Detailed information of T6SS

Note: only experimentally validated proteins are displayed in the graph.


Summary

T6SS ID T6SS00931 experimental
T6SS type Type i4b
Strain Escherichia coli 17-2
Replicon (RefSeq) chromosome (NZ_JACEFV010000001)
Location 883479..901846
Function anti-eukaryotic; effector translocation
Comment sci-1 gene cluster is involved in biofilm formation.
References
[1] Aschtgen MS et al (2008) SciN is an outer membrane lipoprotein required for type VI secretion in enteroaggregative Escherichia coli. J Bacteriol. 190(22):7523-31. [PudMed:18805985]
[2] Aschtgen MS et al (2010) The SciZ protein anchors the enteroaggregative Escherichia coli Type VI secretion system to the cell wall. Mol Microbiol. 75(4):886-99. [PudMed:20487285]
[3] Felisberto-Rodrigues C et al (2011) Towards a structural comprehension of bacterial type VI secretion systems: characterization of the TssJ-TssM complex of an Escherichia coli pathovar. PLoS Pathog. 7(11):e1002386. [PudMed:22102820]
[4] Durand E et al (2012) Structural characterization and oligomerization of the TssL protein, a component shared by bacterial type VI and type IVb secretion systems. J Biol Chem. 287(17):14157-68. [PudMed:22371492]
[5] Aschtgen MS et al (2012) The C-tail anchored TssL subunit, an essential protein of the enteroaggregative Escherichia coli Sci-1 Type VI secretion system, is inserted by YidC. Microbiologyopen. 1(1):71-82. [PudMed:22950014]
[6] Zoued A et al (2013) TssK is a trimeric cytoplasmic protein interacting with components of both phage-like and membrane anchoring complexes of the Type VI secretion system. J Biol Chem. 288(38):27031-41. [PudMed:23921384]
[7] Brunet YR et al (2013) Imaging Type VI Secretion-Mediated Bacterial Killing. Cell Rep. 3(1):36-41. [PudMed:23291094]
[8] Nguyen VS et al (2015) Inhibition of Type VI Secretion by an Anti-TssM Llama Nanobody. PLoS One. 10(3):e0122187. [PudMed:25811612]
experimental Experimental investigation has been performed.

T6SS components and genome coordinates

ComponentTssATssBTssCTssDTssFTssGTssHTssITssJTssKTssLTssM
Number111111111111


Component ID Component Locus tag (Gene) Coordinate Strand Size (bp) NCBI ID Product
H1D38_RS04205 879397..879677 - 281 pseudogene transposase
H1D38_RS04210 879742..880308 - 567 446510373 hypothetical protein
H1D38_RS04215 882071..883282 - 1212 pseudogene IS3 family transposase
T6CP016057 TssH H1D38_RS04220 (tssH) 883479..886016 - 2538 2013783413 type VI secretion system ATPase TssH
T6CP016058 TssM H1D38_RS04225 886032..889436 - 3405 485799630 type VI secretion protein
T6CP016059 TssL H1D38_RS04230 889442..890011 - 570 1774265096 DotU family type IV/VI secretion system protein
T6CP016060 TssK H1D38_RS04235 (tssK) 890371..891783 - 1413 447077409 type VI secretion system baseplate subunit TssK
T6CP016061 TssJ H1D38_RS04240 891802..892353 - 552 446074892 type VI secretion lipoprotein TssJ
T6CP016062 TssA H1D38_RS04245 (tssA) 892361..893437 - 1077 446955899 type VI secretion system protein TssA
H1D38_RS04250 893441..893740 - 300 485765576 PAAR domain-containing protein
H1D38_RS04255 893750..894217 - 468 445927225 hypothetical protein
T6CP016063 TssI H1D38_RS04260 (tssI) 894228..896210 - 1983 446120415 type VI secretion system tip protein VgrG
T6CP016064 TssG H1D38_RS04265 (tssG) 896223..897155 - 933 1883827381 type VI secretion system baseplate subunit TssG
T6CP016065 TssF H1D38_RS04270 (tssF) 897146..898948 - 1803 485765577 type VI secretion system baseplate subunit TssF
H1D38_RS04275 898941..899363 - 423 446477695 hypothetical protein
T6CP016066 TssD H1D38_RS04280 (aaiC) 899373..899879 - 507 446155335 type VI secretion system protein AaiC/Hcp2
T6CP016067 TssC H1D38_RS04285 (tssC) 899889..901367 - 1479 446137198 type VI secretion system contractile sheath large subunit
T6CP016068 TssB H1D38_RS04290 (tssB) 901370..901846 - 477 445994694 type VI secretion system contractile sheath small subunit
H1D38_RS04295 902238..903254 + 1017 pseudogene IS630 family transposase
H1D38_RS04300 903260..903385 - 126 485799638 hypothetical protein
H1D38_RS04305 903363..903686 - 324 446181679 hypothetical protein
H1D38_RS04310 903959..904538 + 580 pseudogene transposase family protein
H1D38_RS04315 904569..904892 - 324 485761811 hypothetical protein
H1D38_RS04320 904958..905602 - 645 485761812 hypothetical protein
H1D38_RS04325 905623..905892 - 270 446148662 PerC family transcriptional regulator
H1D38_RS04330 905971..906615 - 645 446425008 ParB N-terminal domain-containing protein
flank Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files

    

Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Regulator ID Locus tag (Gene) Regulator type Regulatory pathway Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Target (Regulation)
REG0271 experimental H1D38_08255 (fur) protein Global regulator Escherichia coli strain 17-2 chromosome (JACEFV010000001) 1744748..1745194 (-) MBA2233871.1 sciH promotor (↑)
REG0272 experimental H1D38_12840 (Dam) protein Global regulator Escherichia coli strain 17-2 chromosome (JACEFV010000002) 105877..106713 (+) MBA2234741.1 sciH promotor (↑)

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Effector ID Locus tag (Gene) Classification Cognate immunity protein Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID
EFF01465 experimental EC042_RS24220 Tle IMU00313 experimental Escherichia coli 042 chromosome (NC_017626) 4865605..4867287 (+) 284924255
EFF01498 experimental - Tpe IMU00327 experimental Escherichia coli strain TW-XM chromosome (KF678350) 24197..25636 (+) 611963723

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Immunity protein ID Locus tag (Gene) Cognate effector Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID

experimental Experimental investigation has been performed.
Accessory protein ID Locus tag (Gene) Type             Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Related structure protein Related effector
ACP00032 experimental EC042_RS24300 (tagA) structure affiliated protein Escherichia coli 042 chromosome (NC_017626) 4884070..4885437 (+) 446122356 TssA -
ACP00033 experimental EC042_RS24190 (tagL/ompA) structure affiliated protein Escherichia coli 042 chromosome (NC_017626) 4856944..4858674 (+) 446480629 TssL peptidoglycan
ACP00034 experimental EC042_1244 (mltE) structure affiliated protein Escherichia coli 042 chromosome (NC_017626) 1322321..1322932 (+) 481024453 TssM -
ACP00035 experimental EC042_RS24190 (sciZ) structure affiliated protein Escherichia coli 042 chromosome (NC_017626) 4856944..4858674 (+) 446480629 SciP
ACP00036 experimental EC042_RS21585 (yidC) chaperone Escherichia coli 042 chromosome (NC_017626) 4320019..4321665 (+) 446300403 TssL -
ACP00037 experimental EC042_RS00085 (dnaK) chaperone Escherichia coli 042 chromosome (NC_017626) 13600..15516 (+) 446438280 TssL -