SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP005148
Component protein type	TssL
T6SS ID (Type)	T6SS00464 (Type i4b)
Strain	Escherichia coli 042
Replicon	chromosome
Sequence	Protein sequence (197 a.a.); Nucleotide sequence (591 bp)
Reference	[1] Dudley EG, Thomson NR, Parkhill J, et al. Proteomic and microarray characterization of the AggR regulon identifies a pheU pathogenicity island in enteroaggregative Escherichia coli. Mol Microbiol. 2006 Sep;61(5):1267-82. PMID: 16925558 [2] Morin N, Santiago AE, Ernst RK, et al. Characterization of the AggR regulon in enteroaggregative Escherichia coli. Infect Immun. 2013 Jan;81(1):122-32. doi: 10.1128/IAI.00676-12. Epub 2012 Oct 22. PMID: 23090962

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	EC042_RS24425
Coordinate (Strand)	4904890..4905480 (+)
NCBI ID	WP_001028111.1
RefSeq	NC_017626
Uniprot ID	D3GV02_ECO44, A0A3J8YF41_ECOLX
KEGG ID	elo:EC042_4575
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
DotU	PF09850.11	3.6e-31	3..194

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-172	VNIVECYMPVFKLISSVRVFPEEYSDYDSTRKNIINTVEEAVRNSEKMSLSDSELDAAFYAVIVMLDEAILCSELPCRKEWRDNLLQIKYFGHSTGGVEFFYKLDKVIESGSQAGWVFLLCLLLGFRGKYSVGSNDEINNYISRLRRQCELNVAIDEGNNISLREKKYKSSR
Transmembrane helix	173-195	LGFYLLLVGIVAVYFVVLSMIYM
Outside	196-196	R

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9766	-	-

Protein sequence: 197 a.a.

						>T6CP005148 NC_017626:4904890-4905480 [Escherichia coli 042] [TssL]
VNIVECYMPVFKLISSVRVFPEEYSDYDSTRKNIINTVEEAVRNSEKMSLSDSELDAAFYAVIVMLDEAILCSELPCRKE
WRDNLLQIKYFGHSTGGVEFFYKLDKVIESGSQAGWVFLLCLLLGFRGKYSVGSNDEINNYISRLRRQCELNVAIDEGNN
ISLREKKYKSSRLGFYLLLVGIVAVYFVVLSMIYMR*					

Nucleotide sequence: 591 bp

						>T6CP005148 NC_017626:4904890-4905480 [Escherichia coli 042] [TssL]
GTGAATATTGTTGAATGCTATATGCCAGTATTTAAACTCATATCATCTGTCAGAGTTTTCCCTGAAGAATATAGTGATTA
CGACAGTACTAGAAAGAATATAATTAACACTGTTGAGGAGGCTGTTCGCAATAGTGAAAAAATGTCGTTGAGTGACAGCG
AACTTGATGCGGCATTTTATGCTGTAATCGTCATGCTTGATGAGGCAATATTGTGTTCAGAGCTTCCCTGTAGAAAAGAA
TGGCGTGATAATCTTTTGCAGATAAAATATTTCGGACACTCCACTGGGGGGGTCGAGTTCTTTTATAAACTTGATAAAGT
CATCGAATCGGGAAGTCAGGCTGGATGGGTCTTTCTCCTGTGCCTGTTACTGGGTTTCAGAGGTAAATATTCCGTGGGTA
GTAATGATGAAATCAATAATTATATATCGAGGCTGAGGCGACAATGTGAATTGAATGTTGCAATCGACGAAGGAAACAAT
ATTTCACTCAGGGAAAAAAAGTATAAATCATCGAGGCTTGGTTTCTATTTATTACTTGTAGGTATTGTTGCTGTCTATTT
TGTGGTTTTATCCATGATTTATATGAGATGA					