Detailed information of regulator


Summary

Regulator ID REG0336 experimental
Regulator type protein
Regulatory pathway (from literatures) TCS-RR
KEGG pathway (determined by BlastKOALA) Two-component system (map02020)
Related T6SS T6SS01291 experimental (Type i3)
Strain Salmonella enterica subsp. enterica serovar Typhimurium strain ATCC 14028
Replicon chromosome
Sequence Protein sequence (921 a.a.); Nucleotide sequence (2763 bp)
Description Real-time PCR showed a fivefold increase in sciS/TssM transcription in an ssrB mutant when compared to wild type at 5 h postinfection, confirming that SsrB negatively regulates sciS/TssM.
Reference
External database links

Locus tag (Gene) BWP10_01550 (ssrB)
Coordinates (Strand) 296600..299362 (+)
NCBI ID 1145677935
GenBank MTFW01000001
Uniprot ID A0A0C5PEN5_SALTM
KEGG ID seni:CY43_07085
KEGG pathway (determined by BlastKOALA) map02020
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
sciS/TssM transcription ↑ -
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
HATPase_c PF02518.28 5.7e-26 507..613
Response_reg PF00072.26 5e-20 691..803
HisKA PF00512.27 1.3e-14 400..460
HAMP PF00672.27 1.6e-07 314..365
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-19 MNLLNLKNTLQTSLVIRLT
Transmembrane helix 20-42 FLFLLTTIIIWLLSVLTAAYISM
Outside 43-920 VQKRQHIIEDLSVLSEMNIVLSNQRFEEAERDAKNLMYQCSLATEIHHNDIFPEVSRHLSVGPSNCTPTLNGEKHRLFLQSSDIDENSFRRDSFILNHKNEISLLSTDNPSDYSTLQPLTRKSFPLYPTHAGFYWSEPEYINGKGWHASVAVADQQGVFFEVTVKLPDLITKSHLPLDDSIRVWLDQNNHLLPFSYIPQKIRTQLENVTLHDGWQQIPGFLILRTTLHGPGWSLVTLYPYGNLHNRILKIILQQIPFTLTALVLMTSAFCWLLHRSLAKPLWRFVDVINKTATAPLSTRLPAQRLDELDSIAGAFNQLLDTLQVQYDNLENKVAERTQALNEAKKRAERANKRKSIHLTVISHELRTPMNGVLGAIELLQTTPLNIEQQGLADTARNCTLSLLAIINNLLDFSRIESGHFTLHMEETALLPLLDQAMQTIQGPAQSKKLSLRTFVGQHVPLYFHTDSIRLRQILVNLLGNAVKFTETGGIRLTVKRHEEQLIFLVSDSGKGIEIQQQSQIFTAFYQADTNSQGTGIGLTIASSLAKMMGGNLTLKSVPGVGTCVSLVLPLQEYQPPQPIKGTLSAPFCLHRQLACWGIRGEPPHQQNALLNAELLYFSGKLYDLAQQLILCTPNMPVINNLLPPWQLQILLVDDADINRDIIGKMLVSLGQHVTIAASSNEALTLSQQQRFDLVLIDIRMPEIDGIECVRLWHDEPNNLDPDCMFVALSASVATEDIHRCKKNGIHHYITKPVTLATLARYISIAAEYQLLRNIELQEQDPSRCSALLATDDMVINSKIFQSLDLLLADIENAVSAGEKIDQLIHTLKGCLGQIGQTELVCYVIDIENRVKMGKIIALEELTDLRQKIRMIFKNYTIT


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9963 - -


  Protein sequence of regulator: 921 a.a.    .

