SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP005805
Component protein type	TssG
T6SS ID (Type)	T6SS00548 (Type i2)
Strain	Klebsiella pneumoniae subsp. pneumoniae HS11286
Replicon	chromosome
Sequence	Protein sequence (362 a.a.); Nucleotide sequence (1086 bp)
Reference	[1] Liu L, Ye M, Li X, et al. Identification and Characterization of an Antibacterial Type VI Secretion System in the Carbapenem-Resistant Strain Klebsiella pneumoniae HS11286. Front Cell Infect Microbiol. 2017 Oct 12;7:442. doi: 10.3389/fcimb.2017.00442. eCollection 2017. PMID: 29085808

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	KPHS_23180
Coordinate (Strand)	2351655..2352740 (+)
NCBI ID	YP_005226618.1
RefSeq	NC_016845
Uniprot ID	A0A0H3GSH3_KLEPH
KEGG ID	kpm:KPHS_23180
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssG	PF06996.13	5.7e-63	21..320

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-361	MGREAQPPHSRLTPRLEADLPRINFYRFCQLLEKRRPGQPLMGGTSHPADDPVRFYPHPGMGFPASELKAVEYDEADDSRPPVIRTTFMGLYGVDSPLPTAYLDDIAQHREGHEALQGLLDIFSHRIMTQFYRIWRKYSWPATFEPGGTDRLSQSLLGLAGLGIPGTTQHIASPASRFLALTGVLRQPGKTQQGIQALVTLLAPETTVRVSPYSLRPVAISQPLGFYGDDDFFLDGNTPLGDEAMDASSQLLVALTTDNPAEAQGWKPDGPLFRDFLILLRVYLGWRFRANITLTAPTRLLAVPPLGDEPFWLGMNGVLGVGDGESEGDIPQTFTTELGTYTGLQPATFLQGNRRVTYKFD

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9812	-	-

Protein sequence: 362 a.a.

						>T6CP005805 NC_016845:2351655-2352740 [Klebsiella pneumoniae subsp. pneumoniae HS11286] [TssG]
MGREAQPPHSRLTPRLEADLPRINFYRFCQLLEKRRPGQPLMGGTSHPADDPVRFYPHPGMGFPASELKAVEYDEADDSR
PPVIRTTFMGLYGVDSPLPTAYLDDIAQHREGHEALQGLLDIFSHRIMTQFYRIWRKYSWPATFEPGGTDRLSQSLLGLA
GLGIPGTTQHIASPASRFLALTGVLRQPGKTQQGIQALVTLLAPETTVRVSPYSLRPVAISQPLGFYGDDDFFLDGNTPL
GDEAMDASSQLLVALTTDNPAEAQGWKPDGPLFRDFLILLRVYLGWRFRANITLTAPTRLLAVPPLGDEPFWLGMNGVLG
VGDGESEGDIPQTFTTELGTYTGLQPATFLQGNRRVTYKFD*					

Nucleotide sequence: 1086 bp

						>T6CP005805 NC_016845:2351655-2352740 [Klebsiella pneumoniae subsp. pneumoniae HS11286] [TssG]
ATGGGAAGAGAAGCACAGCCGCCGCATTCCCGGCTGACCCCGCGACTGGAGGCCGACCTGCCGCGGATCAATTTTTACCG
CTTCTGCCAGCTTCTGGAAAAACGGCGTCCCGGCCAACCACTGATGGGGGGAACCAGCCATCCGGCTGACGATCCGGTGC
GTTTTTACCCGCATCCGGGAATGGGATTTCCCGCCAGCGAACTGAAAGCCGTGGAATACGATGAGGCCGATGACAGCAGG
CCGCCGGTCATTCGCACCACCTTTATGGGGTTGTACGGTGTCGATTCGCCGCTCCCGACGGCATATCTCGATGATATCGC
CCAGCACAGGGAAGGGCATGAAGCGCTGCAGGGCTTACTGGATATCTTCAGCCACCGGATCATGACGCAGTTTTACCGCA
TCTGGCGGAAATACTCCTGGCCCGCCACCTTTGAGCCCGGCGGCACCGATCGTCTCTCGCAGTCGCTACTGGGCCTGGCG
GGGCTAGGCATCCCCGGCACGACGCAGCATATCGCCTCGCCGGCGTCGCGCTTTCTGGCGCTAACCGGCGTGCTGCGTCA
GCCGGGCAAAACCCAGCAGGGGATCCAGGCGCTGGTCACCCTGCTGGCCCCGGAGACCACCGTCAGGGTGAGCCCGTACA
GCCTGCGTCCGGTGGCGATCAGCCAGCCGCTGGGGTTTTACGGCGATGACGACTTCTTCCTGGACGGCAATACGCCGCTG
GGTGACGAAGCGATGGATGCCAGCAGCCAGCTCCTCGTCGCCCTTACCACCGACAATCCCGCGGAGGCGCAGGGCTGGAA
GCCTGACGGCCCGCTGTTTCGGGACTTTCTCATCCTGCTGCGGGTATACCTCGGCTGGCGATTCCGGGCCAACATCACCC
TGACCGCGCCGACCCGACTGCTGGCTGTCCCGCCGCTGGGGGACGAGCCGTTCTGGCTCGGTATGAACGGCGTGCTGGGC
GTGGGTGATGGCGAGAGTGAGGGCGATATTCCTCAGACCTTTACCACCGAGCTGGGTACCTATACGGGCCTGCAGCCTGC
CACCTTTCTACAAGGAAACCGACGTGTTACGTATAAGTTTGACTAA					