Detailed information of immunity protein


Summary

Immunity protein ID IMU17298 experimental
Cognate effector EFF18621 experimental
Related T6SS (Type) T6SS00548 experimental (Type i2)
Strain Klebsiella pneumoniae subsp. pneumoniae HS11286
Replicon chromosome
Sequence Protein sequence (177 a.a.); Nucleotide sequence (531 bp)
Description WT-V caused a statistically significant impairment/killing of the Δtle1KPΔtli1KP-V mutant, but the Δtle1KP-V mutant did not. Additionally, when the prey (Δtle1KPΔtli1KP-V) was complemented with tli1KP, the inhibition by the attacker WT-V was same to the attacker Δtle1KP-V.
Reference
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed

External database links

Locus tag (Gene) KPHS_23070 (Tli2KP)
Coordinates (Strand) 2339286..2339816 (+)
NCBI ID YP_005226607.1
RefSeq NC_016845
Uniprot ID A0A0H3GMC7_KLEPH
KEGG ID kpm:KPHS_23070
PDB ID -

Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DUF3304 PF11745.10 2.7e-40 37..148

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-176 MKSALISPLLAGLLLLTGCAQPAAQAGGGGGGTIKAINHTKWAINHFSVNGQSGIDIIGPFQGGGGGCCFSVPARWTPGMTVRVDWESGEASTEGFPGFADYEKYKAWEKKMSSQNRQHSKTVPLPDYNGQDVCGITVHFLPCDDVKVTTSCWSPRNVNYPIKEPVRMKEPAVCPK



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
LIPO(Sec/SPII) 0.8852 pos: 18-19. LTG-CA. MKSALISPLLAGLLLLTG



  Protein sequence: 177 a.a.    .

>IMU17298 NC_016845:2339286-2339816 [Klebsiella pneumoniae subsp. pneumoniae HS11286]
MKSALISPLLAGLLLLTGCAQPAAQAGGGGGGTIKAINHTKWAINHFSVNGQSGIDIIGPFQGGGGGCCFSVPARWTPGM
TVRVDWESGEASTEGFPGFADYEKYKAWEKKMSSQNRQHSKTVPLPDYNGQDVCGITVHFLPCDDVKVTTSCWSPRNVNY
PIKEPVRMKEPAVCPK*

  Nucleotide sequence: 531 bp    .

>IMU17298 NC_016845:2339286-2339816 [Klebsiella pneumoniae subsp. pneumoniae HS11286]
ATGAAATCTGCATTAATCAGCCCGCTGCTGGCGGGTCTGCTGCTGTTAACCGGCTGCGCGCAGCCTGCCGCACAGGCCGG
AGGCGGCGGTGGCGGAACAATAAAAGCCATCAACCACACCAAATGGGCGATCAACCACTTCAGTGTCAACGGCCAGTCGG
GGATCGACATTATAGGTCCGTTTCAGGGCGGCGGTGGCGGCTGCTGCTTCAGCGTGCCCGCCCGCTGGACGCCGGGTATG
ACGGTGCGCGTTGACTGGGAAAGTGGTGAGGCATCGACTGAAGGTTTTCCTGGATTTGCTGATTACGAAAAATATAAAGC
CTGGGAGAAAAAAATGAGCTCTCAGAATCGGCAGCACAGCAAAACCGTTCCGTTGCCCGACTATAACGGCCAGGATGTGT
GCGGCATTACGGTGCACTTTTTACCCTGCGATGACGTGAAGGTGACCACCAGCTGCTGGTCACCGAGAAATGTGAACTAT
CCGATAAAAGAGCCCGTCAGGATGAAGGAGCCCGCCGTATGTCCGAAGTAG