Type VI secretion system (T6SS) is a class of sophisticated protein delivering apparatus, usually composed of ~13 core components with or withnot accessory proteins in different species, involved in mediating antagonistic or synergistic communications between bacteria and/or bacteria and eukaryotes. This apparatus has been found to be widely distributed among Gram-negative bacterial species and divided into four classes: i, ii, iii, and iv. Type i T6SS is the most classic and widely studied, and is further subclassed: i1, i2, i3, i4a, i4b, i5. To date, only a few datasets of T6SS core components are available, such as KEGG and MicrobeWiki. Two comprehensive databases EffectiveDB and BastionHub are mainly focus on effectors of several secretion systems in bacteria, lacking specific and integrated full-scale data of T6SS. Here, we have organized information derived from the reported literature of T6SS gene cluster, T6SS regulator and accessory protein, as well as cognate effector, immunity and structure component protein, together with tools for T6SS classification and T6SS prediction as the web-based resource SecReT6 (Type VI Secretion system Resource).

T6SS cluster gene data were exhibited by five entrances: Taxonomy, Replicon, Classification, Function, and T6SS list. The whole T6SS data by experimental validated were exhibited in every organism via a summary thumbnail, users can view the information by clicking organism column, like Acinetobacter sp. ADP1 chromosome in T6SS list. The detailed information of T6SS and relationship between T6SS and T6SS proteins including effector (T6SE), immunity (T6SI), regulator (T6SR), accessory protein (T6SA), were showed by intuitive graph and diagram which users can get from T6SS ID column in T6SS list, like T6SS00061.

T6SS proteins were all exhibited detailed information and relationship with T6SS by intuitive graph and diagram through matching SecReT6 Protein ID or locus tag (Gene) in the sympathetic module, like Effector in Effector ID EFF01470, or locus tag (Gene) ACIAD_RS00785, could be browsed in Effector list. Effectors were classified by function type from Tle to Tse, which users can enter into through Function type in Effector of Browse module. T6SS Immunity module was added non-specific immunity protein or mechanism pathway, also named Recipient susceptibility factor, shown in Immunity list. T6SS regulators were classified by Regulatory pathway in Regulator of Browse module and T6SS accessory protein was constituted by adaptor, chaperone, and structure affiliated protein, shown in Accessory protein list of Browse module.

SecReT6 v3.0 serves a Search function in every webpage and data could be browsed through two entrances: Replicon or Taxonomy, to help users get information easily.

SecReT6: a web-based resource for type VI secretion systems found in bacteria

The open-access database SecReT6 offers a unique, readily explorable archive of known and putative T6SSs and cognate T6SS proteins including effectors, immunity proteins, regulators, and accessory proteins in bacteria. A broad range of T6SS gene cluster and T6SS protein prediction, classifiction, comparative analyses, similarity searches, phylogenetic analysis, and other functional analysis tools are readily accessible via SecReT6.

SecReT6 v1.0 released on Aug. 15th, 2013.
SecReT6 v1.0 contains data on 9,664 core T6SS components mapping to 782 T6SSs found in 431 bacterial strains representing 216 species, as well as a collection of over four hundred directly relevant references.

SecReT6 v2.0 released on Jun. 28th, 2014.
SecReT6 v2.0 currently contains data on 10,891 core T6SS components belonging to 873 T6SSs found in 478 bacterial strains representing 221 species, as well as a collection of over six hundred associated references. Also collated and archived were 1,277 diverse candidate secreted effectors which were experimentally demonstrated and/or predicted to be delivered by T6SSs into target eukaryotic and/or prokaryote cells as well as 174 immunity proteins.

SecReT6 v3.0 released on Jun. 28th, 2021.
SecReT6 v3.0 currently contains 207 experimental validated T6SSs and 17223 bioinformatic preidcited T6SSs in 27000 bacteria with features of chromsome or complete from NCBI, and also updated 287 experimentally validated and 18023 bioinformatically preidcited effectors, 121 experimentally validated and 17160 bioinformatically preidcited cognate immunity proteins. We added 51 recipient susceptibility factor protein in immunity module. SecReT6 v3.0 serves new data of T6SS regulators and T6SS accessory proteins which contains 340 and 44 proteins or RNA respectively. SecReT6 v3.0 has been refactored web interface and data enter, and added new tool to classify T6SS and all kinds of T6SS protein prediction.

Overview of the classic T6SS assembly mode

Overview of the T6SS regulatory mechanism in Pseudomonas aeruginosa

Overview of the T6SE vector assembly mode

T6SS conserved components

Table 1. List of core components found in the well-documented T6SS representatives
Component Function Cluster of Orthologous Groups
TssA Unknown COG3515
TssB (IglA, VipA) Tubular complex COG3516
TssC (IglB, VipB) Tubular complex COG3517
TssD (Hcp) Hexameric ring COG3157
TssE Cytoplasmic protein COG3518
TssF Unknown COG3519
TssG Unknown COG3520
TssH (ClpV) ATPase COG0542
TssI (VgrG) Needle-like structure COG3501
TssJ Transmembrane spanning structure COG3521
TssK Cytoplasmic protein COG3522
TssL (DotU) Transmembrane spanning structure COG3455
TssM (IcmF) Transmembrane spanning structure COG3523

More details at the Excel file.

The prediction strategy used by SecReT6 to identify T6SS-encoding loci

References of T6SS assembly mode

[1] Bernal, P., et al. (2021). A novel stabilization mechanism for the type VI secretion system sheath. PNAS, 118(7), 1-9.
[2] Wu, C.,et al. (2020). Effector loading onto the VgrG carrier activates type VI secretion system assembly. EMBO Reports, 21(1), 1-12.
[3] Wang, J., Brodmann, M., & Basler, M. (2019). Assembly and Subcellular Localization of Bacterial Type VI Secretion Systems. Annual Review of Microbiology, 73(1), 1-18.
[4] Pissaridou, P., et al. (2018). The Pseudomonas aeruginosa T6SS-VgrG1b spike is topped by a PAAR protein eliciting DNA damage to bacterial competitors. PNAS, 115 (49), 12519-12524.
[5] Burkinshaw, B. J., et al. (2018). A type VI secretion system effector delivery mechanism dependent on PAAR and a chaperone-co-chaperone complex. Nat Microbiol 3, 632-640.
[6] Yassine Cherrak Y, et al. (2018). Biogenesis and structure of a type VI secretion baseplate. Nat Microbiol 3, 1404-1416.
[7] Quentin, D., et al. (2018). Mechanism of loading and translocation of type VI secretion system effector Tse6. Nat Microbiol 3, 1142-1152.
[8] Unterweger, D., Kostiuk, B., & Pukatzki, S. (2017). Adaptor Proteins of Type VI Secretion System Effectors. Trends in Microbiology, 25(1), 8-10.
[9] Nguyen, V. S., et al. (2017). Type VI secretion TssK baseplate protein exhibits structural similarity with phage receptor-binding proteins and evolved to bind the membrane complex. Nat Microbiol, 2, 1-9.
[10] Whitney, J. C., et al. (2015). An Interbacterial NAD(P)+ Glycohydrolase Toxin Requires Elongation Factor Tu for Delivery to Target Cells. Cell, 163(3), 607-619.
[11] Durand, E., et al. (2015). Biogenesis and structure of a type VI secretion membrane core complex. Nature, 523(7562), 555-560.
[12] Mikhail M., et al. (2014). PAAR-repeat proteins sharpen and diversify the Type VI secretion system spike Mikhail. Nature, 500(7462), 350-353.