Detailed information of Recipient susceptibility factor


Summary

ID RSF000057 experimental
Strain Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044
Replicon chromosome
Sequence Protein sequence (378 a.a.); Nucleotide sequence (1134 bp)
Response to T6SS attack protective
Known or putative function capsule protection
Description Group I capsules confer a strong protection from V. cholerae's T6SS attacks to commensal Klebsiella strains.
Reference
experimental Experimental investigation has been performed.
External database links

Locus tag (Gene) KP1_RS17340 (wza)
Coordinates (Strand) 3540983..3542116 (-)
NCBI ID WP_014907221.1
RefSeq NC_012731
Uniprot ID A0A652PV82_9ENTR, A0A0P0YQD6_KLEPN, Q5XL55_KLEPN, A0A3F3B359_KLEPN
KEGG ID kpu:KP1_3720
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Poly_export PF02563.18 1.2e-27 82..165
Wza_C PF18412.3 2.3e-17 345..374
SLBB PF10531.11 6.1e-08 173..222
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-4 MKKK
Transmembrane helix 5-27 IVKISALALVIGFLSGCTIIPGQ
Outside 28-377 GLNSLRKNVVELPDSDYDLDKLVNVYPMTPGLIDQLRPESVLARPNPQLDNLLRSYEYRIGVGDVLMVTVWDHPELTTPAGQYRSASDTGNWVNSDGTIFYPYVGKVQVAGKTLSQVRQDIASRLTTYIESPQVDVSIASFRSQKTYVTGEVVKSGQQPITNIPLTVMDAINAAGGLAPDADWRNVVLTHNGKDTKISLYALMQKGDLTQNHLLYPGDILFVPRNDDLKVFVMGEVGKQSTMKMDRSGMTLAEAIGNAEGMSQALSDATGVFVIRQLKGDKQGKIANVYQLNAKDASAMVLSTEFALQPYDIVYVTAAPVVRWNRVVSQLVPTITGVHDMTEAARYVRNW


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
LIPO(Sec/SPII) 0.9984 pos: 20-21. LSG-CT. MKKKIVKISALALVIGFLSG


  Protein sequence: 378 a.a.    .

>RSF000057 NC_012731:c3542116-3540983 [Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044]
MKKKIVKISALALVIGFLSGCTIIPGQGLNSLRKNVVELPDSDYDLDKLVNVYPMTPGLIDQLRPESVLARPNPQLDNLL
RSYEYRIGVGDVLMVTVWDHPELTTPAGQYRSASDTGNWVNSDGTIFYPYVGKVQVAGKTLSQVRQDIASRLTTYIESPQ
VDVSIASFRSQKTYVTGEVVKSGQQPITNIPLTVMDAINAAGGLAPDADWRNVVLTHNGKDTKISLYALMQKGDLTQNHL
LYPGDILFVPRNDDLKVFVMGEVGKQSTMKMDRSGMTLAEAIGNAEGMSQALSDATGVFVIRQLKGDKQGKIANVYQLNA
KDASAMVLSTEFALQPYDIVYVTAAPVVRWNRVVSQLVPTITGVHDMTEAARYVRNW*

  Nucleotide sequence: 1134 bp    .

>RSF000057 NC_012731:c3542116-3540983 [Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044]
ATGAAGAAAAAAATTGTTAAAATTTCGGCATTAGCGTTGGTAATCGGCTTTTTATCGGGCTGTACCATTATCCCGGGTCA
GGGATTGAATAGTTTACGTAAGAACGTGGTTGAGCTACCGGATAGCGATTACGATCTGGATAAATTGGTCAATGTCTATC
CTATGACACCTGGGTTAATTGATCAGCTTCGTCCTGAATCTGTGTTAGCACGACCAAACCCGCAATTGGATAATTTATTG
CGTAGCTATGAATATCGTATTGGGGTTGGCGATGTACTTATGGTAACCGTATGGGATCACCCGGAATTGACTACTCCGGC
AGGCCAGTACCGTAGCGCGAGCGATACTGGTAACTGGGTTAATTCTGACGGAACTATTTTCTACCCCTATGTCGGTAAGG
TGCAAGTAGCTGGAAAAACCCTCAGTCAGGTACGCCAGGATATCGCCAGCAGATTAACGACTTATATTGAAAGCCCTCAG
GTTGATGTAAGCATAGCATCTTTTAGATCGCAAAAAACCTATGTTACAGGTGAAGTTGTTAAATCTGGTCAACAACCAAT
CACTAATATCCCGTTGACTGTAATGGATGCTATTAATGCCGCGGGTGGCTTGGCTCCTGACGCTGATTGGAGAAATGTCG
TTCTCACGCATAACGGTAAAGATACTAAGATCTCCTTATATGCCTTAATGCAAAAAGGTGATCTCACACAAAATCACTTG
CTTTATCCGGGTGATATTTTATTTGTACCACGAAATGATGATCTGAAAGTGTTTGTCATGGGCGAGGTAGGTAAACAAAG
CACCATGAAAATGGATCGCAGTGGGATGACGTTAGCTGAAGCTATAGGTAATGCTGAAGGAATGTCACAAGCATTGAGTG
ATGCGACAGGTGTATTTGTAATAAGACAGCTCAAAGGTGATAAGCAAGGTAAAATCGCTAATGTTTACCAATTGAATGCG
AAAGATGCATCTGCCATGGTACTAAGCACCGAATTTGCTTTACAACCTTACGACATTGTTTACGTTACAGCTGCTCCAGT
AGTACGTTGGAACCGTGTAGTCTCTCAATTGGTTCCAACTATTACCGGGGTACATGATATGACAGAAGCCGCGAGATATG
TAAGGAACTGGTAA