coli strain PE086	chromosome (KX118289)	81..1430 (+)	1062685123
EFF01523	het2 (Hcp-ET4)	Tde	-	T6SS00944	Escherichia coli strain PE086	chromosome (KX118289)	1508..1858 (+)	1062685124
EFF01810	STM14_0336 (Tlde1)	Tde	-	T6SS00551	Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S	chromosome (NC_016856)	329640..330161 (+)	446891128
EFF01525	vt4B (VT5)	Tge	IMU00329	T6SS00946	Escherichia coli strain PE117	chromosome (KX118293)	2536..3138 (+)	1062685230
EFF01348	xmoI (Tle4)	Tle	IMU00254	T6SS00904	Escherichia coli strain TW-XM	chromosome (KF678349)	11678..13447 (+)	611963687
EFF01465	EC042_RS24220	Tle	IMU00313	T6SS00931	Escherichia coli 042	chromosome (NC_017626)	4865605..4867287 (+)	284924255
EFF01521	A31K_02346 (Hcp-ET2)	Tle	IMU00345	T6SS01200	Escherichia coli KTE165	chromosome (NZ_KB733141)	2548806..2550806 (+)	431425568
EFF01872	ECL_01553 (Tle)	Tle	IMU00375	T6SS00395	Enterobacter cloacae subsp. cloacae ATCC 13047	chromosome (NC_014121)	1576408..1577826 (+)	502861217
EFF01498	-	Tpe	IMU00327	T6SS00931	Escherichia coli strain TW-XM	chromosome (KF678350)	24197..25636 (+)	611963723
EFF01518	ct1C (Rhs-CT1)	Tpe	IMU00321	T6SS00941	Escherichia coli strain STEC004	chromosome (KX118288)	2411..7273 (+)	1062685107
EFF01254	ECL_01567 (RhsA)	Tse	IMU00055	T6SS00395	Enterobacter cloacae subsp. cloacae ATCC 13047	chromosome (NC_014121)	1591543..1595931 (+)	296101929
EFF01255	ECL_03140 (RhsB)	Tse	IMU00056	T6SS00395	Enterobacter cloacae subsp. cloacae ATCC 13047	chromosome (NC_014121)	3200343..3204599 (-)	296103479
EFF01526	vt4B (VT5)	Tse	-	T6SS00946	Escherichia coli strain PE117	chromosome (KX118293)	3140..3691 (+)	1062685231
EFF01531	vt6C (VTI1)	Tse	-	T6SS00947	Escherichia coli strain PE050	chromosome (KX118292)	3177..3626 (+)	1062685204
EFF01826	BN49_RS18800 (VgrG4)	Tse	IMU00349	T6SS01216	Klebsiella pneumoniae strain Kp52.145	chromosome (NZ_FO834906)	3498991..3501690 (-)	802146982
EFF00020	Z0266 (Hcp2)	-	-	T6SS00006	Escherichia coli O157:H7 str. EDL933	chromosome (NC_002655)	264134..264652 (+)	447065702
EFF00505	ECL_01541 (hcp)	-	-	T6SS00395	Enterobacter cloacae subsp. cloacae ATCC 13047	chromosome (NC_014121)	1562931..1563413 (+)	495778061
EFF00560	EC042_RS24195	-	-	T6SS00463	Escherichia coli 042	chromosome (NC_017626)	4858680..4859171 (+)	387609950
EFF00561	EC042_4564 (AaiC)	-	-	T6SS00464	Escherichia coli 042	chromosome (NC_017626)	4895043..4895549 (+)	387609980
EFF01309	xmoF (Hcp1)	-	-	T6SS00904	Escherichia coli strain TW-XM	chromosome (KF678349)	5838..6329 (+)	611963684
EFF01479	xmtA (Hcp2A)	-	-	T6SS00905	Escherichia coli strain TW-XM	chromosome (KF678350)	1..480 (-)	611963703
