Detailed information of effector
Summary
| Effector ID | EFF01523  |
| Effector type | Tde |
| Cognate immunity protein | - |
| Related T6SS (Type) | T6SS00944 (Type Unknown) |
| Strain | Escherichia coli strain PE086 |
| Replicon | chromosome |
| Sequence | Protein sequence (117 a.a.); Nucleotide sequence (351 bp) |
| Description | Hcp-ET4 is functional with colicin-DNase toxin domin |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | het2 (Hcp-ET4) |
| Coordinates (Strand) | 1508..1858 (+) |
| NCBI ID | 1062685124 |
| GenBank | KX118289 |
| Uniprot ID | A0A1C9CJM3_ECOLX |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-116 | MSVSNNGNGAPIPAHIVDKLRGREFKTFDEFREALWLEVSQDPELIAQFSEINQLRISQGFTPFAPDEGHYIGPKETLKKFQIHHFIAIEYGGGVYDIDNLRIVTPRLHDEIHYRR |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9932 | - | - |
Protein sequence: 117 a.a. .
>EFF01523 KX118289:1508-1858 Type VI DNase and RNase Effector (Tde) [Escherichia coli]
MSVSNNGNGAPIPAHIVDKLRGREFKTFDEFREALWLEVSQDPELIAQFSEINQLRISQGFTPFAPDEGHYIGPKETLKK
FQIHHFIAIEYGGGVYDIDNLRIVTPRLHDEIHYRR*
MSVSNNGNGAPIPAHIVDKLRGREFKTFDEFREALWLEVSQDPELIAQFSEINQLRISQGFTPFAPDEGHYIGPKETLKK
FQIHHFIAIEYGGGVYDIDNLRIVTPRLHDEIHYRR*
Nucleotide sequence: 351 bp .
>EFF01523 KX118289:1508-1858 Type VI DNase and RNase Effector (Tde) [Escherichia coli]
ATGTCGGTATCCAATAACGGCAACGGCGCACCAATCCCGGCGCATATTGTTGATAAATTGCGCGGACGGGAGTTTAAAAC
CTTTGATGAGTTTCGCGAGGCGCTGTGGCTGGAGGTGAGTCAGGATCCGGAGTTGATAGCGCAGTTTTCGGAAATTAATC
AGTTACGTATTAGTCAGGGCTTTACACCTTTTGCCCCCGACGAAGGACACTATATTGGTCCTAAAGAAACACTTAAAAAA
TTTCAGATACATCATTTTATTGCTATTGAATATGGCGGCGGCGTATACGATATTGATAATTTACGAATTGTTACCCCCAG
ACTACACGATGAGATTCACTACAGGAGATAA
ATGTCGGTATCCAATAACGGCAACGGCGCACCAATCCCGGCGCATATTGTTGATAAATTGCGCGGACGGGAGTTTAAAAC
CTTTGATGAGTTTCGCGAGGCGCTGTGGCTGGAGGTGAGTCAGGATCCGGAGTTGATAGCGCAGTTTTCGGAAATTAATC
AGTTACGTATTAGTCAGGGCTTTACACCTTTTGCCCCCGACGAAGGACACTATATTGGTCCTAAAGAAACACTTAAAAAA
TTTCAGATACATCATTTTATTGCTATTGAATATGGCGGCGGCGTATACGATATTGATAATTTACGAATTGTTACCCCCAG
ACTACACGATGAGATTCACTACAGGAGATAA