Detailed information of effector
Summary
| Effector ID | EFF01309  |
| Effector type | - |
| Cognate immunity protein | - |
| Related T6SS (Type) | T6SS00904 (Type i2) |
| Strain | Escherichia coli strain TW-XM |
| Replicon | chromosome |
| Sequence | Protein sequence (164 a.a.); Nucleotide sequence (492 bp) |
| Description | Hcp1 was assembled in the T6SS complexes and secreted into the extracellular matrix to activate humoral immunity during duck infection. |
| Reference | [1] Ma J, Bao Y, Sun M, et al. Two functional type VI secretion systems in avian pathogenic Escherichia coli are involved in different pathogenic pathways. Infect Immun. 2014 Sep;82(9):3867-79. doi: 10.1128/IAI.01769-14. Epub 2014 Jun 30. PMID: 24980972 [2] Ma J, Sun M, Pan Z, et al. Three Hcp homologs with divergent extended loop regions exhibit different functions in avian pathogenic Escherichia coli. Emerg Microbes Infect. 2018 Mar 29;7(1):49. doi: 10.1038/s41426-018-0042-0. PMID: 29593238 |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | xmoF (Hcp1) |
| Coordinates (Strand) | 5838..6329 (+) |
| NCBI ID | 611963684 |
| GenBank | KF678349 |
| Uniprot ID | X5FUJ5_ECOLX |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| T6SS_HCP | PF05638.14 | 3.5e-38 | 6..146 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-163 | MAIPAYLWLKDDGGADIKGSVDVQGREGSIEVVALDHDVYIPTDNNTGKLTGTRTHKPFTFTKEIDASSPYLYKAVTTGQTLKTAEFKFYRINDAGQEVEYFNTTLDNVKLVRVAPLMHDIKDPGKEKHNHLERIEFRYEKITWTYKDGNIIHSDSWNERPSA |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9853 | - | - |
Protein sequence: 164 a.a. .
>EFF01309 KF678349:5838-6329 (Unknown) [Escherichia coli]
MAIPAYLWLKDDGGADIKGSVDVQGREGSIEVVALDHDVYIPTDNNTGKLTGTRTHKPFTFTKEIDASSPYLYKAVTTGQ
TLKTAEFKFYRINDAGQEVEYFNTTLDNVKLVRVAPLMHDIKDPGKEKHNHLERIEFRYEKITWTYKDGNIIHSDSWNER
PSA*
MAIPAYLWLKDDGGADIKGSVDVQGREGSIEVVALDHDVYIPTDNNTGKLTGTRTHKPFTFTKEIDASSPYLYKAVTTGQ
TLKTAEFKFYRINDAGQEVEYFNTTLDNVKLVRVAPLMHDIKDPGKEKHNHLERIEFRYEKITWTYKDGNIIHSDSWNER
PSA*
Nucleotide sequence: 492 bp .
>EFF01309 KF678349:5838-6329 (Unknown) [Escherichia coli]
ATGGCTATTCCTGCTTATCTCTGGCTGAAAGATGACGGCGGCGCGGATATCAAAGGTTCCGTGGACGTTCAGGGGCGCGA
AGGTAGCATCGAAGTGGTGGCGCTGGATCACGATGTGTACATCCCGACCGACAATAACACCGGCAAACTGACCGGTACCC
GTACTCACAAGCCTTTTACGTTTACCAAAGAAATCGATGCCTCCAGCCCGTATCTCTACAAAGCTGTGACCACCGGACAG
ACCCTGAAAACGGCGGAGTTTAAATTCTACCGCATCAACGATGCCGGTCAGGAAGTGGAGTATTTCAATACCACGCTGGA
TAACGTCAAGCTGGTCAGAGTAGCTCCGCTGATGCACGACATCAAAGATCCGGGTAAAGAGAAGCATAACCACCTGGAAC
GCATTGAGTTCCGCTACGAGAAAATCACCTGGACTTACAAAGACGGCAACATCATTCATTCCGACTCGTGGAATGAGCGT
CCTTCCGCCTGA
ATGGCTATTCCTGCTTATCTCTGGCTGAAAGATGACGGCGGCGCGGATATCAAAGGTTCCGTGGACGTTCAGGGGCGCGA
AGGTAGCATCGAAGTGGTGGCGCTGGATCACGATGTGTACATCCCGACCGACAATAACACCGGCAAACTGACCGGTACCC
GTACTCACAAGCCTTTTACGTTTACCAAAGAAATCGATGCCTCCAGCCCGTATCTCTACAAAGCTGTGACCACCGGACAG
ACCCTGAAAACGGCGGAGTTTAAATTCTACCGCATCAACGATGCCGGTCAGGAAGTGGAGTATTTCAATACCACGCTGGA
TAACGTCAAGCTGGTCAGAGTAGCTCCGCTGATGCACGACATCAAAGATCCGGGTAAAGAGAAGCATAACCACCTGGAAC
GCATTGAGTTCCGCTACGAGAAAATCACCTGGACTTACAAAGACGGCAACATCATTCATTCCGACTCGTGGAATGAGCGT
CCTTCCGCCTGA