Detailed information of T6SS
Note: only experimentally validated proteins are displayed in the graph.
Summary
T6SS ID | T6SS00931 |
T6SS type | Type i4b |
Strain | Escherichia coli 17-2 |
Replicon (RefSeq) | chromosome (NZ_JACEFV010000001) |
Location | 883479..901846 |
Function | anti-eukaryotic; effector translocation |
Comment | sci-1 gene cluster is involved in biofilm formation. |
References | [1] Aschtgen MS et al (2008) SciN is an outer membrane lipoprotein required for type VI secretion in enteroaggregative Escherichia coli. J Bacteriol. 190(22):7523-31. [PudMed:18805985] [2] Zoued A et al (2013) TssK is a trimeric cytoplasmic protein interacting with components of both phage-like and membrane anchoring complexes of the Type VI secretion system. J Biol Chem. 288(38):27031-41. [PudMed:23921384] [3] Nguyen VS et al (2015) Inhibition of Type VI Secretion by an Anti-TssM Llama Nanobody. PLoS One. 10(3):e0122187. [PudMed:25811612] [4] Durand E et al (2012) Structural characterization and oligomerization of the TssL protein, a component shared by bacterial type VI and type IVb secretion systems. J Biol Chem. 287(17):14157-68. [PudMed:22371492] [5] Aschtgen MS et al (2012) The C-tail anchored TssL subunit, an essential protein of the enteroaggregative Escherichia coli Sci-1 Type VI secretion system, is inserted by YidC. Microbiologyopen. 1(1):71-82. [PudMed:22950014] [6] Felisberto-Rodrigues C et al (2011) Towards a structural comprehension of bacterial type VI secretion systems: characterization of the TssJ-TssM complex of an Escherichia coli pathovar. PLoS Pathog. 7(11):e1002386. [PudMed:22102820] [7] Aschtgen MS et al (2010) The SciZ protein anchors the enteroaggregative Escherichia coli Type VI secretion system to the cell wall. Mol Microbiol. 75(4):886-99. [PudMed:20487285] [8] Brunet YR et al (2013) Imaging Type VI Secretion-Mediated Bacterial Killing. Cell Rep. 3(1):36-41. [PudMed:23291094] |
T6SS components and genome coordinates
Component | TssA | TssB | TssC | TssD | TssF | TssG | TssH | TssI | TssJ | TssK | TssL | TssM |
Number | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
Component ID | Component | Locus tag (Gene) | Coordinate | Strand | Size (bp) | NCBI ID | Product |
---|---|---|---|---|---|---|---|
H1D38_RS04205 | 879397..879677 | - | 281 | pseudogene | transposase | ||
H1D38_RS04210 | 879742..880308 | - | 567 | 446510373 | hypothetical protein | ||
H1D38_RS04215 | 882071..883282 | - | 1212 | pseudogene | IS3 family transposase | ||
T6CP016057 | TssH | H1D38_RS04220 (tssH) | 883479..886016 | - | 2538 | 2013783413 | type VI secretion system ATPase TssH |
T6CP016058 | TssM | H1D38_RS04225 | 886032..889436 | - | 3405 | 485799630 | type VI secretion protein |
T6CP016059 | TssL | H1D38_RS04230 | 889442..890011 | - | 570 | 1774265096 | DotU family type IV/VI secretion system protein |
T6CP016060 | TssK | H1D38_RS04235 (tssK) | 890371..891783 | - | 1413 | 447077409 | type VI secretion system baseplate subunit TssK |
T6CP016061 | TssJ | H1D38_RS04240 | 891802..892353 | - | 552 | 446074892 | type VI secretion lipoprotein TssJ |
T6CP016062 | TssA | H1D38_RS04245 (tssA) | 892361..893437 | - | 1077 | 446955899 | type VI secretion system protein TssA |
H1D38_RS04250 | 893441..893740 | - | 300 | 485765576 | PAAR domain-containing protein | ||
H1D38_RS04255 | 893750..894217 | - | 468 | 445927225 | hypothetical protein | ||
T6CP016063 | TssI | H1D38_RS04260 (tssI) | 894228..896210 | - | 1983 | 446120415 | type VI secretion system tip protein VgrG |
T6CP016064 | TssG | H1D38_RS04265 (tssG) | 896223..897155 | - | 933 | 1883827381 | type VI secretion system baseplate subunit TssG |
T6CP016065 | TssF | H1D38_RS04270 (tssF) | 897146..898948 | - | 1803 | 485765577 | type VI secretion system baseplate subunit TssF |
