SecReT6, T6SS database

Detailed information of Recipient susceptibility factor

Summary

ID	RSF000018
Strain	Escherichia coli BW25113 strain K-12
Replicon	chromosome
Sequence	Protein sequence (431 a.a.); Nucleotide sequence (1293 bp)
Response to T6SS attack	protective
Known or putative function	Tol-Pal system protein
Description	When co-incubated with T6SS-active 4effc compared with the ΔvasK mutant, whereas the tolB mutant showed considerable cell lysis but a nonsignificant difference between 4effc and ΔvasK. Competition assays show that survival of tolB and rseA was significantly reduced by 4effc.
Reference	[1] Kamal F, Liang X, Manera K, et al. Differential Cellular Response to Translocated Toxic Effectors and Physical Penetration by the Type VI Secretion System. Cell Rep. 2020 Jun 16;31(11):107766. doi: 10.1016/j.celrep.2020.107766. PMID: 32553162 [2] Hersch SJ, Manera K, Dong TG. Defending against the Type Six Secretion System: beyond Immunity Genes. Cell Rep. 2020 Oct 13;33(2):108259. doi: 10.1016/j.celrep.2020.108259. PMID: 33053336

Experimental investigation has been performed.

External database links

Locus tag (Gene)	BW25113_RS03840 (tolB)
Coordinates (Strand)	773196..774488 (+)
NCBI ID	481023805
RefSeq	NZ_CP009273
Uniprot ID	A0A1Z3UTA2_ECOLX, M9GA35_ECOLX, A0A0E0Y2D6_ECO1C, K4X2P0_ECOLX, S1PL24_ECOLX, L3BQQ2_ECOLX, S1ETH5_ECOLX, L3QCT1_ECOLX, S1I4G8_ECOLX, L4JFQ5_ECOLX, A0A0E2LMS3_ECOU3, A0A080JCX5_ECOLX, A0A029I9E5_ECOLX, A0A0A0F9G1_ECOLX, TOLB_ECOL6, D3GY88_ECO44, TOLB_ECODH, E3PHC9_ECOH1, TOLB_ECO24, A0A0E1T5V1_ECOLX, TOLB_ECOL5, TOLB_ECOUT, TOLB_ECO5E, A0A140NEI9_ECOBD, J7QYX5_ECOLX, A0A0H3PPH9_ECO5C, TOLB_ECOLC, D6I6W7_ECOLX, D6J861_ECOLX, E0J6E7_ECOLW, A0A168UEE2_KLEOX, TOLB_ECO27, TOLB_ECO45, TOLB_ECO55, TOLB_ECOLU, TOLB_ECO7I, TOLB_ECO81, TOLB_ECOBW, A0A237F8P9_SHIBO, A0A0H9UZS2_SHISO, A0A1X3JK31_ECOLX, A0A1X3L3Z9_ECOLX, F4VBH1_ECOLX, F4SL32_ECOLX, A0A1X3I8M7_ECOLX, A0A0K9TEN3_ECOLX, F4SWU9_ECOLX, F4TBV4_ECOLX, A0A1X3LGP8_ECOLX, A0A1X3J4D1_ECOLX, E9XNR8_ECOLX, A0A0H3EGL3_ECO8N, A0A0E1LZ85_ECOLX, I4SM07_ECOLX, TOLB_ECOLI, TOLB_ECO57, I2UMH1_ECOLX, I2WSF6_ECOLX
KEGG ID	ece:Z0908, ecp:ECP_0751, ecg:E2348C_0624, ecj:JW5100, eln:NRG857_03300, elw:ECW_m0795, esl:O3K_17935, eck:EC55989_0725, ecf:ECH74115_0843, ecs:ECs0775, elh:ETEC_0751, eco:b0740, ebd:ECBD_2920, ecq:ECED1_0707, eci:UTI89_C0736, ecl:EcolC_2915, ebw:BWG_0599, ecc:c0819, elo:EC042_0766, ect:ECIAI39_0715, ecw:EcE24377A_0769, eum:ECUMN_0828, ecz:ECS88_0763, ecd:ECDH10B_0807
PDB ID	7NSU, 2W8B, 1CRZ, 7NST, 1C5K, 2IVZ, 3IAX, 2HQS

