Detailed information of effector


Summary

Effector ID EFF01521 experimental
Effector type Tle
Cognate immunity protein IMU00345 experimental
Related T6SS (Type) T6SS01200 experimental (Type i2)
Strain Escherichia coli KTE165
Replicon chromosome
Sequence Protein sequence (667 a.a.); Nucleotide sequence (2001 bp)
Description Hcp-ET2 contains a C-terminal DUF2235 domain,encodes a conserved GxSxG sequence, which has been identified as the catalytic motif of Tle1 proteins
Reference
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed
External database links

Locus tag (Gene) A31K_02346 (Hcp-ET2)
Coordinates (Strand) 2548806..2550806 (+)
NCBI ID 431425568
RefSeq NZ_KB733141
Uniprot ID UPI0002A213E7
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS_HCP PF05638.14 6.6e-13 6..137
DUF2235 PF09994.11 6.7e-12 368..460
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-666 MGDIIYLRITGEQQGDISAGCGTQVSVGNRYQQGHEDEIFVFSFQGGVSNTGFGINHQAIQFCKILDKSSPLLMNCINNNERCRFEFYFYRINKYGKWERYYYIEVRGATLTQNQIIIKENELDYQYITIHYEYIYCKHLTANTEFSYLLTPENYNRLFPPTLLPVEEKPEIPPEREIILTIGVFFDGTGNNLTNTNLRMSFCQPETYGLDVQDLASFNKQCMSKQGKTGSGVQSYLNYYTNIHWLNKLYHKDSEINAKSAKIQREIYIEGIGTENNKADSLVGMGLGNNDTGVIAKTDRAISLLRETLAKIIVDLKDAKLVIKRLQFDVFGFSRGAAAARHFTNRVFERDPVLIKTIATAFQSVEYLGKPSGEVQFLGIFDTVAAIGGLMDGFDPHDGNNFPVKVKLPPGVAKHVFQLTAMNECRYNFCLNSIKESWPELSFPGSHADIGGGYDPLEDEYLFLSRPAIETVLGNVPVEATSAYQKAAQQAELLWRHSTLAPILPSGVLKIESNIDEIASPDHLGNVRKRVAAAVTFRRTITNDWSKIALRVMYEVAKEAGVVFDEIPDDDYYKYPEELSKICDKSISQAKDSLKKSTPQPFSSEELLVAGKYLHCSSNWNTVDYRLKNKVSAEVSAFKTFSFVNRPDENWTRTIYDMQGNKQNGI


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9683 - -


  Protein sequence: 667 a.a.    .

>EFF01521 NZ_KB733141:2548806-2550806 Type VI Lipase Effector (Tle) [Escherichia coli KTE165]
MGDIIYLRITGEQQGDISAGCGTQVSVGNRYQQGHEDEIFVFSFQGGVSNTGFGINHQAIQFCKILDKSSPLLMNCINNN
ERCRFEFYFYRINKYGKWERYYYIEVRGATLTQNQIIIKENELDYQYITIHYEYIYCKHLTANTEFSYLLTPENYNRLFP
PTLLPVEEKPEIPPEREIILTIGVFFDGTGNNLTNTNLRMSFCQPETYGLDVQDLASFNKQCMSKQGKTGSGVQSYLNYY
TNIHWLNKLYHKDSEINAKSAKIQREIYIEGIGTENNKADSLVGMGLGNNDTGVIAKTDRAISLLRETLAKIIVDLKDAK
LVIKRLQFDVFGFSRGAAAARHFTNRVFERDPVLIKTIATAFQSVEYLGKPSGEVQFLGIFDTVAAIGGLMDGFDPHDGN
NFPVKVKLPPGVAKHVFQLTAMNECRYNFCLNSIKESWPELSFPGSHADIGGGYDPLEDEYLFLSRPAIETVLGNVPVEA
TSAYQKAAQQAELLWRHSTLAPILPSGVLKIESNIDEIASPDHLGNVRKRVAAAVTFRRTITNDWSKIALRVMYEVAKEA
GVVFDEIPDDDYYKYPEELSKICDKSISQAKDSLKKSTPQPFSSEELLVAGKYLHCSSNWNTVDYRLKNKVSAEVSAFKT
FSFVNRPDENWTRTIYDMQGNKQNGI*

  Nucleotide sequence: 2001 bp    .

