Detailed information of Recipient susceptibility factor

Summary

ID	RSF000003
Strain	Escherichia coli BW25113 strain K-12
Replicon	chromosome
Sequence	Protein sequence (135 a.a.); Nucleotide sequence (405 bp)
Response to T6SS attack	protective
Known or putative function	RcsB regulon; response envelope stress
Description	The mutants with more-pronounced lysis, and then, competition assays with serial plating confirmed their survival was significantly reduced by the TseL-active T6SS-dependent killer
Reference	[1] Hersch SJ, Watanabe N, Stietz MS, et al. Envelope stress responses defend against type six secretion system attacks independently of immunity proteins. Nat Microbiol. 2020 May;5(5):706-714. doi: 10.1038/s41564-020-0672-6. Epub 2020 Feb 24. PMID: 32094588 [2] Hersch SJ, Manera K, Dong TG. Defending against the Type Six Secretion System: beyond Immunity Genes. Cell Rep. 2020 Oct 13;33(2):108259. doi: 10.1016/j.celrep.2020.108259. PMID: 33053336

Experimental investigation has been performed.

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External database links

Locus tag (Gene)	BW25113_RS00985 (RcsF)
Coordinates (Strand)	216078..216482 (-)
NCBI ID	447125073
RefSeq	NZ_CP009273
Uniprot ID	A0A0E0Y3K7_ECO1C, K4XJM0_ECOLX, S1NN71_ECOLX, L3BWG4_ECOLX, S1EKN7_ECOLX, S1ILL5_ECOLX, L4JG83_ECOLX, A0A0E2L9W9_ECOU3, A0A0A0F9D2_ECOLX, RCSF_ECOL6, D3GRE2_ECO44, Q32JR1_SHIDS, E3PDE9_ECOH1, A7ZHT8_ECO24, A0A0E1SXR7_ECOLX, Q1RFZ2_ECOUT, A0A0H2YVY1_ECOK1, B6HZR5_ECOSE, A0A140NBJ5_ECOBD, J7QXD6_ECOLX, D6IGU0_ECOLX, D6J6J0_ECOLX, F4NQY8_ECOLX, E0IYI5_ECOLW, A0A181WTU9_KLEOX, B7UJ98_ECO27, B7MBH8_ECO45, B7LHA6_ECO55, B7N864_ECOLU, A0A0H3MEQ4_ECO7I, B7MP57_ECO81, A0A1Q8MK30_SHIBO, A0A2S8DES2_SHIDY, A0A1X3JN70_ECOLX, A0A1X3LBM0_ECOLX, A0A1X3KP04_ECOLX, A0A1X3KU34_ECOLX, F4SFK1_ECOLX, A0A1X3ID71_ECOLX, A0A1X3L9C4_ECOLX, A0A1X3M3C8_ECOLX, A0A1X3J832_ECOLX, A0A0H3EF59_ECO8N, A0A0E1LV93_ECOLX, I4T663_ECOLX, RCSF_ECOLI, A0A192CJ28_ECOLX, W8ZEJ1_ECOLX
KEGG ID	ect:ECIAI39_0447, ecy:ECSE_0198, ecz:ECS88_0208, eci:UTI89_C0212, ecq:ECED1_0203, elw:ECW_m0193, sdy:SDY_0215, ecc:c0237, ecw:EcE24377A_0203, eum:ECUMN_0194, ecv:APECO1_1791, eco:b0196, elh:ETEC_0192, ecg:E2348C_0202, ecj:JW0192, ebd:ECBD_3422, eln:NRG857_01005, ecos:EC958_0343, esl:O3K_20575, elo:EC042_0195, eck:EC55989_0194
PDB ID	6T1W, 2Y1B, 2L8Y

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Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
RcsF	PF16358.7	1.9e-51	27..132

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Transmembrane helices

• Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-134	MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAPRATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKANAVLLHSCEVTSGTPGCYRQAVCIGSALNITAK

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Signal peptides

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9992	pos: 15-16. LSG-CS.	MRALPICLVALMLSG

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  Protein sequence: 135 a.a.    .Download


  Nucleotide sequence: 405 bp    .Download