Detailed information of component protein


Summary

Component ID T6CP005136
Component protein type TssM
T6SS ID (Type) T6SS00463 experimental (Type i2)
Strain Escherichia coli 042
Replicon chromosome
Sequence Protein sequence (1130 a.a.); Nucleotide sequence (3390 bp)
Reference
[1] Durand E, Zoued A, Spinelli S, et al. Structural characterization and oligomerization of the TssL protein, a component shared by bacterial type VI and type IVb secretion systems. J Biol Chem. 2012 Apr 20;287(17):14157-68. doi: 10.1074/jbc.M111.338731. Epub 2012 Feb 27. PMID: 22371492
[2] Felisberto-Rodrigues C, Durand E, Aschtgen MS, et al. Towards a structural comprehension of bacterial type VI secretion systems: characterization of the TssJ-TssM complex of an Escherichia coli pathovar. PLoS Pathog. 2011 Nov;7(11):e1002386. doi: 10.1371/journal.ppat.1002386. Epub 2011 Nov 10. PMID: 22102820
[3] Dudley EG, Thomson NR, Parkhill J, et al. Proteomic and microarray characterization of the AggR regulon identifies a pheU pathogenicity island in enteroaggregative Escherichia coli. Mol Microbiol. 2006 Sep;61(5):1267-82. PMID: 16925558
[4] Morin N, Santiago AE, Ernst RK, et al. Characterization of the AggR regulon in enteroaggregative Escherichia coli. Infect Immun. 2013 Jan;81(1):122-32. doi: 10.1128/IAI.00676-12. Epub 2012 Oct 22. PMID: 23090962
[5] Zoued A, Durand E, Bebeacua C, et al. TssK is a trimeric cytoplasmic protein interacting with components of both phage-like and membrane anchoring complexes of the type VI secretion system. J Biol Chem. 2013 Sep 20;288(38):27031-27041. doi: 10.1074/jbc.M113.499772. Epub 2013 Aug 6. PMID: 23921384
experimental Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene) EC042_RS24245
Coordinate (Strand) 4870248..4873637 (+)
NCBI ID WP_001037088.1
RefSeq NC_017626
Uniprot ID A0A3W4JPH2_ECOLX, B7LFU0_ECO55, D3GUX0_ECO44
KEGG ID elo:EC042_4539, eck:EC55989_3325
PDB ID 6IXH

Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related PF06761.14 1.5e-61 409..725
IcmF_C PF06744.14 4.6e-19 979..1085

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-14 MNKLACLSGRFGRP
Transmembrane helix 15-37 GIVFIGVAALWWLITRYGAYLGA
Inside 38-43 ETRRDQ
Transmembrane helix 44-63 ILLLILLSLGVLFVCYLPVM
Outside 64-1129 KKYVQELTYRRRARKEQRLPDDEERLAQTPPRYVTVQDIRHTLRRQYGRFWGRKIRILLITGTASEVELLTPGLTEQFWQEEQGTLLLWGGDPSQPENADWLAALRRLRYRPADGIVWVTSGLSETLSAPLTEDALDRVSRAVSSCCERLGWRLPLYVWSLQESPDERGRITQPVGCLLPAECSSDKLKAQLQAMLPGLVAQGIQQICCAPRYYFLLSLAERFRRNIDAVVEPLSVLLRPYRQLLLAGIVFSPATVGGERSVRHRWRMDNRWEALPETVQQLPVRLQPSRTGHNWRRSLAVMAAILMMAQGTGMVVSFLANRSLVAEVQEQIRPAQNQQLSPAERLQALLNLQKSLARLQYREEHGAPWYLRAGMNQNADLLAVVMPLYAQNAHLLLRDAAAAHLEQQLRTFIRLPPDSPQRGKMAKAAYDQLRLYLMLAQPQHMEPAWFSRTLMREWPQRDGVSAVFWQANGPTLLAYYASGIITHPQWKLTADEELVSQSRTLLLRHLGTQNSDAMLYQKMLARVAHQFADMRLTDMTGDTDVSRLFFTDEVVPGMFTRQAWEEAVLPSIDTVINERREEMDWVLTDGRQKAPSPVSPEALRQRLTTRYFADFGNAWLNFLNSLHLRKAQTLSDVTEQLTLMADVRQSPLVALMNTLAVQGCTGQPREAVTDSLVKSARNLLSQEKQPVAVPESRLHGPLATTFGPVLALMDNQNNSADMLNLQTYLTRVTQVRLRLQQIAGSSDPQAMMQLLAQTVLQGKSVDLTDTRDYGSLTAAGLGQEWYGFGQTVFVRPMEQAWQQVLTPAAESLNARWRTAVVDGWNNAFSGRYPFKNVSSDASLPLLAKYLNTDTGRIARFLQNNLSGVLHREGSRWVPDTINTRGLTFNPAFLKAINTLSEIADVAFTTGNAGLHFELRPGTAAGVMQTTLITDNQKLIYVNQMPVWKRFTWPADTEAPGASLSWVSTQAGTRQYADLPGSWGLIRLLEMARRKAAPGVASGWSLSWQAQDGRMLNYTLRTEAGEGPLVLLKLRNFVLPETVFELSGTSAFTGNDEDAGDTVEETD



