Detailed information of T6SS
Note: only experimentally validated proteins are displayed in the graph.
Summary
| T6SS ID | T6SS00548  |
| T6SS type | Type i2 |
| Strain | Klebsiella pneumoniae subsp. pneumoniae HS11286 |
| Replicon (RefSeq) | chromosome (NC_016845) |
| Location | 2326237..2353260 |
| Function | anti-bacterial |
| Comment | Hcp was present only in the supernatant of the wild type strain but not in ΔvipA mutant.WT-V caused a statistically significant impairment/killing of the Δtle1KPΔtli1KP-V mutant, but the Δtle1KP-V mutant did not. Additionally, when the prey (Δtle1KPΔtli1KP-V) was complemented with tli1KP, the inhibition by the attacker WT-V was same to the attacker Δtle1KP-V. |
| References | [1] Liu L, Ye M, Li X, Li J, Deng Z, Yao YF, Ou HY. et al (2017) Identification and Characterization of an Antibacterial Type VI Secretion System in the Carbapenem-Resistant Strain Klebsiella pneumoniae HS11286.. Front Cell Infect Microbiol. 0.598611111. [PudMed:29085808] |
Experimental investigation has been performed.T6SS components and genome coordinates
| Component | PAAR | TagL | TssA | TssB | TssC | TssD | TssF | TssG | TssH | TssI | TssJ | TssK | TssL | TssM |
| Number | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| Component ID | Component | Locus tag (Gene) | Coordinate | Strand | Size (bp) | NCBI ID | Product |
|---|---|---|---|---|---|---|---|
| KPHS_22930 | 2322412..2322621 | + | 210 | YP_005226593.1 | hypothetical protein | ||
| KPHS_22940 | 2323071..2324558 | + | 1488 | YP_005226594.1 | putative reverse transcriptase/maturase | ||
| KPHS_22950 | 2325143..2325391 | - | 249 | YP_005226595.1 | DinI family protein | ||
| KPHS_22960 | 2325956..2326078 | + | 123 | YP_005226596.1 | hypothetical protein | ||
| T6CP005798 | TssB | KPHS_22970 | 2326237..2326728 | + | 492 | YP_005226597.1 | type VI secretion system contractile sheath small subunit |
| T6CP005799 | TssC | KPHS_22980 | 2326771..2328315 | + | 1545 | YP_005226598.1 | EvpB/family type VI secretion protein |
| T6CP005800 | TssK | KPHS_22990 | 2328325..2329668 | + | 1344 | YP_005226599.1 | type VI secretion system baseplate subunit TssK |
| T6CP008981 | TssL | KPHS_23000 | 2329665..2330354 | + | 690 | YP_005226600.1 | DotU family type IV/VI secretion system protein |
| T6CP011987 | TagL | KPHS_23010 | 2330351..2332057 | + | 1707 | YP_005226601.1 | OmpA/MotB domain-containing protein |
| T6CP008982 | TssD | KPHS_23020 | 2332062..2332553 | + | 492 | YP_005226602.1 | Hcp family type VI secretion system effector |
| T6CP005801 | TssH | KPHS_23030 | 2332818..2335472 | + | 2655 | YP_005226603.1 | putative protease |
| T6CP005802 | TssI | KPHS_23040 | 2335474..2337843 | + | 2370 | YP_005226604.1 | Rhs element Vgr protein |
| KPHS_23050 | 2337844..2338623 | + | 780 | YP_005226605.1 | DUF4123 domain-containing protein | ||
| KPHS_23060 | 2338687..2339217 | + | 531 | YP_005226606.1 | putative lipoprotein | ||
| KPHS_23070 | 2339286..2339816 | + | 531 | YP_005226607.1 | putative lipoprotein | ||
| KPHS_23080 | 2339884..2340414 | + | 531 | YP_005226608.1 | putative lipoprotein | ||
| KPHS_23090 | 2340482..2341012 | + | 531 | YP_005226609.1 | putative lipoprotein | ||
| KPHS_23100 | 2341000..2342034 | + | 1035 | YP_005226610.1 | DUF2235 domain-containing protein | ||
| KPHS_23110 | 2342120..2343418 | + | 1299 | YP_005226611.1 | hypothetical protein | ||
