SecReT6, T6SS database

Detailed information of immunity protein

Summary

Immunity protein ID	IMU00254
Cognate effector	EFF01348
Related T6SS (Type)	T6SS00904 (Type i2)
Strain	Escherichia coli strain TW-XM
Replicon	chromosome
Sequence	Protein sequence (237 a.a.); Nucleotide sequence (711 bp)
Description	The antibacterial activity of the Tle4/Tli4 pair was markedly attenuated in the hcp1 mutant, and deletion of the Vs2 region (within loop L2, 3) in Hcp1 of APEC completely abolished the antibacterial capability of Tle4 against the mutant Δtle4Δtli4
Reference	[1] Ma J, Sun M, Pan Z, et al. Three Hcp homologs with divergent extended loop regions exhibit different functions in avian pathogenic Escherichia coli. Emerg Microbes Infect. 2018 Mar 29;7(1):49. doi: 10.1038/s41426-018-0042-0. PMID: 29593238

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	XmoJ (Tli4)
Coordinates (Strand)	13422..14132 (+)
NCBI ID	611963688
GenBank	KF678349
Uniprot ID	X5FD89_ECOLX
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
Tli4_N	PF18443.3	8.3e-19	56..182

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-11	MVKCHTLINRR
Transmembrane helix	12-29	NKCLLIVFIVLIGWIIFR
Outside	30-236	PKAYTYSLNDKEKEMLIMLSQHPETRYFGFYSIELPADYKPTGMVMFIQGSAMIPVETKLQYYPPFLQYMTRYEAELKNTSALDPLDTPYLKQVHPLSPPMNGVIFERMKAKYTPDFARVLDAWKWENGVTFSVKIEAKDGRATRYDGISKIAEYSYGYNIPEKKYSYLLFFQDYNLVQITNPHQKINWRYNMHRLTLHYLESMNYL

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.8822	-	-

Protein sequence: 237 a.a. .

                                    >IMU00254 KF678349:13422-14132 [Escherichia coli]
MVKCHTLINRRNKCLLIVFIVLIGWIIFRPKAYTYSLNDKEKEMLIMLSQHPETRYFGFYSIELPADYKPTGMVMFIQGS
AMIPVETKLQYYPPFLQYMTRYEAELKNTSALDPLDTPYLKQVHPLSPPMNGVIFERMKAKYTPDFARVLDAWKWENGVT
FSVKIEAKDGRATRYDGISKIAEYSYGYNIPEKKYSYLLFFQDYNLVQITNPHQKINWRYNMHRLTLHYLESMNYL*

                                

Nucleotide sequence: 711 bp .

                                    >IMU00254 KF678349:13422-14132 [Escherichia coli]
ATGGTAAAATGTCATACTCTGATTAACCGTAGAAATAAATGTCTGCTGATTGTTTTTATAGTCCTTATTGGATGGATTAT
ATTCAGACCTAAAGCATATACTTATTCACTAAATGATAAAGAAAAAGAGATGCTCATAATGTTATCACAACATCCTGAAA
CTCGGTACTTTGGATTTTATTCCATAGAACTTCCGGCTGATTACAAACCAACAGGAATGGTTATGTTCATACAAGGATCG
GCGATGATCCCTGTAGAAACAAAGCTACAATATTATCCTCCTTTTCTGCAATATATGACACGATATGAGGCAGAACTAAA
AAACACCTCAGCATTAGATCCACTGGATACGCCTTATTTGAAGCAAGTTCACCCACTAAGTCCACCTATGAATGGAGTCA
TTTTTGAACGAATGAAAGCGAAATACACCCCAGATTTTGCACGAGTATTGGATGCATGGAAATGGGAAAATGGCGTTACG
TTTTCAGTAAAAATAGAAGCTAAAGATGGTAGAGCAACCCGCTATGATGGAATTAGTAAGATTGCCGAATACAGTTATGG
ATATAATATTCCAGAAAAAAAGTACAGTTACTTACTATTCTTTCAGGACTACAACCTCGTGCAGATAACCAACCCCCATC
AGAAAATAAATTGGCGATACAATATGCACAGGTTGACGCTTCACTACTTGGAGAGTATGAATTATCTGTAG

                                