>REG0336 MTFW01000001:296600-299362 [Salmonella enterica subsp. enterica serovar Typhimurium]
MNLLNLKNTLQTSLVIRLTFLFLLTTIIIWLLSVLTAAYISMVQKRQHIIEDLSVLSEMNIVLSNQRFEEAERDAKNLMY
QCSLATEIHHNDIFPEVSRHLSVGPSNCTPTLNGEKHRLFLQSSDIDENSFRRDSFILNHKNEISLLSTDNPSDYSTLQP
LTRKSFPLYPTHAGFYWSEPEYINGKGWHASVAVADQQGVFFEVTVKLPDLITKSHLPLDDSIRVWLDQNNHLLPFSYIP
QKIRTQLENVTLHDGWQQIPGFLILRTTLHGPGWSLVTLYPYGNLHNRILKIILQQIPFTLTALVLMTSAFCWLLHRSLA
KPLWRFVDVINKTATAPLSTRLPAQRLDELDSIAGAFNQLLDTLQVQYDNLENKVAERTQALNEAKKRAERANKRKSIHL
TVISHELRTPMNGVLGAIELLQTTPLNIEQQGLADTARNCTLSLLAIINNLLDFSRIESGHFTLHMEETALLPLLDQAMQ
TIQGPAQSKKLSLRTFVGQHVPLYFHTDSIRLRQILVNLLGNAVKFTETGGIRLTVKRHEEQLIFLVSDSGKGIEIQQQS
QIFTAFYQADTNSQGTGIGLTIASSLAKMMGGNLTLKSVPGVGTCVSLVLPLQEYQPPQPIKGTLSAPFCLHRQLACWGI
RGEPPHQQNALLNAELLYFSGKLYDLAQQLILCTPNMPVINNLLPPWQLQILLVDDADINRDIIGKMLVSLGQHVTIAAS
SNEALTLSQQQRFDLVLIDIRMPEIDGIECVRLWHDEPNNLDPDCMFVALSASVATEDIHRCKKNGIHHYITKPVTLATL
ARYISIAAEYQLLRNIELQEQDPSRCSALLATDDMVINSKIFQSLDLLLADIENAVSAGEKIDQLIHTLKGCLGQIGQTE
LVCYVIDIENRVKMGKIIALEELTDLRQKIRMIFKNYTIT*

  Nucleotide sequence of regulator: 2763 bp    .