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9959 - -



  Protein sequence: 1130 a.a.    

>T6CP005136 NC_017626:4870248-4873637 [Escherichia coli 042] [TssM]
MNKLACLSGRFGRPGIVFIGVAALWWLITRYGAYLGAETRRDQILLLILLSLGVLFVCYLPVMKKYVQELTYRRRARKEQ
RLPDDEERLAQTPPRYVTVQDIRHTLRRQYGRFWGRKIRILLITGTASEVELLTPGLTEQFWQEEQGTLLLWGGDPSQPE
NADWLAALRRLRYRPADGIVWVTSGLSETLSAPLTEDALDRVSRAVSSCCERLGWRLPLYVWSLQESPDERGRITQPVGC
LLPAECSSDKLKAQLQAMLPGLVAQGIQQICCAPRYYFLLSLAERFRRNIDAVVEPLSVLLRPYRQLLLAGIVFSPATVG
GERSVRHRWRMDNRWEALPETVQQLPVRLQPSRTGHNWRRSLAVMAAILMMAQGTGMVVSFLANRSLVAEVQEQIRPAQN
QQLSPAERLQALLNLQKSLARLQYREEHGAPWYLRAGMNQNADLLAVVMPLYAQNAHLLLRDAAAAHLEQQLRTFIRLPP
DSPQRGKMAKAAYDQLRLYLMLAQPQHMEPAWFSRTLMREWPQRDGVSAVFWQANGPTLLAYYASGIITHPQWKLTADEE
LVSQSRTLLLRHLGTQNSDAMLYQKMLARVAHQFADMRLTDMTGDTDVSRLFFTDEVVPGMFTRQAWEEAVLPSIDTVIN
ERREEMDWVLTDGRQKAPSPVSPEALRQRLTTRYFADFGNAWLNFLNSLHLRKAQTLSDVTEQLTLMADVRQSPLVALMN
TLAVQGCTGQPREAVTDSLVKSARNLLSQEKQPVAVPESRLHGPLATTFGPVLALMDNQNNSADMLNLQTYLTRVTQVRL
RLQQIAGSSDPQAMMQLLAQTVLQGKSVDLTDTRDYGSLTAAGLGQEWYGFGQTVFVRPMEQAWQQVLTPAAESLNARWR
TAVVDGWNNAFSGRYPFKNVSSDASLPLLAKYLNTDTGRIARFLQNNLSGVLHREGSRWVPDTINTRGLTFNPAFLKAIN
TLSEIADVAFTTGNAGLHFELRPGTAAGVMQTTLITDNQKLIYVNQMPVWKRFTWPADTEAPGASLSWVSTQAGTRQYAD
LPGSWGLIRLLEMARRKAAPGVASGWSLSWQAQDGRMLNYTLRTEAGEGPLVLLKLRNFVLPETVFELSGTSAFTGNDED
AGDTVEETD*

