Detailed information of Recipient susceptibility factor
Summary
| ID | RSF000005  |
| Strain | Escherichia coli BW25113 strain K-12 |
| Replicon | chromosome |
| Sequence | Protein sequence (429 a.a.); Nucleotide sequence (1287 bp) |
| Response to T6SS attack | protective |
| Known or putative function | Menbtane integrity |
| Description | The mutants with more-pronounced lysis, and then, competition assays with serial plating confirmed their survival was significantly reduced by the TseL-active T6SS-dependent killer |
| Reference | [1] Kamal F, Liang X, Manera K, et al. Differential Cellular Response to Translocated Toxic Effectors and Physical Penetration by the Type VI Secretion System. Cell Rep. 2020 Jun 16;31(11):107766. doi: 10.1016/j.celrep.2020.107766. PMID: 32553162 [2] Hersch SJ, Manera K, Dong TG. Defending against the Type Six Secretion System: beyond Immunity Genes. Cell Rep. 2020 Oct 13;33(2):108259. doi: 10.1016/j.celrep.2020.108259. PMID: 33053336 |
Experimental investigation has been performed.External database links
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| SurA_N | PF09312.13 | 2.2e-46 | 25..142 |
| Rotamase_3 | PF13616.8 | 1.4e-26 | 281..383 |
| Rotamase | PF00639.23 | 5.8e-21 | 178..272 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-428 | MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDGLMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIANIAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESLAQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIAHSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGESKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGSANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAAQKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| SP(Sec/SPI) | 0.9973 | pos: 20-21. SFA-AP. | MKNWKTLLLGIAMIANTSFA |
Protein sequence: 429 a.a. .
>RSF000005 NZ_CP009273:c54702-53416 [Escherichia coli BW25113]
MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDGLMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQII
LQMGQKMGVKISDEQLDQAIANIAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIAHSADQQALNGGQMGWGRIQELP
GIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGESKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTT
FAAAAKEFSQDPGSANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAAQKDRAYRM
LMNRKFSEEAASWMQEQRASAYVKILSN*
MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDGLMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQII
LQMGQKMGVKISDEQLDQAIANIAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIAHSADQQALNGGQMGWGRIQELP
GIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGESKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTT
FAAAAKEFSQDPGSANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAAQKDRAYRM
