Detailed information of regulator


Summary

Regulator ID REG0272 experimental
Regulator type protein
Regulatory pathway (from literatures) Global regulator
KEGG pathway (determined by BlastKOALA) Mismatch repair (map03430)
Related T6SS T6SS00931 experimental (Type i4b)
Strain Escherichia coli strain 17-2
Replicon chromosome
Sequence Protein sequence (279 a.a.); Nucleotide sequence (837 bp)
Description Fur binding prevents DNA adenine methylation at the GATC-I site and methylation of the fur1 box had a negative impact on Fur binding.
Reference
External database links

Locus tag (Gene) H1D38_12840 (Dam)
Coordinates (Strand) 105877..106713 (+)
NCBI ID MBA2234741.1
GenBank JACEFV010000002
Uniprot ID -
KEGG ID -
KEGG pathway (determined by BlastKOALA) map03430
PDB ID -
  Target
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Target
Regulation (↓/↑)
Target sequence
sciH promotor↑TTCCATAGAATAAAGTCCTGATTATTTGCATTATATCGATCGATGTATCTGTTATATTGAGATTTTTCAGATCTTCGTCCTATAATGATCAAATTAAA The red sequences indicate Fur binding boxes and GATC Dam methylation sites are indicated in bold blue letters
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Pfam domain hit(s) of regulator

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Domain
Pfam ID
E-value
Aligned region
MethyltransfD12PF02086.171.9e-7510..251
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Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

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Prediction                
Region    
Sequence
Outside1-278MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLNTDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQDPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFFYCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVERHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPAKK


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
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Prediction       
Probability
Cleavage site
Signal peptide sequence
Other0.9945--


  Protein sequence of regulator: 279 a.a.    .

>REG0272 JACEFV010000002:105877-106713 [Escherichia coli]
MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLNTDFSRYILADINSDLISLYNIVKMRTDEYVQAAREL
FVPETNCAEVYYQFREEFNKSQDPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVERHIPVLISNHDTMLTREWYQRA
KLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPAKK*

  Nucleotide sequence of regulator: 837 bp    .

>REG0272 JACEFV010000002:105877-106713 [Escherichia coli]
ATGAAGAAAAATCGCGCTTTTTTGAAGTGGGCAGGGGGCAAGTATCCCCTGCTTGATGATATTAAACGGCATTTGCCCAA
GGGCGAATGTCTGGTTGAGCCTTTTGTAGGTGCCGGGTCGGTGTTTCTCAACACCGACTTTTCTCGTTATATCCTTGCCG
ATATCAATAGCGACCTGATCAGTCTCTATAACATTGTGAAGATGCGTACTGATGAGTACGTACAGGCCGCACGCGAGCTG
TTTGTTCCCGAAACAAATTGCGCCGAGGTTTACTATCAGTTCCGCGAAGAGTTCAACAAAAGCCAGGATCCGTTCCGTCG
GGCGGTACTGTTTTTATATTTGAACCGCTACGGTTACAACGGCCTGTGTCGTTACAATCTGCGCGGTGAGTTTAACGTGC
CGTTCGGCCGCTACAAAAAACCCTATTTCCCGGAAGCAGAGTTGTATCACTTCGCTGAAAAAGCGCAGAATGCCTTTTTC
TATTGTGAGTCTTACGCCGATAGCATGGCGCGCGCAGATGATGCATCCGTCGTCTATTGCGATCCGCCTTATGCACCGCT
GTCTGCGACCGCCAACTTTACGGCGTATCACACAAACAGTTTTACGCTTGAACAACAAGCGCATCTGGCGGAGATCGCCG
AAGGTCTGGTTGAGCGCCATATTCCAGTGCTGATCTCCAATCACGATACGATGTTAACGCGTGAGTGGTATCAGCGCGCA
AAATTGCATGTCGTCAAAGTTCGACGCAGTATAAGCAGCAACGGCGGCACACGTAAAAAGGTGGACGAACTGCTGGCTTT
GTACAAACCAGGAGTCGTTTCACCCGCGAAAAAATAA