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
TolB_N	PF04052.15	7.1e-30	24..121
PD40	PF07676.14	5.7e-10	281..315
PD40	PF07676.14	4.8e-09	237..272

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-430	MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQWAGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVGQVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIKGAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVTFESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLASGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQNQDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYSSSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
SP(Sec/SPI)	0.9821	pos: 21-22. LHA-EV.	MKQALRVAFGFLILWASVLHA

Protein sequence: 431 a.a. .

                                    >RSF000018 NZ_CP009273:773196-774488 [Escherichia coli BW25113]
MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQWAGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQP
GSAQEVQPAAWSALGIDAVVVGQVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVTFESGRSALVIQTLANGAVRQVA
SFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLASGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNIN
GGAPQRITWEGSQNQDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYSSSQGMGSV
LNLVSTDGRFKARLPATDGQVKFPAWSPYL*

                                

Nucleotide sequence: 1293 bp .

                                    >RSF000018 NZ_CP009273:773196-774488 [Escherichia coli BW25113]
ATGAAGCAGGCATTACGAGTAGCATTTGGTTTTCTCATACTGTGGGCATCAGTTCTGCATGCTGAAGTCCGCATTGTGAT
CGACAGCGGTGTAGATTCCGGTCGTCCTATTGGTGTTGTTCCTTTCCAGTGGGCGGGGCCTGGTGCGGCACCTGAAGATA
TTGGCGGCATCGTTGCTGCTGACTTGCGTAACAGCGGTAAATTTAATCCGTTAGATCGCGCTCGTCTGCCACAGCAGCCG
GGTAGTGCGCAGGAAGTACAACCAGCTGCATGGTCCGCACTGGGCATTGACGCTGTAGTTGTCGGTCAGGTCACTCCGAA
TCCGGATGGTTCTTACAATGTTGCTTATCAACTTGTTGACACTGGCGGCGCACCGGGTACTGTACTTGCTCAGAACTCGT
ACAAAGTGAACAAGCAGTGGCTGCGTTATGCTGGTCATACCGCCAGTGATGAAGTGTTTGAAAAACTGACCGGCATTAAA
GGTGCGTTCCGTACTCGTATTGCCTACGTTGTTCAGACCAACGGCGGTCAGTTCCCGTATGAACTGCGCGTATCTGACTA
TGACGGTTACAACCAGTTTGTCGTTCACCGTTCACCGCAGCCGCTGATGTCTCCGGCGTGGTCACCAGACGGTTCTAAAC
TGGCTTATGTGACCTTCGAAAGCGGTCGTTCCGCGCTGGTTATTCAGACGCTGGCAAATGGCGCTGTACGTCAGGTGGCT
TCATTCCCGCGTCACAACGGTGCACCTGCATTCTCGCCAGACGGCAGCAAACTGGCATTCGCCTTGTCGAAAACCGGTAG
TCTGAACCTGTACGTAATGGATTTGGCTTCTGGTCAGATCCGCCAGGTGACTGATGGTCGCAGTAACAATACCGAACCGA
CCTGGTTCCCGGATAGCCAGAACCTGGCATTTACTTCTGACCAGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAAC
GGCGGTGCGCCACAACGTATTACCTGGGAAGGTTCGCAGAACCAGGATGCGGATGTCAGCAGCGACGGTAAATTTATGGT
AATGGTCAGCTCCAATGGTGGGCAGCAGCACATTGCCAAACAAGATCTGGCAACGGGAGGCGTACAAGTTCTGTCGTCCA
CGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGATGGGATCCGTG
CTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTC
GCCGTATCTGTGA

                                