Detailed information of Recipient susceptibility factor

Summary

ID	RSF000017
Strain	Escherichia coli BW25113 strain K-12
Replicon	chromosome
Sequence	Protein sequence (202 a.a.); Nucleotide sequence (606 bp)
Response to T6SS attack	protective
Known or putative function	Osmotic stress response
Description	The mutants with more-pronounced lysis, and then, competition assays with serial plating confirmed their survival was significantly reduced by the TseL-active T6SS-dependent killer
Reference	[1] Kamal F, Liang X, Manera K, et al. Differential Cellular Response to Translocated Toxic Effectors and Physical Penetration by the Type VI Secretion System. Cell Rep. 2020 Jun 16;31(11):107766. doi: 10.1016/j.celrep.2020.107766. PMID: 32553162 [2] Hersch SJ, Manera K, Dong TG. Defending against the Type Six Secretion System: beyond Immunity Genes. Cell Rep. 2020 Oct 13;33(2):108259. doi: 10.1016/j.celrep.2020.108259. PMID: 33053336

Experimental investigation has been performed.

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External database links

Locus tag (Gene)	BW25113_RS22785 (osmY)
Coordinates (Strand)	4601213..4601818 (+)
NCBI ID	481041372
RefSeq	NZ_CP009273
Uniprot ID	M9HFF6_ECOLX, K4XMA2_ECOLX, S1NME1_ECOLX, S1E2S3_ECOLX, L3QFJ6_ECOLX, S1IAB8_ECOLX, L4JGW6_ECOLX, U9YUN5_ECOLX, U9XN33_ECOLX, U9ZAB1_ECOLX, V0VF36_ECOLX, V0ZMB5_ECOLX, U9ZQB8_ECOLX, A0A0E2KWX7_ECOU3, A0A080I7J3_ECOLX, A0A0A0F9Z3_ECOLX, OSMY_ECOL6, D3GY15_ECO44, A0A0H2Z6L9_ECOK1, B6I2Q7_ECOSE, J7S064_ECOLX, D6J695_ECOLX, E0J446_ECOLW, A0A170RK33_KLEOX, B7UR03_ECO27, B7MNI2_ECO45, B7NH43_ECOLU, A0A0H3MTB7_ECO7I, A0A1S9KHH9_SHIDY, A0A0H8K5P1_SHISO, E6BJD6_ECOLX, E1J593_ECOLX, D8ECM0_ECOLX, A0A0H3EQV4_ECO8N, D3QND8_ECOCB, A0A0E1M9T8_ECOLX, D7XQP9_ECOLX, D7Y3U9_ECOM1, OSMY_ECOLI, I2SL08_ECOLX, I2UPV4_ECOLX, I2WSE1_ECOLX
KEGG ID	eum:ECUMN_4999, elo:EC042_4872, ecv:APECO1_2005, ecc:c5457, ecg:E2348C_4673, ecj:JW4338, eln:NRG857_22120, ect:ECIAI39_4904, elw:ECW_m4737, ecz:ECS88_5056, eco:b4376, eok:G2583_5236, ecy:ECSE_4650
PDB ID	-

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Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
BON	PF04972.19	3.8e-20	55..122
BON	PF04972.19	9.6e-20	135..199

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Transmembrane helices

• Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-201	MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVDSSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEAVKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVETTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK

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Signal peptides

Prediction	Probability	Cleavage site	Signal peptide sequence
SP(Sec/SPI)	0.9936	pos: 28-29. AYA-EN.	MTMTRLKISKTLLAVMLTSAVATGSAYA

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  Protein sequence: 202 a.a.    .Download


  Nucleotide sequence: 606 bp    .Download