H1D38_RS04275 | 898941..899363 | - | 423 | 446477695 | hypothetical protein | ||
T6CP016066 | TssD | H1D38_RS04280 (aaiC) | 899373..899879 | - | 507 | 446155335 | type VI secretion system protein AaiC/Hcp2 |
T6CP016067 | TssC | H1D38_RS04285 (tssC) | 899889..901367 | - | 1479 | 446137198 | type VI secretion system contractile sheath large subunit |
T6CP016068 | TssB | H1D38_RS04290 (tssB) | 901370..901846 | - | 477 | 445994694 | type VI secretion system contractile sheath small subunit |
H1D38_RS04295 | 902238..903254 | + | 1017 | pseudogene | IS630 family transposase | ||
H1D38_RS04300 | 903260..903385 | - | 126 | 485799638 | hypothetical protein | ||
H1D38_RS04305 | 903363..903686 | - | 324 | 446181679 | hypothetical protein | ||
H1D38_RS04310 | 903959..904538 | + | 580 | pseudogene | transposase family protein | ||
H1D38_RS04315 | 904569..904892 | - | 324 | 485761811 | hypothetical protein | ||
H1D38_RS04320 | 904958..905602 | - | 645 | 485761812 | hypothetical protein | ||
H1D38_RS04325 | 905623..905892 | - | 270 | 446148662 | PerC family transcriptional regulator | ||
H1D38_RS04330 | 905971..906615 | - | 645 | 446425008 | ParB N-terminal domain-containing protein |
Download FASTA format files
Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
Experimental investigation has been performed.
Bioinformatics investigation has been performed.
Regulator ID | Locus tag (Gene) | Regulator type | Regulatory pathway | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Target (Regulation) |
---|---|---|---|---|---|---|---|---|
REG0271 | H1D38_08255 (fur) | protein | Global regulator | Escherichia coli strain 17-2 | chromosome (JACEFV010000001) | 1744748..1745194 (-) | MBA2233871.1 | sciH promotor (↑) |
REG0272 | H1D38_12840 (Dam) | protein | Global regulator | Escherichia coli strain 17-2 | chromosome (JACEFV010000002) | 105877..106713 (+) | MBA2234741.1 | sciH promotor (↑) |
Experimental investigation has been performed.
Bioinformatics investigation has been performed.
Effector ID | Locus tag (Gene) | Classification | Cognate immunity protein | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
---|---|---|---|---|---|---|---|
EFF01465 | EC042_RS24220 | Tle | IMU00313 | Escherichia coli 042 | chromosome (NC_017626) | 4865605..4867287 (+) | 284924255 |
EFF01498 | - | Tpe | IMU00327 | Escherichia coli strain TW-XM | chromosome (KF678350) | 24197..25636 (+) | 611963723 |
Experimental investigation has been performed.
Bioinformatics investigation has been performed.
Immunity protein ID | Locus tag (Gene) | Cognate effector | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
---|
Experimental investigation has been performed.
Accessory protein ID | Locus tag (Gene) | Type | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Related structure protein | Related effector |
---|---|---|---|---|---|---|---|---|
ACP00032 | EC042_RS24300 (tagA) | structure affiliated protein | Escherichia coli 042 | chromosome (NC_017626) | 4884070..4885437 (+) | 446122356 | TssA | - |
ACP00033 | EC042_RS24190 (tagL/ompA) | structure affiliated protein | Escherichia coli 042 | chromosome (NC_017626) | 4856944..4858674 (+) | 446480629 | TssL | peptidoglycan |
ACP00034 | EC042_1244 (mltE) | structure affiliated protein | Escherichia coli 042 | chromosome (NC_017626) | 1322321..1322932 (+) | 481024453 | TssM | - |
ACP00035 | EC042_RS24190 (sciZ) | structure affiliated protein | Escherichia coli 042 | chromosome (NC_017626) | 4856944..4858674 (+) | 446480629 | SciP | |
ACP00036 | EC042_RS21585 (yidC) | chaperone | Escherichia coli 042 | chromosome (NC_017626) | 4320019..4321665 (+) | 446300403 | TssL | - |
ACP00037 | EC042_RS00085 (dnaK) | chaperone | Escherichia coli 042 | chromosome (NC_017626) | 13600..15516 (+) | 446438280 | TssL | - |