  Nucleotide sequence: 3390 bp    

>T6CP005136 NC_017626:4870248-4873637 [Escherichia coli 042] [TssM]
ATGAATAAACTGGCCTGTCTGTCTGGTCGCTTTGGGCGACCGGGGATTGTATTTATTGGTGTGGCTGCGCTCTGGTGGCT
GATAACCCGATATGGAGCATATCTGGGGGCAGAAACACGCCGGGATCAAATACTACTGCTGATATTGCTGAGTCTGGGGG
TGCTCTTTGTCTGTTACCTGCCTGTGATGAAAAAATATGTTCAGGAACTGACATATCGACGCAGGGCCCGTAAGGAGCAA
AGGTTACCTGACGATGAAGAGCGATTGGCTCAGACGCCACCGCGCTATGTTACTGTTCAGGATATTCGTCATACTCTGCG
GCGCCAGTATGGCAGATTCTGGGGACGTAAGATACGCATTCTGTTGATAACAGGTACCGCCTCTGAGGTTGAGTTGTTGA
CCCCGGGCCTGACAGAACAGTTCTGGCAGGAAGAGCAGGGGACATTGCTTCTCTGGGGAGGAGACCCCTCGCAGCCGGAA
AACGCAGACTGGCTGGCTGCGTTACGCCGTCTGCGTTACCGGCCTGCTGATGGCATTGTCTGGGTAACTTCCGGACTGAG
TGAAACATTATCCGCACCACTGACAGAAGATGCCCTGGACAGGGTTTCCCGTGCGGTCAGTTCATGTTGTGAGCGCCTTG
GCTGGCGTCTGCCGCTGTATGTCTGGTCACTGCAGGAGAGTCCGGATGAGCGAGGGCGTATTACTCAGCCCGTGGGATGC
CTTCTGCCAGCAGAATGTAGTTCTGACAAACTGAAAGCACAACTACAGGCCATGCTCCCCGGGCTTGTTGCTCAGGGGAT
ACAGCAGATATGCTGTGCCCCTCGGTATTATTTTTTGTTGTCGCTGGCAGAGCGATTCAGACGAAATATTGATGCTGTGG
TGGAGCCATTATCCGTATTACTCCGTCCATACCGTCAGTTGTTGCTGGCGGGTATTGTTTTCAGTCCGGCAACTGTCGGC
GGTGAACGAAGTGTCAGACATCGCTGGCGAATGGATAACCGCTGGGAGGCGTTGCCGGAGACAGTGCAGCAATTACCTGT
CCGGCTACAGCCTTCCCGCACAGGTCATAACTGGCGCAGAAGTCTGGCCGTTATGGCGGCCATACTGATGATGGCTCAGG
GAACAGGAATGGTGGTTTCCTTTCTGGCAAACCGGAGTCTGGTTGCTGAAGTACAGGAACAGATTCGTCCGGCTCAGAAC
CAGCAACTGTCTCCTGCAGAACGTCTGCAGGCGTTGCTGAATCTGCAGAAATCCCTGGCCCGCCTTCAGTACCGGGAGGA
GCACGGGGCGCCATGGTACCTGCGGGCCGGTATGAATCAGAATGCAGACCTGCTCGCGGTCGTAATGCCCCTTTATGCAC
AAAATGCACATCTTTTACTGCGTGATGCCGCTGCTGCTCATCTTGAGCAGCAGCTCAGGACATTTATTCGTCTTCCTCCG
GACAGCCCGCAGCGGGGGAAAATGGCAAAAGCCGCATATGACCAGCTCAGACTGTATCTGATGCTGGCGCAACCGCAACA
TATGGAGCCCGCCTGGTTCAGTCGTACGCTCATGCGGGAATGGCCACAGCGTGACGGTGTGAGTGCCGTTTTCTGGCAGG
CTAATGGCCCGACGTTGCTGGCGTATTATGCCTCCGGGATTATTACTCATCCTCAGTGGAAGCTGACGGCTGATGAAGAG
