SecReT6, T6SS database

Detailed information of effector

Summary

Effector ID	EFF01465
Effector type	Tle
Cognate immunity protein	IMU00313
Related T6SS (Type)	T6SS00931 (Type i4b)
Strain	Escherichia coli 042
Replicon	chromosome
Sequence	Protein sequence (561 a.a.); Nucleotide sequence (1683 bp)
Description	Tle1^EAEC
Reference	[1] Flaugnatti N, Le TT, Canaan S, et al. A phospholipase A1 antibacterial Type VI secretion effector interacts directly with the C-terminal domain of the VgrG spike protein for delivery. Mol Microbiol. 2016 Mar;99(6):1099-118. doi: 10.1111/mmi.13292. Epub 2016 Feb 10. PMID: 26714038

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	EC042_RS24220
Coordinates (Strand)	4865605..4867287 (+)
NCBI ID	284924255
RefSeq	NC_017626
Uniprot ID	D3GUW5_ECO44
KEGG ID	elo:EC042_4534
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
DUF2235	PF09994.11	6.4e-10	233..320
DUF2235	PF09994.11	4.7e-06	114..210

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-560	MTKYQGYDVTDATHKTSIHNDWKVVVAKKKPARGVTLTIGIFFDGTGNNRENTASRLMKFNECSAARQGVNQKDAQSCEDFLKEINKNSISNGSYRGYYSNIHWLNILYHPDQVLKKDQTSAQIKTYISGIGTAAGEADSVIGMGLGTSILDIFEGVVTKTDEAMERITQALSEFMGFNLSPDFCIAKIQFDVFGFSRGAAAARHFANRVMEQDPAIARAIAKGLRGDFYDGKPSGEVRFLGLFDTVAAIGGISNFFDINGRSNPGVKLELRPSVAKKVFQITAMNEYRYNFSLNSIKGMWPELALPGAHSDIGGGYNPVGSPLQENESLFLSCPEFEIVSDDTREMDTRVYRKAEQVRKMLMTLPALKHILPHGKLTTKIRSIGVNNSNQRRAGVIQKQVGAAVFFERMAVPNDWANVCLRVMLDAAQEAGVLFEPIRQTNTELQLPSELIFLADKAIAQGKAVRLGQEPQAFTEEELYIIGKYTHCSANWNIESDGNLWVDPTTGEIFIHRFGPKGNKAFVFPNKPNDRWIRSVWYMDDQQRLNDNAVKNTKVMMSGV

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9863	-	-

Protein sequence: 561 a.a. .

                                    >EFF01465 NC_017626:4865605-4867287 Type VI Lipase Effector (Tle) [Escherichia coli 042]
MTKYQGYDVTDATHKTSIHNDWKVVVAKKKPARGVTLTIGIFFDGTGNNRENTASRLMKFNECSAARQGVNQKDAQSCED
FLKEINKNSISNGSYRGYYSNIHWLNILYHPDQVLKKDQTSAQIKTYISGIGTAAGEADSVIGMGLGTSILDIFEGVVTK
TDEAMERITQALSEFMGFNLSPDFCIAKIQFDVFGFSRGAAAARHFANRVMEQDPAIARAIAKGLRGDFYDGKPSGEVRF
LGLFDTVAAIGGISNFFDINGRSNPGVKLELRPSVAKKVFQITAMNEYRYNFSLNSIKGMWPELALPGAHSDIGGGYNPV
GSPLQENESLFLSCPEFEIVSDDTREMDTRVYRKAEQVRKMLMTLPALKHILPHGKLTTKIRSIGVNNSNQRRAGVIQKQ
VGAAVFFERMAVPNDWANVCLRVMLDAAQEAGVLFEPIRQTNTELQLPSELIFLADKAIAQGKAVRLGQEPQAFTEEELY
IIGKYTHCSANWNIESDGNLWVDPTTGEIFIHRFGPKGNKAFVFPNKPNDRWIRSVWYMDDQQRLNDNAVKNTKVMMSGV
*

                                

Nucleotide sequence: 1683 bp .

