Detailed information of effector


Summary

Effector ID EFF18621 experimental
Effector type Tle
Cognate immunity protein IMU17297 experimental
Related T6SS (Type) T6SS00548 experimental (Type i2)
Strain Klebsiella pneumoniae subsp. pneumoniae HS11286
Replicon chromosome
Sequence Protein sequence (807 a.a.); Nucleotide sequence (2419 bp)
Description WT-V caused a statistically significant impairment/killing of the Δtle1KPΔtli1KP-V mutant, but the Δtle1KP-V mutant did not. Additionally, when the prey (Δtle1KPΔtli1KP-V) was complemented with tli1KP, the inhibition by the attacker WT-V was same to the attacker Δtle1KP-V.
Reference
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed
External database links

Locus tag (Gene) KPHS_23105 (Tle1KP)
Coordinates (Strand) 2341000..2343418 (+)
NCBI ID -
RefSeq NC_016845
Uniprot ID -
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DUF2235 PF09994.11 6.4e-08 65..263
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-781 MSEITETHAAWVPPPFPPQGRLPGRALQVGQNCHQQNSDERRYHQELCLAAGRRVDPPCCKTLHISLFFDGTGNNLNHDFFIANPKHPTNIARLFRATIGTGTAGGVPSDGQSELFDDDAEGDGKYFKFYMPGVGTPFPEVNDPDYSTMGLVGAVKGEDRINWALLRIIDVLMFSATKKWLTTTESRRSLKEMSTSWNRLWFGGSHNRYEEFTRLLNDLASDLKPLIIQPEPGKPKLTGIKLYVYGFSRGAAAARTFVRWLSELLPPPAAEGEKPPQCLQTGGMRLPVSVEFLGLLDTVASVGVAHVVPVADGHMSWADGTMELPDDETYGGLIKKMCSSGLRA*AASLLSAGLGAAGKRQIPALCH*GGLPGDALGYWWRISAGGSRESEW*R*CIIAVADSFERYIYRCF*RRGSIKSTTGYAARVF*K*CLEKNAIRFVRCVFC**GSGQPLQCLARTHSGPNHAENVRSRSSLSLRTTCRRRQSGNRHC*ADGLDHRLAHRPLCQRLDAENALLSARQEYRRAACRA*GGRRGS**KTGGGAQGSPEPDSKPAP*QDG*AGFAAGREGF*PKDGSNPAVRRGEGVR*GLPRRLPYSG*PGAAGAGYRAAAGDFRSQYR**SAGIPAHEA*RRGPGGRPVPRGRGGQQRGTACRAGEGAV**PDT*LTRLVYVCRARHARDVDRLLPLPDDLLQRALQ*TALAAGARRRPGGFCDRDRGCRAQFPAKAPDRQAGRAGCDRRRALAGGGGAGQDNRGSAAGAARRGAAAGIYPRAGNRC


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9605 - -


  Protein sequence: 807 a.a.    .

>EFF18621 NC_016845:2341000-2343418 [Klebsiella pneumoniae subsp. pneumoniae HS11286]
MSEITETHAAWVPPPFPPQGRLPGRALQVGQNCHQQNSDERRYHQELCLAAGRRVDPPCC
KTLHISLFFDGTGNNLNHDFFIANPKHPTNIARLFRATIGTGTAGGVPSDGQSELFDDDA
EGDGKYFKFYMPGVGTPFPEVNDPDYSTMGLVGAVKGEDRINWALLRIIDVLMFSATKKW
LTTTESRRSLKEMSTSWNRLWFGGSHNRYEEFTRLLNDLASDLKPLIIQPEPGKPKLTGI
KLYVYGFSRGAAAARTFVRWLSELLPPPAAEGEKPPQCLQTGGMRLPVSVEFLGLLDTVA
SVGVAHVVPVADGHMSWADGTMELPDDETYGGLIKKMCSSGLRA*AASLLSAGLGAAGKR
QIPALCH*GGLPGDALGYWWRISAGGSRESEW*R*CIIAVADSFERYIYRCF*RRGSIKS
TTGYAARVF*K*CLEKNAIRFVRCVFC**GSGQPLQCLARTHSGPNHAENVRSRSSLSLR
TTCRRRQSGNRHC*ADGLDHRLAHRPLCQRLDAENALLSARQEYRRAACRA*GGRRGS**
KTGGGAQGSPEPDSKPAP*QDG*AGFAAGREGF*PKDGSNPAVRRGEGVR*GLPRRLPYS
G*PGAAGAGYRAAAGDFRSQYR**SAGIPAHEA*RRGPGGRPVPRGRGGQQRGTACRAGE
GAV**PDT*LTRLVYVCRARHARDVDRLLPLPDDLLQRALQ*TALAAGARRRPGGFCDRD
RGCRAQFPAKAPDRQAGRAGCDRRRALAGGGGAGQDNRGSAAGAARRGAAAGIYPRAGNR
CRTAKGAESGRAAGAGASRAAGQLAV*

  Nucleotide sequence: 2419 bp    .