EFF01480	xmtR (Hcp2B)	-	-	T6SS00905	Escherichia coli strain TW-XM	chromosome (KF678350)	20330..20848 (+)	611963720
EFF01440	A364_RS23060	-	-	T6SS00930	Escherichia coli SEPT362	chromosome (NZ_AOGL01000004)	31762..32280 (+)	447065702
EFF01839	UG97_RS03505	-	-	T6SS01282	Kosakonia oryzae strain KO348	chromosome (NZ_JZLI01000002)	281642..283567 (+)	521291363
EFF01840	UG97_RS09005	-	-	T6SS01282	Kosakonia oryzae strain KO348	chromosome (NZ_JZLI01000006)	149587..152196 (-)	780193605
EFF01841	UG97_RS00630	-	-	T6SS01282	Kosakonia oryzae strain KO348	chromosome (NZ_JZLI01000001)	121734..123386 (-)	780188092
EFF01842	UG97_RS09090	-	-	T6SS01282	Kosakonia oryzae strain KO348	chromosome (NZ_JZLI01000006)	162910..165141 (-)	780193664
EFF18621	KPHS_23105 (Tle1^KP)	Tle	IMU17297	T6SS00548	Klebsiella pneumoniae subsp. pneumoniae HS11286	chromosome (NC_016845)	2341000..2343418 (+)	-
EFF76633	hcp-2	-	-	T6SS17502	Citrobacter freundii strain CF74	chromosome (-)	-	-
EFF28264	PPECC33_RS03580 (Rhs3)	-	IMU38222	T6SS01034	Escherichia coli PCN033	chromosome (NZ_CP006632)	774741..778916 (+)	WP_047938335.1
EFF28267	PPECC33_RS07930 (Rhs2)	-	IMU38223	T6SS01034	Escherichia coli PCN033	chromosome (NZ_CP006632)	1615760..1619914 (+)	WP_047938343.1
EFF28262	PPECC33_RS01260 (Rhs1)	-	-	T6SS01034	Escherichia coli PCN033	chromosome (NZ_CP006632)	270305..274567 (+)	WP_047938332.1
EFF28269	PPECC33_RS10745 (Rhs4)	-	-	T6SS01034	Escherichia coli PCN033	chromosome (NZ_CP006632)	2169467..2173648 (-)	WP_047938391.1

Experimental investigation has been performed.

Bioinformatics investigation has been performed

Immunity protein ID	Locus tag (Gene)	Related T6SS	Organism	Replicon (Accession)	Coordinates (Strand)	Protein GI	Cognate effector
IMU00055	ECL_01568 (RhsIA)	T6SS00395	Enterobacter cloacae subsp. cloacae ATCC 13047	chromosome (NC_014121)	1595931..1596929 (+)	296101930	EFF01254
IMU00056	ECL_03139 (RhsIB)	T6SS00395	Enterobacter cloacae subsp. cloacae ATCC 13047	chromosome (NC_014121)	3199746..3200309 (-)	296103478	EFF01255
IMU00254	XmoJ (Tli4)	T6SS00904	Escherichia coli strain TW-XM	chromosome (KF678349)	13422..14132 (+)	611963688	EFF01348
IMU00269	ECL_01543 (Tai4)	T6SS00395	Enterobacter cloacae subsp. cloacae ATCC 13047	chromosome (NC_014121)	1563960..1564313 (+)	296101905	EFF01418
IMU00303	STM0278 (Tai4)	T6SS00535	Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344	chromosome (NC_016810)	318129..318512 (+)	378698250	EFF01370
IMU00313	EC042_4535 (Tli1EAEC)	T6SS00463	Escherichia coli 042	chromosome (NC_017626)	4867307..4867984 (+)	446118504	EFF01465
IMU00321	AOM81336 (CTI1)	T6SS00941	Escherichia coli strain STEC004	chromosome (KX118288)	7270..7521 (+)	1062685108	EFF01518
