Detailed information of Recipient susceptibility factor
Summary
| ID | RSF000062  |
| Strain | Escherichia coli strain MOD1-EC300 |
| Replicon | chromosome |
| Sequence | Protein sequence (163 a.a.); Nucleotide sequence (489 bp) |
| Response to T6SS attack | protective |
| Known or putative function | capsule protection |
| Description | Consistent with the encapsulation of Ec300, the WT (CPS+) was resistant to T6SS attacks, while the survival of the non- encapsulated mutant (CPS-) as well as the K-12 and MG1655 strains was significantly reduced by V. cholerae. |
| Reference |
Experimental investigation has been performed.External database links
| Locus tag (Gene) | BSJ02_RS12510 (rfaH) |
| Coordinates (Strand) | 10609..11097 (+) |
| NCBI ID | WP_096838056.1 |
| RefSeq | NZ_NJMM01000018 |
| Uniprot ID | UPI000BDF78CC |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| NusG | PF02357.21 | 8.1e-18 | 3..95 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-162 | MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRTAVSESLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPKDIVDPATPYPGDKVIITEGTFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFRKL |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9889 | - | - |
Protein sequence: 163 a.a. .
>RSF000062 NZ_NJMM01000018:10609-11097 [Escherichia coli]
MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRTAVSESLFPNYLFVEFDPEVIHTTTINATRGVSHFVR
FGASPAIVPSAVIHQLSVYKPKDIVDPATPYPGDKVIITEGTFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
KL*
MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRTAVSESLFPNYLFVEFDPEVIHTTTINATRGVSHFVR
FGASPAIVPSAVIHQLSVYKPKDIVDPATPYPGDKVIITEGTFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
KL*
Nucleotide sequence: 489 bp .
>RSF000062 NZ_NJMM01000018:10609-11097 [Escherichia coli]
ATGCAATCCTGGTATTTACTGTACTGCAAGCGCGGGCAACTTCAACGTGCCCAGGAACACCTCGAAAGACAGGCTGTGAA
TTGCCTGGCACCGATGATCACCCTGGAAAAAATCGTGCGTGGAAAACGTACTGCAGTCAGTGAGTCATTGTTCCCCAACT
ACCTGTTTGTGGAATTTGATCCAGAAGTGATTCATACCACGACTATCAACGCGACCCGCGGTGTCAGCCACTTCGTGCGC
TTTGGCGCGTCGCCAGCGATAGTCCCATCGGCGGTTATTCATCAGCTATCGGTATATAAACCGAAAGACATTGTCGATCC
GGCAACCCCTTATCCGGGTGATAAGGTGATTATTACCGAAGGCACGTTCGAAGGCTTTCAGGCCATTTTCACCGAACCCG
ATGGTGAGGCTCGCTCCATGCTATTGCTTAATCTTATTAATAAAGAGATTAAGCACAGTGTGAAGAATACCGAGTTCCGC
AAACTCTAA
ATGCAATCCTGGTATTTACTGTACTGCAAGCGCGGGCAACTTCAACGTGCCCAGGAACACCTCGAAAGACAGGCTGTGAA
TTGCCTGGCACCGATGATCACCCTGGAAAAAATCGTGCGTGGAAAACGTACTGCAGTCAGTGAGTCATTGTTCCCCAACT
ACCTGTTTGTGGAATTTGATCCAGAAGTGATTCATACCACGACTATCAACGCGACCCGCGGTGTCAGCCACTTCGTGCGC
TTTGGCGCGTCGCCAGCGATAGTCCCATCGGCGGTTATTCATCAGCTATCGGTATATAAACCGAAAGACATTGTCGATCC
GGCAACCCCTTATCCGGGTGATAAGGTGATTATTACCGAAGGCACGTTCGAAGGCTTTCAGGCCATTTTCACCGAACCCG
ATGGTGAGGCTCGCTCCATGCTATTGCTTAATCTTATTAATAAAGAGATTAAGCACAGTGTGAAGAATACCGAGTTCCGC
AAACTCTAA