Detailed information of immunity protein
Summary
| Immunity protein ID | IMU17300  |
| Cognate effector | EFF18621 |
| Related T6SS (Type) | T6SS00548 (Type i2) |
| Strain | Klebsiella pneumoniae subsp. pneumoniae HS11286 |
| Replicon | chromosome |
| Sequence | Protein sequence (177 a.a.); Nucleotide sequence (531 bp) |
| Description | WT-V caused a statistically significant impairment/killing of the Δtle1KPΔtli1KP-V mutant, but the Δtle1KP-V mutant did not. Additionally, when the prey (Δtle1KPΔtli1KP-V) was complemented with tli1KP, the inhibition by the attacker WT-V was same to the attacker Δtle1KP-V. |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | KPHS_23090 (Tli4KP) |
| Coordinates (Strand) | 2340482..2341012 (+) |
| NCBI ID | YP_005226609.1 |
| RefSeq | NC_016845 |
| Uniprot ID | A0A0H3GRZ7_KLEPH |
| KEGG ID | kpm:KPHS_23090 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| DUF3304 | PF11745.10 | 1.6e-39 | 37..148 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-176 | MKSALISPLLAGLLLLTGCAQPAAQAGGGGGGTIKAINHTKWAINHFSVNGQSGIDSIGPFQGGGGGCCFSVPARWTPGMTVRVEWETGQGSSAGFPGFADRAKYKAWIADIDAQKRQHSQTVPLPDYNGQDVCGITVHFLPCDDVKVTTSCWSPRNANYPIKEPVRMKEPAVCPK |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| LIPO(Sec/SPII) | 0.8858 | pos: 18-19. LTG-CA. | MKSALISPLLAGLLLLTG |
Protein sequence: 177 a.a. .
>IMU17300 NC_016845:2340482-2341012 [Klebsiella pneumoniae subsp. pneumoniae HS11286]
MKSALISPLLAGLLLLTGCAQPAAQAGGGGGGTIKAINHTKWAINHFSVNGQSGIDSIGPFQGGGGGCCFSVPARWTPGM
TVRVEWETGQGSSAGFPGFADRAKYKAWIADIDAQKRQHSQTVPLPDYNGQDVCGITVHFLPCDDVKVTTSCWSPRNANY
PIKEPVRMKEPAVCPK*
MKSALISPLLAGLLLLTGCAQPAAQAGGGGGGTIKAINHTKWAINHFSVNGQSGIDSIGPFQGGGGGCCFSVPARWTPGM
TVRVEWETGQGSSAGFPGFADRAKYKAWIADIDAQKRQHSQTVPLPDYNGQDVCGITVHFLPCDDVKVTTSCWSPRNANY
PIKEPVRMKEPAVCPK*
Nucleotide sequence: 531 bp .
>IMU17300 NC_016845:2340482-2341012 [Klebsiella pneumoniae subsp. pneumoniae HS11286]
ATGAAATCTGCATTAATCAGCCCGCTGCTGGCGGGTCTGCTGCTGTTAACCGGCTGTGCACAGCCAGCCGCACAGGCCGG
AGGCGGCGGTGGCGGCACAATAAAAGCCATCAACCACACAAAATGGGCAATTAACCACTTCAGTGTCAACGGCCAGTCGG
GCATCGATTCAATAGGCCCGTTCCAGGGGGGAGGTGGCGGGTGCTGCTTCAGCGTGCCCGCCCGCTGGACGCCGGGTATG
ACGGTGCGCGTTGAATGGGAAACCGGGCAGGGTTCATCCGCGGGTTTCCCGGGGTTTGCCGACAGGGCTAAATACAAAGC
CTGGATAGCAGACATTGATGCCCAAAAACGCCAGCACAGCCAAACCGTTCCGCTGCCCGACTATAACGGCCAGGATGTGT
GCGGCATTACGGTGCACTTTTTACCCTGCGATGACGTGAAAGTGACCACCAGCTGCTGGTCACCGAGAAATGCGAATTAT
CCGATAAAAGAGCCCGTCAGGATGAAGGAGCCCGCCGTATGTCCGAAATAA
ATGAAATCTGCATTAATCAGCCCGCTGCTGGCGGGTCTGCTGCTGTTAACCGGCTGTGCACAGCCAGCCGCACAGGCCGG
AGGCGGCGGTGGCGGCACAATAAAAGCCATCAACCACACAAAATGGGCAATTAACCACTTCAGTGTCAACGGCCAGTCGG
GCATCGATTCAATAGGCCCGTTCCAGGGGGGAGGTGGCGGGTGCTGCTTCAGCGTGCCCGCCCGCTGGACGCCGGGTATG
ACGGTGCGCGTTGAATGGGAAACCGGGCAGGGTTCATCCGCGGGTTTCCCGGGGTTTGCCGACAGGGCTAAATACAAAGC
CTGGATAGCAGACATTGATGCCCAAAAACGCCAGCACAGCCAAACCGTTCCGCTGCCCGACTATAACGGCCAGGATGTGT
GCGGCATTACGGTGCACTTTTTACCCTGCGATGACGTGAAAGTGACCACCAGCTGCTGGTCACCGAGAAATGCGAATTAT
CCGATAAAAGAGCCCGTCAGGATGAAGGAGCCCGCCGTATGTCCGAAATAA