>EFF18621 NC_016845:2341000-2343418 [Klebsiella pneumoniae subsp. pneumoniae HS11286]
ATGTCCGAAATAACTGAAACCCATGCCGCCTGGGTCCCGCCGCCGTTTCCGCCGCAGGGACGTCTGCCGG
GCCGGGCGCTGCAGGTTGGGCAGAACTGCCATCAGCAGAACAGCGACGAGCGGCGCTATCATCAGGAACT
GTGCCTGGCTGCCGGACGGCGCGTGGATCCGCCGTGCTGCAAGACGCTGCATATCAGTCTGTTTTTCGAT
GGTACCGGCAATAACTTAAATCATGACTTTTTTATCGCGAACCCGAAACATCCGACCAATATCGCCCGGC
TGTTTCGGGCGACTATTGGAACGGGTACGGCTGGCGGGGTGCCGTCTGACGGTCAGAGTGAGCTGTTTGA
TGATGACGCCGAGGGGGACGGGAAATATTTTAAATTCTATATGCCTGGCGTTGGCACCCCCTTCCCGGAA
GTGAATGACCCGGACTATTCCACCATGGGTCTGGTGGGGGCAGTTAAGGGGGAGGATCGGATTAACTGGG
CGCTGCTGCGGATAATCGATGTGCTGATGTTTAGTGCCACCAAAAAATGGCTGACCACCACAGAGAGTCG
TCGTTCACTGAAAGAAATGAGCACCAGCTGGAACCGGCTGTGGTTTGGCGGCAGCCATAACCGCTATGAA
GAGTTCACTCGTTTGCTGAATGATTTGGCCTCTGATTTGAAGCCCCTGATTATTCAGCCTGAGCCGGGCA
AGCCGAAACTGACAGGCATCAAGCTGTATGTCTATGGTTTTTCCCGCGGCGCGGCAGCGGCGCGCACCTT
TGTGCGCTGGCTGAGCGAACTGCTGCCCCCGCCGGCGGCGGAGGGTGAAAAACCGCCGCAGTGCCTGCAG
ACAGGCGGGATGCGGCTGCCCGTCAGCGTGGAGTTTCTCGGGCTGCTGGATACGGTGGCCTCCGTCGGGG
TGGCGCATGTGGTGCCGGTTGCCGACGGGCATATGTCCTGGGCAGACGGCACGATGGAGCTGCCGGATGA
TGAAACCTATGGCGGGCTGATAAAAAAAATGTGTTCATCTGGTCTCCGGGCATGAGCAGCGTCTCTGCTT
TCCGCTGGACTCGGTGCGGCGGGCAAACGGCAAATACCCGCCCTGTGCCACTGAGGTGGTTTACCCGGGG
ATGCACTCGGATATTGGTGGAGGATATCCGCCGGGGGATCAAGGGAAAGCGAATGGTAAAGATGATGCAT
TATTGCTGTCGCAGATTCCTTTGAACGATATATATACCGCTGCTTTTAGCGCAGGGGCTCCATTAAAAGT
ACCACAGGATACGCTGCCAGAGTTTTTTAAAAATGATGCCTGGAGAAAAATGCCATTAGATTTGTTAGAT
GCGTTTTTTGTTGATGAGGCTCTGGTCAACCGCTTCAATGCCTGGCGCGAACTCACTCTGGGCCAAACCA
CGCCGAAAACGTTCGATCCCGAAGCAGCCTCTCACTACGAACCACCTGCCGCCGGCGGCAGTCTGGAAAC
CGTCATTGCTGAGCAGATGGCCTGGATCACCGCCTGGCGCATCGACCGCTATGCCAGAGGCTCGATGCTG
AAAACGCCCTTCTATCAGCGCGCCAAGAATACCGACGCGCTGCCTGCCGCGCGTAAGGCGGCAGAAGAGG
TTCGTGATGAAAAACAGGCGGCGGTGCTCAGGGCTCGCCAGAACCAGATAGCAAACCAGCCCCCTGACAG
GATGGATGAGCTGGTTTTGCAGCCGGGCGTGAAGGATTTTGACCCAAAGATGGATCAAACCCAGCTGTTC
GACGCGGCGAAGGAGTTCGGTAAGGATTACCACGACGGTTACCGTATTCCGGATAACCTGGCGCAGCTGG
TGCTGGATACCGTGCTGCAGCCGGTGATTTTCGTTCTCAATACCGATGATGAAGCGCAGGAATACCGGCG
CATGAAGCGTGACGGAGAGGCCCGGGTGGTCGTCCTGTTCCCAGAGGCCGGGGAGGCCAGCAACGCGGAA
CAGCCTGCCGGGCTGGTGAGGGCGCTGTTTGATGACCAGATACATGACTCACGCGCCTGGTTTATGTATG
CCGCGCTCGGCACGCGCGAGATGTGGACCGGTTACTTCCGCTACCGGATGATTTACTTCAGCGAGCGCTG
CAGTAAACCGCTCTCGCCGCTGGTGCTCGCCGGAGACCTGGTGGGTTTTGCGACCGTGACCGCGGGTGTC
GTGCTCAGTTTCCGGCAAAAGCGCCTGACCGGCAAGCTGGCCGGGCTGGCTGCGACAGGCGCCGTGCGCT
CGCTGGAGGTGGCGGTGCTGGACAAGATAACCGGGGAAGCGCTGCCGGAGCTGCCCGGAGGGGCGCAGCT
GCGGGCATTTACCCACGAGCCGGGAACCGTTGTCGCACAGCAAAAGGCGCGGAAAGCGGAAGAGCAGCTG
GCGCGGGGGCAAGCCGCGCTGCCGGCCAGCTGGCTGTAG