                                    >EFF01465 NC_017626:4865605-4867287 Type VI Lipase Effector (Tle) [Escherichia coli 042]
ATGACAAAATATCAGGGATATGACGTAACAGATGCCACCCATAAAACCAGTATTCATAATGACTGGAAAGTTGTTGTTGC
AAAGAAAAAACCGGCGCGGGGTGTGACTTTAACTATTGGCATATTTTTCGATGGTACCGGAAACAATCGGGAGAATACAG
CATCTCGCCTGATGAAATTTAATGAGTGTAGTGCAGCCAGACAGGGGGTAAATCAAAAAGATGCACAAAGCTGTGAGGAC
TTTCTTAAAGAAATTAACAAGAACAGTATCTCGAATGGCAGTTATCGTGGATATTATTCCAATATTCACTGGCTTAATAT
ACTCTATCATCCAGATCAGGTACTTAAAAAAGACCAGACGTCAGCTCAAATTAAGACTTACATTTCCGGGATAGGGACTG
CGGCTGGTGAAGCAGATTCAGTCATAGGCATGGGATTAGGCACCAGTATTCTGGATATATTTGAAGGTGTGGTGACGAAA
ACCGACGAGGCAATGGAACGAATCACCCAGGCGTTATCAGAATTTATGGGGTTCAATTTAAGTCCTGATTTTTGTATCGC
AAAAATCCAGTTTGATGTTTTTGGTTTTAGCCGGGGAGCAGCGGCGGCAAGGCACTTTGCCAATCGGGTTATGGAACAGG
ATCCTGCTATAGCCAGAGCTATTGCTAAAGGGCTCAGAGGCGATTTTTATGATGGTAAACCTTCTGGTGAAGTACGTTTT
CTGGGGCTTTTTGATACTGTCGCAGCTATTGGTGGAATCAGTAATTTCTTCGATATTAATGGACGTAGCAACCCGGGAGT
AAAGCTGGAGTTGCGTCCTTCAGTCGCGAAGAAAGTTTTCCAGATTACTGCAATGAATGAATACCGATATAATTTCAGCC
TGAACAGTATTAAAGGTATGTGGCCCGAACTGGCTTTACCGGGGGCGCATTCAGATATTGGAGGTGGTTACAATCCGGTA
GGTTCACCTCTGCAGGAAAATGAAAGTCTGTTTCTGAGTTGCCCGGAATTTGAAATTGTCAGTGACGATACCAGAGAAAT
GGATACGCGAGTATACCGTAAAGCAGAGCAAGTCAGAAAAATGTTGATGACTCTGCCAGCCTTAAAACATATTTTGCCTC
ATGGAAAACTAACCACCAAAATTCGTTCCATAGGTGTGAACAACAGCAATCAACGTCGGGCTGGTGTGATACAAAAGCAG
GTGGGAGCCGCAGTATTTTTTGAGCGTATGGCAGTACCTAATGACTGGGCTAATGTTTGCCTGCGTGTGATGCTGGATGC
TGCTCAGGAAGCAGGGGTATTGTTTGAACCAATTCGACAAACAAATACTGAGTTACAACTTCCATCTGAACTAATATTTC
TTGCTGACAAAGCCATTGCTCAGGGAAAAGCGGTACGTTTGGGGCAAGAGCCTCAGGCTTTTACTGAGGAAGAGTTGTAT
ATTATCGGAAAATACACCCATTGTTCCGCTAACTGGAATATTGAGTCTGATGGTAATTTATGGGTGGATCCTACAACGGG
AGAGATTTTTATCCATCGCTTTGGTCCTAAAGGTAATAAGGCGTTCGTTTTTCCGAATAAACCAAATGATCGCTGGATTC
GTTCTGTATGGTACATGGATGATCAGCAGCGATTGAATGATAACGCAGTAAAAAATACTAAAGTTATGATGTCTGGTGTT
TAA

                                