LMNRKFSEEAASWMQEQRASAYVKILSN*
Nucleotide sequence: 1287 bp .
>RSF000005 NZ_CP009273:c54702-53416 [Escherichia coli BW25113]
ATGAAGAACTGGAAAACGCTGCTTCTCGGTATCGCCATGATCGCGAATACCAGTTTCGCTGCCCCCCAGGTAGTCGATAA
AGTCGCAGCCGTCGTCAATAACGGCGTCGTGCTGGAAAGCGACGTTGATGGATTAATGCAGTCGGTAAAACTGAACGCTG
CTCAGGCAAGGCAGCAACTTCCTGATGACGCGACGCTGCGCCACCAAATCATGGAACGTTTGATCATGGATCAAATCATC
CTGCAGATGGGGCAGAAAATGGGAGTGAAAATCTCCGATGAGCAGCTGGATCAGGCGATTGCTAACATCGCGAAACAGAA
CAACATGACGCTGGATCAGATGCGCAGCCGTCTGGCTTACGATGGACTGAACTACAACACCTATCGTAACCAGATCCGCA
AAGAGATGATTATCTCTGAAGTGCGTAACAACGAGGTGCGTCGTCGCATCACCATCCTGCCGCAGGAAGTCGAATCCCTG
GCGCAGCAGGTGGGTAACCAAAACGACGCCAGCACTGAGCTGAACCTGAGCCACATCCTGATCCCGCTGCCGGAAAACCC
GACCTCTGATCAGGTGAACGAAGCGGAAAGCCAGGCGCGCGCCATTGTCGATCAGGCGCGTAACGGCGCTGATTTCGGTA
AGCTGGCGATTGCTCATTCTGCCGACCAGCAGGCGCTGAACGGCGGCCAGATGGGCTGGGGCCGTATTCAGGAGTTGCCC
GGGATCTTCGCCCAGGCATTAAGCACCGCGAAGAAAGGCGACATTGTTGGCCCGATTCGTTCCGGCGTTGGCTTCCATAT
TCTGAAAGTTAACGACCTGCGCGGCGAAAGCAAAAATATCTCGGTGACCGAAGTTCATGCTCGCCATATTCTGCTGAAAC
CGTCGCCGATCATGACTGACGAACAGGCCCGTGTGAAACTGGAACAGATTGCTGCTGATATCAAGAGTGGTAAAACGACT
TTTGCTGCCGCAGCGAAAGAGTTCTCTCAGGATCCAGGCTCTGCTAACCAGGGCGGCGATCTCGGCTGGGCTACACCAGA
TATTTTCGATCCGGCCTTCCGTGACGCCCTGACTCGCCTGAACAAAGGTCAAATGAGTGCACCGGTTCACTCTTCATTCG
GCTGGCATTTAATCGAACTGCTGGATACCCGTAATGTCGATAAAACCGACGCTGCGCAGAAAGATCGTGCATACCGCATG
CTGATGAACCGTAAGTTCTCGGAAGAAGCAGCAAGCTGGATGCAGGAACAACGTGCCAGCGCCTACGTTAAAATCCTGAG
CAACTAA
ATGAAGAACTGGAAAACGCTGCTTCTCGGTATCGCCATGATCGCGAATACCAGTTTCGCTGCCCCCCAGGTAGTCGATAA
AGTCGCAGCCGTCGTCAATAACGGCGTCGTGCTGGAAAGCGACGTTGATGGATTAATGCAGTCGGTAAAACTGAACGCTG
CTCAGGCAAGGCAGCAACTTCCTGATGACGCGACGCTGCGCCACCAAATCATGGAACGTTTGATCATGGATCAAATCATC
CTGCAGATGGGGCAGAAAATGGGAGTGAAAATCTCCGATGAGCAGCTGGATCAGGCGATTGCTAACATCGCGAAACAGAA
CAACATGACGCTGGATCAGATGCGCAGCCGTCTGGCTTACGATGGACTGAACTACAACACCTATCGTAACCAGATCCGCA
AAGAGATGATTATCTCTGAAGTGCGTAACAACGAGGTGCGTCGTCGCATCACCATCCTGCCGCAGGAAGTCGAATCCCTG
GCGCAGCAGGTGGGTAACCAAAACGACGCCAGCACTGAGCTGAACCTGAGCCACATCCTGATCCCGCTGCCGGAAAACCC
GACCTCTGATCAGGTGAACGAAGCGGAAAGCCAGGCGCGCGCCATTGTCGATCAGGCGCGTAACGGCGCTGATTTCGGTA
AGCTGGCGATTGCTCATTCTGCCGACCAGCAGGCGCTGAACGGCGGCCAGATGGGCTGGGGCCGTATTCAGGAGTTGCCC
GGGATCTTCGCCCAGGCATTAAGCACCGCGAAGAAAGGCGACATTGTTGGCCCGATTCGTTCCGGCGTTGGCTTCCATAT
TCTGAAAGTTAACGACCTGCGCGGCGAAAGCAAAAATATCTCGGTGACCGAAGTTCATGCTCGCCATATTCTGCTGAAAC
CGTCGCCGATCATGACTGACGAACAGGCCCGTGTGAAACTGGAACAGATTGCTGCTGATATCAAGAGTGGTAAAACGACT
TTTGCTGCCGCAGCGAAAGAGTTCTCTCAGGATCCAGGCTCTGCTAACCAGGGCGGCGATCTCGGCTGGGCTACACCAGA
TATTTTCGATCCGGCCTTCCGTGACGCCCTGACTCGCCTGAACAAAGGTCAAATGAGTGCACCGGTTCACTCTTCATTCG
GCTGGCATTTAATCGAACTGCTGGATACCCGTAATGTCGATAAAACCGACGCTGCGCAGAAAGATCGTGCATACCGCATG
CTGATGAACCGTAAGTTCTCGGAAGAAGCAGCAAGCTGGATGCAGGAACAACGTGCCAGCGCCTACGTTAAAATCCTGAG
CAACTAA