| T6CP011986 | PAAR | KPHS_23120 | 2343463..2343720 | + | 258 | YP_005226612.1 | PaaR repeat-containing protein |
| KPHS_23130 | 2343717..2344856 | + | 1140 | YP_005226613.1 | hypothetical protein | ||
| T6CP005803 | TssM | KPHS_23140 | 2344840..2348265 | + | 3426 | YP_005226614.1 | ImcF domain-containing protein |
| T6CP009367 | TssA | KPHS_23150 | 2348265..2348672 | + | 408 | YP_005226615.1 | type VI secretion-associated protein |
| KPHS_23160 | 2348724..2349857 | + | 1134 | YP_005226616.1 | type VI secretion-associated protein | ||
| T6CP005804 | TssF | KPHS_23170 | 2349937..2351691 | + | 1755 | YP_005226617.1 | type VI secretion system baseplate subunit TssF |
| T6CP005805 | TssG | KPHS_23180 | 2351655..2352740 | + | 1086 | YP_005226618.1 | type VI secretion system baseplate subunit TssG |
| T6CP005806 | TssJ | KPHS_23190 | 2352718..2353260 | + | 543 | YP_005226619.1 | type VI secretion system lipoprotein TssJ |
| KPHS_23200 | 2353231..2353359 | - | 129 | YP_005226620.1 | hypothetical protein | ||
| KPHS_23210 | 2353389..2353883 | + | 495 | YP_005226621.1 | hypothetical protein | ||
| KPHS_23220 | 2353950..2354663 | - | 714 | YP_005226622.1 | oxidoreductase | ||
| KPHS_23230 | 2354737..2355330 | + | 594 | YP_005226623.1 | putative regulatory protein TetR | ||
| KPHS_23240 | 2355335..2356090 | - | 756 | YP_005226624.1 | putative dehydrogenase/reductase oxidoreductase protein | ||
| KPHS_23250 | 2356249..2357127 | + | 879 | YP_005226625.1 | putative ARAC-type regulatory protein | ||
| KPHS_23260 | 2357178..2357384 | - | 207 | YP_005226626.1 | DUF1471 domain-containing protein |
Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files
Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
Experimental investigation has been performed.
Bioinformatics investigation has been performed.| Regulator ID | Locus tag (Gene) | Regulator type | Regulatory pathway | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Target (Regulation) |
|---|
Experimental investigation has been performed. Bioinformatics investigation has been performed.| Effector ID | Locus tag (Gene) | Classification | Cognate immunity protein | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
|---|---|---|---|---|---|---|---|
| EFF18621 |
KPHS_23105 (Tle1KP) | Tle | IMU17297 |
Klebsiella pneumoniae subsp. pneumoniae HS11286 | chromosome (NC_016845) | 2341000..2343418 (+) | - |
Experimental investigation has been performed. Bioinformatics investigation has been performed.| Immunity protein ID | Locus tag (Gene) | Cognate effector | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
|---|---|---|---|---|---|---|
| IMU17297 |
KPHS_23060 (Tli1KP) | EFF18621 |
Klebsiella pneumoniae subsp. pneumoniae HS11286 | chromosome (NC_016845) | 2338687..2339217 (+) | YP_005226606.1 |
| IMU17298 |
KPHS_23070 (Tli2KP) | EFF18621 |
Klebsiella pneumoniae subsp. pneumoniae HS11286 | chromosome (NC_016845) | 2339286..2339816 (+) | YP_005226607.1 |
| IMU17299 |
KPHS_23080 (Tli3KP) | EFF18621 |
Klebsiella pneumoniae subsp. pneumoniae HS11286 | chromosome (NC_016845) | 2339884..2340414 (+) | YP_005226608.1 |
| IMU17300 |
KPHS_23090 (Tli4KP) | EFF18621 |
Klebsiella pneumoniae subsp. pneumoniae HS11286 | chromosome (NC_016845) | 2340482..2341012 (+) | YP_005226609.1 |
Experimental investigation has been performed.| Accessory protein ID | Locus tag (Gene) | Type | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Related structure protein | Related effector |
|---|