SecReT6, T6SS database

Detailed information of immunity protein

Summary

Immunity protein ID	IMU00327
Cognate effector	EFF01498
Related T6SS (Type)	T6SS00905 (Type i1)
Strain	Escherichia coli strain TW-XM
Replicon	chromosome
Sequence	Protein sequence (97 a.a.); Nucleotide sequence (291 bp)
Description	A double knockout mutant lacking both xmtU and xmtV was efficiently reduced by fivefold when exposed to TW-XM or CΔxmtU, and protein binding between XmtU and XmtV was confirmed by a pull-down assay, indicating that XmtU/XmtV was a functional T6SS-related effector/immunity pair
Reference	[1] Ma J, Sun M, Pan Z, et al. Three Hcp homologs with divergent extended loop regions exhibit different functions in avian pathogenic Escherichia coli. Emerg Microbes Infect. 2018 Mar 29;7(1):49. doi: 10.1038/s41426-018-0042-0. PMID: 29593238

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	XmtV
Coordinates (Strand)	25611..25901 (+)
NCBI ID	611963724
GenBank	KF678350
Uniprot ID	Q6TKV7_ECOLX
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-96	MGIVFTNHNIDLLSVEFDEITKNCNYTFSVDGETAIFTARISIIRNIKGIKYSEELDKFIMSIMPLQPKVSKILGGVTWDCICGKEVGFPVRLIGK

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9958	-	-

Protein sequence: 97 a.a. .

                                    >IMU00327 KF678350:25611-25901 [Escherichia coli]
MGIVFTNHNIDLLSVEFDEITKNCNYTFSVDGETAIFTARISIIRNIKGIKYSEELDKFIMSIMPLQPKVSKILGGVTWD
CICGKEVGFPVRLIGK*

                                

Nucleotide sequence: 291 bp .

                                    >IMU00327 KF678350:25611-25901 [Escherichia coli]
ATGGGCATAGTGTTTACAAACCATAATATTGATTTGTTATCGGTTGAATTTGATGAGATTACAAAAAATTGTAATTACAC
TTTTAGTGTAGATGGAGAGACGGCTATCTTTACTGCTAGAATATCAATAATAAGAAATATAAAGGGAATTAAATATAGCG
AAGAACTTGATAAATTTATAATGTCAATAATGCCATTGCAGCCTAAAGTATCAAAAATTTTGGGAGGTGTTACTTGGGAT
TGTATTTGTGGTAAGGAAGTTGGATTTCCTGTGAGATTAATTGGTAAGTGA

                                