IMU00322	het3	T6SS00944	Escherichia coli strain PE086	chromosome (KX118289)	1861..2145 (+)	1062685125	EFF01522
IMU00323	het5	T6SS00944	Escherichia coli strain PE086	chromosome (KX118289)	2553..2843 (+)	1062685127	EFF01522
IMU00324	het6	T6SS00944	Escherichia coli strain PE086	chromosome (KX118289)	3252..3542 (+)	1062685128	EFF01522
IMU00327	XmtV	T6SS00905	Escherichia coli strain TW-XM	chromosome (KF678350)	25611..25901 (+)	611963724	EFF01498
IMU00328	vt6C (VTI1)	T6SS00947	Escherichia coli strain PE050	chromosome (KX118292)	3177..3626 (+)	1062685203	EFF01527
IMU00329	vt4C (VTI5)	T6SS00946	Escherichia coli strain PE117	chromosome (KX118293)	3140..3691 (+)	1062685230	EFF01525
IMU00342	ECO111_0593 (CTI3)	T6SS00367	Escherichia coli O111:H- str. 11128	chromosome (NC_013364)	632211..632576 (-)	446014673	EFF01519
IMU00343	Z5015 (CTI5)	T6SS00006	Escherichia coli O157:H7 str. EDL933	chromosome (NC_002655)	4576267..4577109 (+)	445994995	EFF01452
IMU00344	AAF13_24845 (Hcp-ETI1)	-	Escherichia coli O104:H4 str. C227-11	chromosome (CP011331)	4946440..4947108 (+)	816213339	EFF01520
IMU00345	A31K_02347 (Hcp-ET2)	T6SS01200	Escherichia coli KTE165	chromosome (NZ_KB733141)	2548806..2550806 (+)	486402833	EFF01521
IMU00349	BN49_RS18785 (Sel1E)	T6SS01216	Klebsiella pneumoniae strain Kp52.145	chromosome (NZ_FO834906)	3496153..3497427 (-)	802146974	EFF01826
IMU00372	STM14_0335 (Tldi1)	T6SS00551	Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S	chromosome (NC_016856)	329200..329616 (+)	267991976	-
IMU00375	ECL_01554 (Tli)	T6SS00395	Enterobacter cloacae subsp. cloacae ATCC 13047	chromosome (NC_014121)	1577823..1578584 (+)	296101916	EFF01872
IMU17297	KPHS_23060 (Tli1^KP)	T6SS00548	Klebsiella pneumoniae subsp. pneumoniae HS11286	chromosome (NC_016845)	2338687..2339217 (+)	YP_005226606.1	EFF18621
IMU17298	KPHS_23070 (Tli2^KP)	T6SS00548	Klebsiella pneumoniae subsp. pneumoniae HS11286	chromosome (NC_016845)	2339286..2339816 (+)	YP_005226607.1	EFF18621
IMU17299	KPHS_23080 (Tli3^KP)	T6SS00548	Klebsiella pneumoniae subsp. pneumoniae HS11286	chromosome (NC_016845)	2339884..2340414 (+)	YP_005226608.1	EFF18621
IMU17300	KPHS_23090 (Tli4^KP)	T6SS00548	Klebsiella pneumoniae subsp. pneumoniae HS11286	chromosome (NC_016845)	2340482..2341012 (+)	YP_005226609.1	EFF18621
IMU38222	PPECC33_RS03585 (Rhs3I)	T6SS01034	Escherichia coli PCN033	chromosome (NZ_CP006632)	778901..779275 (+)	WP_000494464.1	EFF28264
IMU38223	PPECC33_RS07935 (Rhs2I)	T6SS01034	Escherichia coli PCN033	chromosome (NZ_CP006632)	1619941..1620285 (+)	WP_001279013.1	EFF28267

Experimental investigation has been performed.