>REG0336 MTFW01000001:296600-299362 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGAATTTGCTCAATCTCAAGAATACGCTGCAAACATCTTTAGTAATCAGGCTAACTTTTTTATTTTTATTAACAACAAT
AATTATTTGGCTGCTATCTGTGCTTACCGCAGCTTATATATCAATGGTTCAGAAACGGCAGCATATAATAGAGGATTTAT
CCGTTCTATCCGAGATGAATATTGTACTAAGCAATCAACGGTTTGAAGAAGCTGAACGTGACGCTAAAAATTTAATGTAT
CAATGCTCATTAGCGACTGAGATTCATCATAACGATATTTTCCCTGAGGTGAGCCGGCATCTATCTGTCGGTCCTTCAAA
TTGCACGCCGACGCTAAACGGAGAGAAGCACCGTCTCTTTCTGCAGTCCTCTGATATCGATGAAAATAGCTTTCGTCGCG
ATAGTTTTATTCTTAATCATAAAAATGAGATTTCGTTATTATCTACTGATAACCCTTCAGATTATTCAACTCTACAGCCT
TTAACGCGAAAAAGCTTTCCTTTATACCCAACCCATGCCGGGTTTTACTGGAGTGAACCAGAATACATAAACGGCAAAGG
ATGGCACGCTTCCGTTGCGGTTGCCGATCAGCAAGGCGTATTTTTTGAGGTGACGGTTAAACTTCCCGATCTCATTACTA
AGAGCCACCTGCCATTAGATGATAGTATTCGAGTATGGCTGGATCAAAACAACCACTTATTGCCGTTTTCATACATCCCG
CAAAAAATACGTACACAGTTAGAAAATGTAACGCTGCATGATGGATGGCAGCAAATTCCCGGATTTCTGATATTACGCAC
AACCTTGCATGGCCCCGGATGGAGTCTGGTTACGCTGTACCCATACGGTAATCTACATAATCGCATCTTAAAAATTATCC
TTCAACAAATCCCCTTTACATTAACAGCATTGGTGTTGATGACGTCGGCTTTTTGCTGGTTACTACATCGCTCACTGGCC
AAACCGTTATGGCGTTTTGTCGATGTCATTAATAAAACCGCAACTGCACCGCTGAGCACACGTTTACCAGCACAACGACT
GGATGAATTAGATAGTATTGCCGGTGCTTTTAACCAACTGCTTGATACTCTACAAGTCCAATACGACAATCTGGAAAACA
AAGTCGCAGAGCGCACCCAGGCGCTAAATGAAGCAAAAAAACGCGCTGAGCGAGCTAACAAACGTAAAAGCATTCATCTT
ACGGTAATAAGTCATGAGTTACGTACTCCGATGAATGGCGTACTCGGTGCAATTGAATTATTACAAACCACCCCTTTAAA
CATAGAGCAACAAGGATTAGCTGATACCGCCAGAAATTGTACACTGTCTTTGTTAGCTATTATTAATAATCTGCTGGATT
TTTCACGCATCGAGTCTGGTCATTTCACATTACATATGGAAGAAACAGCGTTACTGCCGTTACTGGACCAGGCAATGCAA
ACCATCCAGGGGCCAGCGCAAAGCAAAAAACTGTCATTACGTACTTTTGTCGGTCAACATGTCCCTCTCTATTTTCATAC
CGACAGTATCCGTTTACGGCAAATTTTGGTTAATTTACTCGGGAACGCGGTAAAATTTACCGAAACCGGAGGGATACGTC
TGACGGTCAAGCGTCATGAGGAACAATTAATATTTCTGGTTAGCGATAGCGGTAAAGGGATTGAAATACAGCAGCAGTCT
CAAATCTTTACTGCTTTTTATCAAGCAGACACAAATTCGCAAGGTACAGGAATTGGACTGACTATTGCGTCAAGCCTGGC
TAAAATGATGGGCGGTAATCTGACACTAAAAAGTGTCCCCGGGGTTGGAACCTGTGTCTCGCTAGTATTACCCTTACAAG
AATACCAGCCGCCTCAACCAATTAAAGGGACGCTGTCAGCGCCGTTCTGCCTGCATCGGCAACTGGCTTGCTGGGGAATA
CGCGGTGAACCACCCCACCAGCAAAATGCGCTTCTCAACGCAGAGCTTTTGTATTTCTCCGGAAAACTCTACGACCTGGC
GCAACAGTTAATATTGTGTACACCAAATATGCCAGTAATAAATAATTTGTTACCACCCTGGCAGTTGCAGATTCTTTTGG
TTGATGATGCCGATATTAATCGGGATATCATCGGCAAAATGCTTGTCAGCCTGGGCCAACACGTCACTATTGCCGCCAGT
AGTAACGAGGCTCTGACTTTATCACAACAGCAGCGATTCGATTTAGTACTGATTGACATTAGAATGCCAGAAATAGATGG
TATTGAATGTGTACGATTATGGCATGATGAGCCGAATAATTTAGATCCTGACTGCATGTTTGTGGCACTATCCGCTAGCG
TAGCGACAGAAGATATTCATCGTTGTAAAAAAAATGGGATTCATCATTACATTACAAAACCAGTGACATTGGCTACCTTA
GCTCGCTACATCAGTATTGCCGCAGAATACCAACTTTTACGAAATATAGAGCTACAGGAGCAGGATCCGAGTCGCTGCTC
AGCGCTACTGGCGACAGATGATATGGTCATTAATAGCAAGATTTTCCAATCACTGGACCTCTTGCTGGCTGATATTGAAA
ATGCCGTATCGGCTGGAGAAAAAATCGATCAGTTAATTCACACATTAAAAGGCTGTTTAGGTCAAATAGGGCAGACTGAA
TTGGTATGCTATGTCATAGACATTGAGAATCGCGTAAAAATGGGGAAAATCATCGCGCTGGAGGAACTAACCGACTTACG
CCAGAAAATACGTATGATCTTCAAAAACTACACCATTACTTAA