Detailed information of T6SS

Note: only experimentally validated proteins are displayed in the graph.


Summary

T6SS ID T6SS00931 experimental
T6SS type Type i4b
Strain Escherichia coli 17-2
Replicon (RefSeq) chromosome (NZ_JACEFV010000001)
Location 883479..901846
Function anti-eukaryotic; effector translocation
Comment sci-1 gene cluster is involved in biofilm formation.
References
[1] Aschtgen MS et al (2008) SciN is an outer membrane lipoprotein required for type VI secretion in enteroaggregative Escherichia coli. J Bacteriol. 190(22):7523-31. [PudMed:18805985]
[2] Zoued A et al (2013) TssK is a trimeric cytoplasmic protein interacting with components of both phage-like and membrane anchoring complexes of the Type VI secretion system. J Biol Chem. 288(38):27031-41. [PudMed:23921384]
[3] Nguyen VS et al (2015) Inhibition of Type VI Secretion by an Anti-TssM Llama Nanobody. PLoS One. 10(3):e0122187. [PudMed:25811612]
[4] Durand E et al (2012) Structural characterization and oligomerization of the TssL protein, a component shared by bacterial type VI and type IVb secretion systems. J Biol Chem. 287(17):14157-68. [PudMed:22371492]
[5] Aschtgen MS et al (2012) The C-tail anchored TssL subunit, an essential protein of the enteroaggregative Escherichia coli Sci-1 Type VI secretion system, is inserted by YidC. Microbiologyopen. 1(1):71-82. [PudMed:22950014]
[6] Felisberto-Rodrigues C et al (2011) Towards a structural comprehension of bacterial type VI secretion systems: characterization of the TssJ-TssM complex of an Escherichia coli pathovar. PLoS Pathog. 7(11):e1002386. [PudMed:22102820]
[7] Aschtgen MS et al (2010) The SciZ protein anchors the enteroaggregative Escherichia coli Type VI secretion system to the cell wall. Mol Microbiol. 75(4):886-99. [PudMed:20487285]
[8] Brunet YR et al (2013) Imaging Type VI Secretion-Mediated Bacterial Killing. Cell Rep. 3(1):36-41. [PudMed:23291094]
experimental Experimental investigation has been performed.

T6SS components and genome coordinates

ComponentTssATssBTssCTssDTssFTssGTssHTssITssJTssKTssLTssM
Number111111111111


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Component ID
Component
Locus tag (Gene)
Coordinate
Strand
Size (bp)
NCBI ID
Product
H1D38_RS04205879397..879677-281pseudogenetransposase
H1D38_RS04210879742..880308-567446510373hypothetical protein
H1D38_RS04215882071..883282-1212pseudogeneIS3 family transposase
T6CP016057TssHH1D38_RS04220 (tssH)883479..886016-25382013783413type VI secretion system ATPase TssH
T6CP016058TssMH1D38_RS04225886032..889436-3405485799630type VI secretion protein
T6CP016059TssLH1D38_RS04230889442..890011-5701774265096DotU family type IV/VI secretion system protein
T6CP016060TssKH1D38_RS04235 (tssK)890371..891783-1413447077409type VI secretion system baseplate subunit TssK
T6CP016061TssJH1D38_RS04240891802..892353-552446074892type VI secretion lipoprotein TssJ
T6CP016062TssAH1D38_RS04245 (tssA)892361..893437-1077446955899type VI secretion system protein TssA
H1D38_RS04250893441..893740-300485765576PAAR domain-containing protein
Showing 1 to 10 of 26 rows rows per page
flank Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files

    

Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
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Regulator ID
Locus tag (Gene)
Regulator type
Regulatory pathway
Organism                
Replicon (Accession)
Coordinates (Strand)
NCBI ID
Target (Regulation)
REG0271 experimentalH1D38_08255 (fur)proteinGlobal regulatorEscherichia coli strain 17-2chromosome (JACEFV010000001)1744748..1745194 (-)MBA2233871.1sciH promotor (↑)
REG0272 experimentalH1D38_12840 (Dam)proteinGlobal regulatorEscherichia coli strain 17-2chromosome (JACEFV010000002)105877..106713 (+)MBA2234741.1sciH promotor (↑)
Showing 1 to 2 of 2 rows

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
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Effector ID
Locus tag (Gene)
Classification
Cognate immunity protein
Organism                
Replicon (Accession)
Coordinates (Strand)
NCBI ID
EFF01465 experimentalEC042_RS24220TleIMU00313 experimentalEscherichia coli 042chromosome (NC_017626)4865605..4867287 (+)284924255
EFF01498 experimental-TpeIMU00327 experimentalEscherichia coli strain TW-XMchromosome (KF678350)24197..25636 (+)611963723
Showing 1 to 2 of 2 rows

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
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Immunity protein ID
Locus tag (Gene)
Cognate effector
Organism                
Replicon (Accession)
Coordinates (Strand)
NCBI ID
No matching records found

experimental Experimental investigation has been performed.
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Accessory protein ID
Locus tag (Gene)
Type            
Organism                
Replicon (Accession)
Coordinates (Strand)
NCBI ID
Related structure protein
Related effector
ACP00032 experimentalEC042_RS24300 (tagA)structure affiliated proteinEscherichia coli 042chromosome (NC_017626)4884070..4885437 (+)446122356TssA-
ACP00033 experimentalEC042_RS24190 (tagL/ompA)structure affiliated proteinEscherichia coli 042chromosome (NC_017626)4856944..4858674 (+)446480629TssLpeptidoglycan
ACP00034 experimentalEC042_1244 (mltE)structure affiliated proteinEscherichia coli 042chromosome (NC_017626)1322321..1322932 (+)481024453TssM-
ACP00035 experimentalEC042_RS24190 (sciZ)structure affiliated proteinEscherichia coli 042chromosome (NC_017626)4856944..4858674 (+)446480629SciP
ACP00036 experimentalEC042_RS21585 (yidC)chaperoneEscherichia coli 042chromosome (NC_017626)4320019..4321665 (+)446300403TssL-
ACP00037 experimentalEC042_RS00085 (dnaK)chaperoneEscherichia coli 042chromosome (NC_017626)13600..15516 (+)446438280TssL-
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