Bioinformatics investigation has been performed

ID	Locus tag (Gene)	Organism	Replicon (Accession)	Coordinates (Strand)	Protein GI	Response to T6SS attack	Known or putative function
RSF000001	BW25113_RS11580 (RcsB/ymgA)	Escherichia coli BW25113 strain K-12	chromosome (NZ_CP009273)	2309656..2310306 (+)	446984661	protective	RcsB regulon; response envelope stress
RSF000002	BW25113_RS11585 (RcsC)	Escherichia coli BW25113 strain K-12	chromosome (NZ_CP009273)	2310506..2313355 (-)	446798755	protective	RcsB regulon; response envelope stress
RSF000003	BW25113_RS00985 (RcsF)	Escherichia coli BW25113 strain K-12	chromosome (NZ_CP009273)	216078..216482 (-)	447125073	protective	RcsB regulon; response envelope stress
RSF000004	BW25113_RS10750 (wcaA)	Escherichia coli BW25113 strain K-12	chromosome (NZ_CP009273)	2126039..2126878 (-)	446717386	protective	RcsB regulon response envelope stress
RSF000005	BW25113_RS00275 (surA)	Escherichia coli BW25113 strain K-12	chromosome (NZ_CP009273)	53416..54702 (-)	446723144	protective	Menbtane integrity
RSF000006	BW25113_RS03235 (pagP)	Escherichia coli BW25113 strain K-12	chromosome (NZ_CP009273)	652013..652573 (+)	447025838	protective	Menbtane integrity
RSF000007	BW25113_RS00460 (ddlB)	Escherichia coli BW25113 strain K-12	chromosome (NZ_CP009273)	98720..99640 (+)	446052201	protective	peptidoglycan synthesis
RSF000008	BW25113_RS10855 (BaeR)	Escherichia coli BW25113 strain K-12	chromosome (NZ_CP009273)	2157757..2158479 (+)	446060022	protective	envelope stress response
RSF000009	BW25113_RS18230 (gadA)	Escherichia coli BW25113 strain K-12	chromosome (NZ_CP009273)	3659540..3660940 (-)	446294385	protective	Acid stress response
RSF000010	BW25113_RS07825 (gadB)	Escherichia coli BW25113 strain K-12	chromosome (NZ_CP009273)	1564902..1566302 (-)	446281075	protective	Acid stress response
RSF000011	BW25113_RS07820 (gadC)	Escherichia coli BW25113 strain K-12	chromosome (NZ_CP009273)	1563211..1564746 (-)	446168164	protective	Acid stress response
RSF000012	BW25113_RS18200 (gadE)	Escherichia coli BW25113 strain K-12	chromosome (NZ_CP009273)	3651726..3652253 (+)	446498836	protective	Acid stress response
RSF000013	BW25113_RS10355 (YeeN)	Escherichia coli BW25113 strain K-12	chromosome (NZ_CP009273)	2050339..2051055 (+)	446455068	sensitive	YebC/PmpR family DNA-binding transcriptional regulator
RSF000014	BW25113_RS02065 (phoB)	Escherichia coli BW25113 strain K-12	chromosome (NZ_CP009273)	412598..413287 (+)	446036078	sensitive	phosphate response regulator transcription factor
RSF000015	BW25113_RS06710 (osmB)	Escherichia coli BW25113 strain K-12	chromosome (NZ_CP009273)	1337367..1337585 (-)	446420398	protective	Osmotic stress response
RSF000016	BW25113_RS07770 (osmC)	Escherichia coli BW25113 strain K-12	chromosome (NZ_CP009273)	1550882..1551313 (+)	446074450	protective	Osmotic stress response
RSF000017	BW25113_RS22785 (osmY)	Escherichia coli BW25113 strain K-12	chromosome (NZ_CP009273)	4601213..4601818 (+)	481041372	protective	Osmotic stress response
RSF000018	BW25113_RS03840 (tolB)	Escherichia coli BW25113 strain K-12	chromosome (NZ_CP009273)	773196..774488 (+)	481023805	protective	Tol-Pal system protein
