Detailed information of Recipient susceptibility factor


Summary

ID RSF000061 experimental
Strain Escherichia coli strain MOD1-EC300
Replicon chromosome
Sequence Protein sequence (379 a.a.); Nucleotide sequence (1137 bp)
Response to T6SS attack protective
Known or putative function capsule protection
Description Consistent with the encapsulation of Ec300, the WT (CPS+) was resistant to T6SS attacks, while the survival of the non- encapsulated mutant (CPS-) as well as the K-12 and MG1655 strains was significantly reduced by V. cholerae.
Reference
experimental Experimental investigation has been performed.
External database links

Locus tag (Gene) BSJ02_RS22110 (wza)
Coordinates (Strand) 16803..17939 (-)
NCBI ID WP_096838211.1
RefSeq NZ_NJMM01000062
Uniprot ID UPI0007BADE02
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Poly_export PF02563.18 3.2e-28 82..165
Wza_C PF18412.3 3e-17 345..374
SLBB PF10531.11 1.3e-08 173..222
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-4 MKKK
Transmembrane helix 5-27 IVRFSALALAIGFLSGCTIIPGQ
Outside 28-378 GLNSLRKNVVELPDSDYDLDKLVNVYPMTPGLIDQLRPETVLARPNPQLDNLLRSYEYRIGVGDVLMVTVWDHPELTTPAGQYRSASDTGNWVNSDGTIFYPYIGKVQVAGKTLSQVRQEIASRLTTYIESPQVDVSVAAFRSQKAYVTGEVAKSGQQPITNIPLTVMDAINNAGGLAPDADWRNVVLTHNGKDTKISLYALMQKGDLTQNHLLYPGDILFVPRNDDLKVFVMGEVGKQSTLKMDRSGMTVAEALGNAEGMSQAFSDATGVFVIRQLKGDKQGKIANIYQLNAQDASAMVLGTEFQLQPYDIVYVTTAPLVRWNRVISQLVPTITGVHDMTETTKFIKDWP


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
LIPO(Sec/SPII) 0.9982 pos: 20-21. LSG-CT. MKKKIVRFSALALAIGFLSG


  Protein sequence: 379 a.a.    .

>RSF000061 NZ_NJMM01000062:c17939-16803 [Escherichia coli]
MKKKIVRFSALALAIGFLSGCTIIPGQGLNSLRKNVVELPDSDYDLDKLVNVYPMTPGLIDQLRPETVLARPNPQLDNLL
RSYEYRIGVGDVLMVTVWDHPELTTPAGQYRSASDTGNWVNSDGTIFYPYIGKVQVAGKTLSQVRQEIASRLTTYIESPQ
VDVSVAAFRSQKAYVTGEVAKSGQQPITNIPLTVMDAINNAGGLAPDADWRNVVLTHNGKDTKISLYALMQKGDLTQNHL
LYPGDILFVPRNDDLKVFVMGEVGKQSTLKMDRSGMTVAEALGNAEGMSQAFSDATGVFVIRQLKGDKQGKIANIYQLNA
QDASAMVLGTEFQLQPYDIVYVTTAPLVRWNRVISQLVPTITGVHDMTETTKFIKDWP*

  Nucleotide sequence: 1137 bp    .

>RSF000061 NZ_NJMM01000062:c17939-16803 [Escherichia coli]
ATGAAGAAAAAAATTGTTAGATTTTCGGCATTAGCGTTGGCAATTGGGTTTTTATCAGGTTGTACCATTATCCCTGGTCA
GGGATTAAACAGTCTGCGTAAGAACGTGGTTGAACTCCCAGATAGTGATTACGATTTGGATAAGTTGGTCAATGTATATC
CGATGACACCAGGATTGATTGATCAACTTCGCCCAGAAACCGTTCTGGCTCGTCCCAATCCGCAACTGGATAACTTACTC
CGTAGTTATGAATATCGCATTGGGGTAGGTGACGTACTGATGGTTACCGTATGGGACCACCCTGAACTAACTACTCCTGC
AGGCCAATACCGCAGCGCCAGCGACACCGGTAACTGGGTCAACTCCGATGGCACCATTTTCTATCCTTATATCGGTAAGG
TACAGGTGGCGGGAAAAACCTTGAGCCAGGTACGCCAGGAAATAGCGAGTCGTTTGACTACCTATATTGAAAGTCCACAG
GTTGATGTTAGCGTTGCTGCATTCCGTTCGCAAAAAGCATATGTTACTGGTGAAGTTGCAAAATCAGGACAACAGCCCAT
CACGAATATCCCATTAACAGTCATGGATGCGATTAATAATGCTGGTGGATTGGCACCAGACGCAGACTGGCGTAACGTGG
TGCTGACTCATAATGGTAAAGATACAAAAATCTCACTTTACGCATTAATGCAGAAAGGGGACTTGACGCAGAATCATCTG
CTTTATCCCGGCGATATACTTTTTGTACCACGTAATGATGATTTGAAAGTTTTTGTCATGGGTGAGGTGGGCAAACAAAG
TACCTTAAAAATGGACCGCAGCGGTATGACCGTTGCTGAAGCATTAGGAAATGCTGAAGGAATGTCGCAGGCGTTCAGTG
ACGCAACAGGTGTGTTTGTAATTCGCCAGTTGAAAGGTGATAAACAAGGTAAAATAGCTAACATTTATCAACTTAATGCG
CAAGATGCTTCGGCAATGGTACTGGGAACAGAATTTCAGTTACAACCATACGATATCGTTTATGTCACAACTGCTCCTTT
AGTACGTTGGAATCGTGTGATTTCTCAGCTTGTGCCGACTATTACCGGTGTTCATGACATGACGGAAACTACGAAATTTA
TTAAGGATTGGCCATAG