Detailed information of regulator
Summary
| Regulator ID | REG0271  |
| Regulator type | protein |
| Regulatory pathway (from literatures) | Global regulator |
| KEGG pathway (determined by BlastKOALA) | - |
| Related T6SS | T6SS00931 (Type i4b) |
| Strain | Escherichia coli strain 17-2 |
| Replicon | chromosome |
| Sequence | Protein sequence (149 a.a.); Nucleotide sequence (447 bp) |
| Description | Random mini-Tn10 transposon mutagenesis identified Fur as a regulator of EAEC sci1 expression. The activity of the sci1-lacZ reporter fusion in presence of the iron chelator 2,2′-dipyridyl increased upon exposure to dipyridyl and construction of the EAEC fur null strain confirmed the transposon mutagenesis data. |
| Reference |
External database links
| Locus tag (Gene) | H1D38_08255 (fur) |
| Coordinates (Strand) | 1744748..1745194 (-) |
| NCBI ID | MBA2233871.1 |
| GenBank | JACEFV010000001 |
| Uniprot ID | - |
| KEGG ID | - |
| KEGG pathway (determined by BlastKOALA) | - |
| PDB ID | - |
Target
| Target | Regulation (↓/↑) | Target sequence |
|---|---|---|
| sciH promotor | ↑ | TTCCATAGAATAAAGTCCTGATTATTTGCATTATATCGATCGATGTATCTGTTATATTGAGATTTTTCAGATCTTCGTCCTATAATGATCAAATTAAA The red sequences indicate Fur binding boxes and GATC Dam methylation sites are indicated in bold blue letters |
Pfam domain hit(s) of regulator
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| FUR | PF01475.21 | 1.4e-49 | 10..130 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-148 | MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLIDMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEFSDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9976 | - | - |
Protein sequence of regulator: 149 a.a. .
>REG0271 JACEFV010000001:c1745194-1744748 [Escherichia coli]
MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLIDMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVF
ELTQQHHHDHLICLDCGKVIEFSDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK*
MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLIDMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVF
ELTQQHHHDHLICLDCGKVIEFSDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK*
Nucleotide sequence of regulator: 447 bp .
>REG0271 JACEFV010000001:c1745194-1744748 [Escherichia coli]
ATGACTGATAACAATACCGCCCTAAAGAAAGCTGGCCTGAAAGTAACGCTTCCTCGTTTAAAAATCCTGGAAGTTCTTCA
GGAGCCGGACAACCATCACGTCAGTGCGGAAGATTTATACAAACGTCTGATCGATATGGGTGAAGAAATTGGTCTGGCTA
CGGTATATCGCGTACTGAACCAGTTTGACGACGCTGGTATCGTCACCCGCCACAATTTTGAAGGCGGTAAATCCGTATTT
GAACTGACACAGCAACATCACCACGATCACCTGATCTGCCTCGACTGCGGCAAGGTTATCGAATTTAGTGATGATTCCAT
CGAAGCGCGTCAGCGTGAAATTGCCGCAAAACATGGCATTCGCCTGACTAACCACAGTCTCTATCTTTACGGTCACTGTG
CCGAAGGCGATTGCCGCGAAGATGAACATGCGCACGAAGGCAAATAA
ATGACTGATAACAATACCGCCCTAAAGAAAGCTGGCCTGAAAGTAACGCTTCCTCGTTTAAAAATCCTGGAAGTTCTTCA
GGAGCCGGACAACCATCACGTCAGTGCGGAAGATTTATACAAACGTCTGATCGATATGGGTGAAGAAATTGGTCTGGCTA
CGGTATATCGCGTACTGAACCAGTTTGACGACGCTGGTATCGTCACCCGCCACAATTTTGAAGGCGGTAAATCCGTATTT
GAACTGACACAGCAACATCACCACGATCACCTGATCTGCCTCGACTGCGGCAAGGTTATCGAATTTAGTGATGATTCCAT
CGAAGCGCGTCAGCGTGAAATTGCCGCAAAACATGGCATTCGCCTGACTAACCACAGTCTCTATCTTTACGGTCACTGTG
CCGAAGGCGATTGCCGCGAAGATGAACATGCGCACGAAGGCAAATAA