RSF000019	BW25113_RS13420 (rseA)	Escherichia coli BW25113 strain K-12	chromosome (NZ_CP009273)	2702113..2702763 (-)	447091203	protective	anti-sigma-E factor
RSF000027	BW25113_RS00810 (degP)	Escherichia coli BW25113 strain K-12	chromosome (NZ_CP009273)	177371..178795 (+)	446676600	protective	Maintaining envelope integrity
RSF000028	BW25113_RS10765 (wza)	Escherichia coli BW25113 strain K-12	chromosome (NZ_CP009273)	2129585..2130724 (-)	446900838	protective	colanic acid synthesis
RSF000029	BW25113_RS07765 (bdm)	Escherichia coli BW25113 strain K-12	chromosome (NZ_CP009273)	1550322..1550537 (-)	446417916	protective	-
RSF000030	BW25113_RS10200 (RcsA)	Escherichia coli BW25113 strain K-12	chromosome (NZ_CP009273)	2017449..2018072 (+)	446026146	protective	RcsB regulon response envelope stress
RSF000031	BW25113_RS10690 (wcaJ)	Escherichia coli BW25113 strain K-12	chromosome (NZ_CP009273)	2113641..2115035 (-)	446105252	protective	RcsB regulon response envelope stress
RSF000032	BW25113_RS09120 (Spy)	Escherichia coli BW25113 strain K-12	chromosome (NZ_CP009273)	1819397..1819882 (-)	447151728	protective	Maintaining envelope integrity
RSF000035	BW25113_RS02265 (clpP)	Escherichia coli BW25113 strain K-12	chromosome (NZ_CP009273)	452133..452756 (+)	446044398	sensitive	serine protease
RSF000036	BW25113_RS04580 (ClpA)	Escherichia coli BW25113 strain K-12	chromosome (NZ_CP009273)	918720..920996 (+)	446856785	sensitive	serine protease adapter
RSF000037	BW25113_RS03725 (gltA)	Escherichia coli BW25113 strain K-12	chromosome (NZ_CP009273)	748641..749924 (-)	446708488	sensitive	citrate synthase
RSF000038	BW25113_RS08730 (ydhS)	Escherichia coli BW25113 strain K-12	chromosome (NZ_CP009273)	1741388..1742992 (+)	446639604	sensitive	FAD/NAD(P) binding domain-containing protein
RSF000039	BW25113_RS07100 (ydaE)	Escherichia coli BW25113 strain K-12	chromosome (NZ_CP009273)	1412095..1412265 (-)	485720780	sensitive	Rac prophage; zinc-binding protein
RSF000040	BW25113_RS05200 (cbpA)	Escherichia coli BW25113 strain K-12	chromosome (NZ_CP009273)	1058311..1059231 (-)	446342766	sensitive	Curved DNA-binding protein
RSF000041	DK885_RS15130 (OmpA)	Escherichia coli strain C600	chromosome (NZ_CP031214)	2901443..2902483 (+)	446673070	protective	outer membrane stability
RSF000042	BN896_RS02380 (ompC)	Escherichia coli str. K-12 substr. MC4100 strain K-12	chromosome (NZ_HG738867)	477741..478808 (-)	446659936	protective	outer membrane stability
RSF000043	BN896_RS04350 (ompF)	Escherichia coli str. K-12 substr. MC4100 strain K-12	chromosome (NZ_HG738867)	888085..889173 (-)	446900664	protective	outer membrane stability
RSF000044	BN896_RS02450 (ompT)	Escherichia coli str. K-12 substr. MC4100 strain K-12	chromosome (NZ_HG738867)	486663..487616 (-)	447124589	protective	outer membrane stability
RSF000045	DK885_RS08170 (eco)	Escherichia coli strain C600	chromosome (NZ_CP031214)	1618127..1618615 (-)	446771953	protective	serine protease inhibitor
RSF000046	DK885_RS22260 (lamB)	Escherichia coli strain C600	chromosome (NZ_CP031214)	4276516..4277856 (-)	446896407	sensitive	maltose regulon