>EFF01521 NZ_KB733141:2548806-2550806 Type VI Lipase Effector (Tle) [Escherichia coli KTE165]
ATGGGTGACATTATATATTTACGCATTACAGGGGAGCAACAAGGGGATATTTCTGCAGGATGCGGGACGCAAGTCTCTGT
CGGGAATCGGTATCAACAGGGACATGAGGATGAAATATTTGTTTTTAGTTTTCAGGGAGGAGTAAGTAACACAGGATTTG
GCATTAATCATCAAGCGATACAATTTTGTAAAATTCTCGATAAAAGCTCACCGCTGCTGATGAATTGCATTAATAATAAC
GAACGATGCCGTTTTGAGTTTTATTTTTATCGTATTAATAAATATGGAAAATGGGAAAGGTATTATTATATTGAAGTCCG
TGGTGCCACGTTAACTCAGAATCAGATTATAATAAAAGAAAATGAACTCGACTATCAGTATATAACAATACACTACGAAT
ATATCTACTGTAAGCATCTTACAGCCAATACAGAATTTAGTTATTTACTCACACCTGAAAATTATAATCGCCTTTTCCCA
CCGACGCTCTTACCTGTCGAGGAAAAACCAGAAATACCGCCTGAACGAGAAATTATCCTCACCATTGGCGTATTCTTTGA
TGGCACAGGAAACAATTTAACCAATACGAATTTACGTATGAGTTTTTGCCAACCAGAAACCTATGGTCTGGATGTCCAGG
ATTTGGCCTCATTTAATAAACAATGTATGAGCAAGCAGGGTAAAACGGGATCCGGAGTACAGAGCTATTTGAATTACTAT
ACGAATATTCATTGGTTGAATAAGTTGTACCATAAAGACTCAGAAATAAATGCGAAGTCAGCTAAAATCCAACGTGAAAT
TTATATTGAAGGAATAGGCACTGAAAATAATAAAGCAGACTCTCTGGTAGGAATGGGGCTAGGAAATAATGATACTGGGG
TAATTGCCAAAACTGACCGTGCGATATCACTGCTTCGTGAGACACTGGCAAAGATTATTGTTGATTTAAAGGATGCTAAA
CTAGTAATTAAGCGTCTACAGTTTGACGTATTTGGCTTTAGTCGTGGGGCGGCAGCCGCACGGCACTTTACTAATCGCGT
ATTTGAACGTGATCCCGTACTGATTAAAACCATTGCGACGGCATTTCAGTCAGTTGAATATTTAGGTAAACCTTCCGGGG
AAGTGCAATTTTTAGGTATTTTTGACACAGTTGCAGCCATCGGAGGGCTGATGGATGGTTTTGACCCTCATGATGGTAAT
AACTTTCCGGTAAAAGTGAAGTTACCGCCAGGTGTGGCCAAACATGTATTCCAGCTCACAGCAATGAACGAATGTCGCTA
TAACTTTTGCCTCAACAGCATAAAGGAAAGCTGGCCAGAATTAAGTTTCCCTGGATCACATGCTGATATTGGTGGCGGCT
ATGACCCACTGGAAGATGAGTACCTGTTTTTAAGTCGCCCGGCGATTGAAACGGTACTGGGAAATGTGCCGGTGGAAGCG
ACAAGCGCTTACCAAAAAGCAGCACAGCAGGCAGAGTTATTGTGGCGCCATTCAACGCTGGCTCCGATTCTGCCTTCTGG
TGTCCTAAAAATTGAATCCAATATTGATGAAATAGCGTCGCCTGACCACTTAGGTAATGTGAGAAAACGGGTCGCTGCAG
CGGTGACATTTCGCAGAACGATCACCAATGACTGGTCAAAGATTGCGTTAAGAGTAATGTATGAAGTCGCTAAAGAGGCA
GGGGTAGTATTTGATGAAATACCAGATGATGATTATTATAAATATCCAGAGGAGTTAAGTAAAATATGTGATAAATCAAT
ATCACAAGCTAAAGATTCATTGAAAAAATCTACGCCACAACCTTTTTCTTCAGAAGAGTTACTCGTTGCCGGAAAATATT
TACACTGTTCTTCCAACTGGAATACCGTCGATTATCGATTAAAAAATAAGGTGTCTGCTGAAGTATCAGCGTTTAAGACG
TTTAGTTTTGTTAATCGGCCGGATGAAAATTGGACCAGAACTATATACGATATGCAAGGGAATAAACAAAATGGAATTTA
A