SecReT6, T6SS database

Detailed information of Recipient susceptibility factor

Summary

ID	RSF000029
Strain	Escherichia coli BW25113 strain K-12
Replicon	chromosome
Sequence	Protein sequence (72 a.a.); Nucleotide sequence (216 bp)
Response to T6SS attack	protective
Known or putative function	-
Description	osmB and bdm, which respond to osmotic stress, a potential consequence of peptidoglycan damage regulated by RcsB played a role in protecting against TseH-mediated killing, as the survival of the mutant strains was significantly reduced.
Reference	[1] Hersch SJ, Watanabe N, Stietz MS, et al. Envelope stress responses defend against type six secretion system attacks independently of immunity proteins. Nat Microbiol. 2020 May;5(5):706-714. doi: 10.1038/s41564-020-0672-6. Epub 2020 Feb 24. PMID: 32094588 [2] Kamal F, Liang X, Manera K, et al. Differential Cellular Response to Translocated Toxic Effectors and Physical Penetration by the Type VI Secretion System. Cell Rep. 2020 Jun 16;31(11):107766. doi: 10.1016/j.celrep.2020.107766. PMID: 32553162 [3] Hersch SJ, Manera K, Dong TG. Defending against the Type Six Secretion System: beyond Immunity Genes. Cell Rep. 2020 Oct 13;33(2):108259. doi: 10.1016/j.celrep.2020.108259. PMID: 33053336

Experimental investigation has been performed.

External database links

Locus tag (Gene)	BW25113_RS07765 (bdm)
Coordinates (Strand)	1550322..1550537 (-)
NCBI ID	446417916
RefSeq	NZ_CP009273
Uniprot ID	M9GCH0_ECOLX, S1F9K8_ECOLX, S1HYW8_ECOLX, V0AC38_ECOLX, A0A0A0FG82_ECOLX, E3PLY1_ECOH1, A0A0E1STS5_ECOLX, A0A140NA01_ECOBD, A0A1X3JHG6_ECOLX, F4SIR9_ECOLX, E6BQQ1_ECOLX, E1IT51_ECOLX, E1JCP7_ECOLX, A0A0E1M1P5_ECOLX, D7XVX3_ECOLX, D7Y0D4_ECOM1, BDM_ECOLI
KEGG ID	eco:b1481, ebd:ECBD_2158, ecj:JW5239, elh:ETEC_1551
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
BDM	PF10684.11	2.7e-52	1..71

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-71	MFTYYQAENSTAEPALVNAIEQGLRAQHGVVTEDDILMELTKWVEASDNDILSDIYQQTINYVVSGQHPTL

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9902	-	-

Protein sequence: 72 a.a. .

                                    >RSF000029 NZ_CP009273:c1550537-1550322 [Escherichia coli BW25113]
MFTYYQAENSTAEPALVNAIEQGLRAQHGVVTEDDILMELTKWVEASDNDILSDIYQQTINYVVSGQHPTL*

Nucleotide sequence: 216 bp .

                                    >RSF000029 NZ_CP009273:c1550537-1550322 [Escherichia coli BW25113]
ATGTTTACTTATTATCAGGCAGAAAATTCAACAGCAGAACCCGCTCTGGTTAATGCCATTGAGCAAGGCCTTCGGGCACA
GCATGGTGTTGTCACTGAAGATGACATTCTCATGGAGCTGACCAAATGGGTTGAAGCTTCTGATAACGACATCCTCAGTG
ATATCTACCAGCAAACGATCAATTATGTGGTCAGTGGCCAGCACCCTACGCTTTAA

                                