CTGGTCAGCCAGAGCAGAACTCTGCTGCTTCGCCATCTGGGAACACAGAACAGCGATGCCATGCTGTATCAGAAAATGCT
GGCACGGGTGGCACATCAGTTTGCGGATATGCGTCTGACGGATATGACCGGTGATACTGATGTCTCCCGCCTCTTTTTTA
CGGATGAAGTGGTTCCCGGTATGTTTACCCGCCAGGCCTGGGAAGAGGCCGTATTGCCGTCGATAGACACCGTGATTAAT
GAGCGTCGTGAAGAAATGGACTGGGTACTGACGGATGGCAGGCAGAAGGCACCGTCACCGGTATCGCCGGAAGCACTCAG
ACAGCGCCTGACAACGCGCTATTTTGCTGATTTTGGTAATGCCTGGCTGAATTTTCTGAACAGTCTGCACTTGCGTAAAG
CTCAGACGCTGTCTGATGTGACAGAGCAACTGACGCTTATGGCTGATGTGCGCCAGTCCCCCCTGGTTGCTCTGATGAAT
ACGCTGGCGGTTCAGGGGTGTACTGGCCAGCCCCGGGAAGCGGTTACTGATTCACTGGTTAAATCTGCCAGAAACCTGCT
TTCACAGGAAAAACAGCCAGTGGCAGTACCGGAAAGCCGGCTTCACGGTCCTCTGGCGACGACTTTCGGGCCGGTGCTTG
CACTGATGGATAATCAGAACAACAGTGCGGATATGCTGAATCTGCAGACATATCTGACCCGGGTGACACAGGTTCGTCTG
CGTCTGCAACAGATTGCCGGCTCTTCTGACCCGCAGGCCATGATGCAGTTGCTGGCACAGACAGTATTGCAGGGGAAATC
GGTTGATTTAACCGATACCCGGGATTACGGCAGCCTGACCGCTGCCGGACTCGGACAGGAGTGGTATGGCTTCGGACAGA
CGGTCTTTGTTCGCCCGATGGAGCAGGCGTGGCAGCAGGTTCTTACGCCCGCGGCAGAAAGTCTGAATGCCCGGTGGCGT
ACTGCGGTGGTGGATGGCTGGAATAATGCATTTTCCGGGCGTTATCCGTTTAAAAATGTCAGCAGCGATGCTTCGCTTCC
CCTGCTGGCAAAATACCTGAATACGGACACGGGACGTATTGCCCGTTTCCTGCAGAATAACCTGAGTGGTGTGTTGCACA
GGGAAGGCAGTCGCTGGGTACCTGATACCATTAATACCCGGGGACTGACATTCAACCCTGCGTTTCTGAAAGCCATCAAT
ACCCTGAGTGAAATAGCGGATGTGGCGTTCACCACAGGTAACGCGGGGCTGCATTTTGAGCTGCGCCCGGGAACTGCTGC
CGGTGTGATGCAGACGACGCTGATAACCGATAATCAGAAACTGATTTATGTTAATCAGATGCCGGTATGGAAGCGATTTA
CCTGGCCGGCTGATACGGAAGCACCTGGCGCCAGTTTAAGCTGGGTAAGCACTCAGGCCGGTACCCGTCAGTATGCAGAC
CTGCCGGGAAGCTGGGGGCTGATACGTCTGCTTGAGATGGCCCGACGGAAAGCCGCACCGGGCGTTGCCAGTGGCTGGAG
CCTGAGCTGGCAGGCGCAGGACGGTCGTATGCTGAATTACACACTGCGAACTGAAGCGGGGGAAGGGCCGCTTGTATTGC
TGAAACTCCGCAATTTTGTTCTGCCGGAAACGGTTTTCGAACTCTCCGGCACGTCAGCGTTTACAGGCAATGACGAAGAT
GCCGGGGATACCGTGGAGGAGACTGACTGA