Detailed information of regulator

Summary

Regulator ID	REG0250
Regulator type	protein
Regulatory pathway (from literatures)	Global regulator
KEGG pathway (determined by BlastKOALA)	-
Related T6SS	T6SS00464 (Type i4b)
Strain	Escherichia coli 042
Replicon	chromosome
Sequence	Protein sequence (138 a.a.); Nucleotide sequence (414 bp)
Description	PaggR::lacZ reporter plasmids were introduced into an E. coli K12 hns mutant in the presence (pBADaggR) and in the absence (pBAD33) of AggR and further analysis suggested that the H-NS-dependent zone of repression lies downstream of the transcription start site (+114 to +290) as deletion of this DNA fragment eliminated dependence on H-NS.
Reference	[1] Morin N, Tirling C, Ivison SM, et al. Autoactivation of the AggR regulator of enteroaggregative Escherichia coli in vitro and in vivo. FEMS Immunol Med Microbiol. 2010 Apr;58(3):344-55. doi: 10.1111/j.1574-695X.2010.00645.x. Epub 2010 Jan 28. PMID: 20132305

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External database links

Locus tag (Gene)	EC042_RS06800 (Hns)
Coordinates (Strand)	1376831..1377244 (-)
NCBI ID	447210122
RefSeq	NC_017626
Uniprot ID	A0A1Z3V3P7_ECOLX, M9GBT6_ECOLX, A0A0E0Y0E5_ECO1C, S1PGU9_ECOLX, L3CDP9_ECOLX, S1EMQ0_ECOLX, L3Q903_ECOLX, S1I0A0_ECOLX, L4JBG0_ECOLX, U9YV92_ECOLX, U9YIC2_ECOLX, V0STJ3_ECOLX, U9ZPF1_ECOLX, V0VFV5_ECOLX, V0Y7Q8_ECOLX, V0ZC62_ECOLX, V0B017_ECOLX, A0A0E2L702_ECOU3, A0A080J190_ECOLX, A0A029I4E5_ECOLX, A0A0A0F9S4_ECOLX, HNS_ECOL6, D3H2L9_ECO44, Q32GW9_SHIDS, E3PKM4_ECOH1, A7ZL10_ECO24, A0A0E1SRU9_ECOLX, Q1RCK0_ECOUT, A0A0H2YYE1_ECOK1, B6I9V2_ECOSE, B1LH64_ECOSM, A0A140NCH9_ECOBD, J7RQM6_ECOLX, A0A0H3PNM3_ECO5C, D6I752_ECOLX, D6J9K8_ECOLX, F4NGY0_ECOLX, E0IV23_ECOLW, A0A163Y654_KLEOX, B7UQC9_ECO27, B7MKY9_ECO45, B7LHI0_ECO55, B7N450_ECOLU, A0A0H3MG90_ECO7I, B7MU23_ECO81, A0A1S9KFY8_SHIDY, HNS_SHIFL, A0A1W2MMX0_SHIFL, A0A0H7VBX7_SHISO, A0A1X3JJB2_ECOLX, A0A1X3L589_ECOLX, A0A1X3KEK0_ECOLX, F4VBR7_ECOLX, F4SGV7_ECOLX, A0A1X3I9L5_ECOLX, A0A0K9T7Q1_ECOLX, F4TDD1_ECOLX, A0A1X3LIQ7_ECOLX, A0A1X3LWY5_ECOLX, A0A1X3J146_ECOLX, E9XM74_ECOLX, E6BLQ4_ECOLX, E1IMZ1_ECOLX, E1J912_ECOLX, D8E2X7_ECOLX, A0A0H3EJ00_ECO8N, D3QR08_ECOCB, A0A0E1LWR3_ECOLX, D8ACN8_ECOMS, D7XMK7_ECOLX, D7Y5Q5_ECOM1, E9TEY2_ECOLX, HNS_ECOLI, HNS_ECO57, A0A090B484_ECO57, I2T0V9_ECOLX, I2WMZ9_ECOLX, A0A192CMC6_ECOLX, W8ZH67_ECOLX, A0A090BZR8_ECOLX
KEGG ID	ecq:ECED1_1388, elh:ETEC_1341, ecc:c1701, ect:ECIAI39_1572, elw:ECW_m1329, ecj:JW1225, eci:UTI89_C1434, elo:EC042_1292, ecos:EC958_1510, ecv:APECO1_351, eck:EC55989_1332, eok:G2583_1511, ecw:EcE24377A_1386, eco:b1237, ecz:ECS88_1305, ecm:EcSMS35_1904, ebd:ECBD_2385, sfl:SF1237, ecs:ECs1739, ecy:ECSE_1287, esl:O3K_14465, ecg:E2348C_1364, eln:NRG857_06355, sdy:SDY_1289, ece:Z2013, sft:NCTC1_01307, sfx:S1323, eum:ECUMN_1536
KEGG pathway (determined by BlastKOALA)	-
PDB ID	1NI8, 2MW2, 1HNS, 1HNR, 1LR1

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  Target

Target	Regulation (↓/↑)	Target sequence
EC042_RS26480 (aggR) promoter	↑	ATTAACAAACGTATTTTATATGAGTTAAAAATATATCTTTTTATTGATAAGAGTTAGGTCATTCTAACGCAGATTGCCTGATAAAGACATTTTTTTCATGTGAGAATGATATGAAATTAAAACAAAACATCGAAAAAGA

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Pfam domain hit(s) of regulator

Domain	Pfam ID	E-value	Aligned region
Histone_HNS	PF00816.23	4.8e-19	33..127

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Transmembrane helices

• Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-137	MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEESAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDENGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ

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Signal peptides

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9965	-	-

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  Protein sequence of regulator: 138 a.a.    .Download


  Nucleotide sequence of regulator: 414 bp    .Download