RSF000047	BN896_RS20425 (lamB)	Escherichia coli str. K-12 substr. MC4100 strain K-12	chromosome (NZ_HG738867)	4133927..4135267 (+)	446896407	sensitive	maltose regulon
RSF000048	b4036 (lamB)	Escherichia coli str. K-12 substr. MG1655	chromosome (NC_000913)	4247971..4249311 (+)	16131862	sensitive	maltose regulon
RSF000049	b3357 (crp)	Escherichia coli str. K-12 substr. MG1655	chromosome (NC_000913)	3486120..3486752 (+)	16131236	sensitive	cAMP regulon
RSF000056	KPST380_RS07600 (wza)	Klebsiella pneumoniae subsp. pneumoniae BJ1-GA	chromosome (NZ_HG518479)	3683821..3684957 (-)	WP_004180522.1	protective	capsule protection
RSF000057	KP1_RS17340 (wza)	Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044	chromosome (NC_012731)	3540983..3542116 (-)	WP_014907221.1	protective	capsule protection
RSF000058	KPK_RS07975 (wza)	Klebsiella variicola	chromosome (NC_011283)	1699259..1700392 (+)	WP_012541078.1	protective	capsule protection
RSF000059	LGM18_RS08295 (wza)	Klebsiella pneumoniae strain SB617	chromosome (NZ_CP084825)	1719795..1720931 (+)	WP_136085646.1	protective	capsule protection
RSF000060	KPNJ1_RS09040 (wza)	Klebsiella pneumoniae 30660/NJST258_1	chromosome (NZ_CP006923)	1798389..1799522 (+)	WP_004151152.1	protective	capsule protection
RSF000061	BSJ02_RS22110 (wza)	Escherichia coli strain MOD1-EC300	chromosome (NZ_NJMM01000062)	16803..17939 (-)	WP_096838211.1	protective	capsule protection
RSF000062	BSJ02_RS12510 (rfaH)	Escherichia coli strain MOD1-EC300	chromosome (NZ_NJMM01000018)	10609..11097 (+)	WP_096838056.1	protective	capsule protection

Experimental investigation has been performed.

Bioinformatics investigation has been performed

Accessory protein ID	Locus tag (Gene)	Related T6SS	Type	Organism	Replicon (Accession)	Coordinates (Strand)	Protein GI	Related structure protein	Related effector
ACP00024	ECO111_0594 (eagR)	T6SS00367	adaptor	Escherichia coli O111:H- str. 11128	chromosome (NC_013364)	632578..637377 (-)	447211780	ECO111_0596 (VgrGO1)	ECO111_0594 (Rhs-CT3)
ACP00032	EC042_RS24300 (tagA)	T6SS00931	structure affiliated protein	Escherichia coli 042	chromosome (NC_017626)	4884070..4885437 (+)	446122356	TssA	-
ACP00033	EC042_RS24190 (tagL/ompA)	T6SS00931	structure affiliated protein	Escherichia coli 042	chromosome (NC_017626)	4856944..4858674 (+)	446480629	TssL	peptidoglycan
ACP00034	EC042_1244 (mltE)	T6SS00931	structure affiliated protein	Escherichia coli 042	chromosome (NC_017626)	1322321..1322932 (+)	481024453	TssM	-
ACP00035	EC042_RS24190 (sciZ)	T6SS00931	structure affiliated protein	Escherichia coli 042	chromosome (NC_017626)	4856944..4858674 (+)	446480629	SciP	peptidoglycan
ACP00036	EC042_RS21585 (yidC)	T6SS00931	chaperone	Escherichia coli 042	chromosome (NC_017626)	4320019..4321665 (+)	446300403	TssL	-
ACP00037	EC042_RS00085 (dnaK)	T6SS00931	chaperone	Escherichia coli 042	chromosome (NC_017626)	13600..15516 (+)	446438280	TssL	-
ACP00038	AOM81334 (eagR/DUF1795)	T6SS00941	adaptor	Escherichia coli strain STEC004	chromosome (KX118288)	1930..2391 (+)	1062685106	ct1A (VgrGO1)	ct1C (Rhs-CT1)