SecReT6, T6SS database

Detailed information of Recipient susceptibility factor

Summary

ID	RSF000011
Strain	Escherichia coli BW25113 strain K-12
Replicon	chromosome
Sequence	Protein sequence (512 a.a.); Nucleotide sequence (1536 bp)
Response to T6SS attack	protective
Known or putative function	Acid stress response
Description	The mutants with more-pronounced lysis, and then, competition assays with serial plating confirmed their survival was significantly reduced by the TseL-active T6SS-dependent killer
Reference	[1] Kamal F, Liang X, Manera K, et al. Differential Cellular Response to Translocated Toxic Effectors and Physical Penetration by the Type VI Secretion System. Cell Rep. 2020 Jun 16;31(11):107766. doi: 10.1016/j.celrep.2020.107766. PMID: 32553162 [2] Hersch SJ, Manera K, Dong TG. Defending against the Type Six Secretion System: beyond Immunity Genes. Cell Rep. 2020 Oct 13;33(2):108259. doi: 10.1016/j.celrep.2020.108259. PMID: 33053336

Experimental investigation has been performed.

External database links

Locus tag (Gene)	BW25113_RS07820 (gadC)
Coordinates (Strand)	1563211..1564746 (-)
NCBI ID	446168164
RefSeq	NZ_CP009273
Uniprot ID	K4XLQ1_ECOLX, L3BK28_ECOLX, S1IB81_ECOLX, U9XWD2_ECOLX, A0A080J1W4_ECOLX, A0A0A0FFF8_ECOLX, E3PLZ2_ECOH1, A7ZLU0_ECO24, A0A0E1T0E3_ECOLX, B6IAP5_ECOSE, A0A140NAC9_ECOBD, J7RGA5_ECOLX, F4NG05_ECOLX, E0J6C9_ECOLW, B7L7J1_ECO55, A0A1Q8MD55_SHIBO, GADC_SHIFL, A0A0I2C196_SHISO, A0A1X3JHP3_ECOLX, A0A1X3L2L7_ECOLX, F4VF91_ECOLX, F4SIT2_ECOLX, A0A1X3LJA3_ECOLX, A0A1X3J2C6_ECOLX, E6BQR3_ECOLX, E1IT63_ECOLX, E1JCR0_ECOLX, D3QT39_ECOCB, A0A0E1M1Q4_ECOLX, D7XVW2_ECOLX, D7Y0C2_ECOM1, E9T8U4_ECOLX, I4SHH8_ECOLX, GADC_ECOLI, I2SST3_ECOLX, I2UGB7_ECOLX, I2WPI0_ECOLX
KEGG ID	eco:b1492, sfx:S1868, elh:ETEC_1562, ecw:EcE24377A_1681, eck:EC55989_1624, ebd:ECBD_2147, elw:ECW_m1620, ecj:JW1487, ecy:ECSE_1582, eok:G2583_1855, sfl:SF1735
PDB ID	4DJI, 4DJK

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
AA_permease_2	PF13520.8	1.4e-52	13..451

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-12	MATSVQTGKAKQ
Transmembrane helix	13-30	LTLLGFFAITASMVMAVY
Outside	31-39	EYPTFATSG
Transmembrane helix	40-62	FSLVFFLLLGGILWFIPVGLCAA
Inside	63-94	EMATVDGWEEGGVFAWVSNTLGPRWGFAAISF
Transmembrane helix	95-117	GYLQIAIGFIPMLYFVLGALSYI
Outside	118-131	LKWPALNEDPITKT
Transmembrane helix	132-151	IAALIILWALALTQFGGTKY
Inside	152-157	TARIAK
Transmembrane helix	158-180	VGFFAGILLPAFILIALAAIYLH
Outside	181-194	SGAPVAIEMDSKTF
Transmembrane helix	195-217	FPDFSKVGTLVVFVAFILSYMGV
Inside	218-237	EASATHVNEMSNPGRDYPLA
Transmembrane helix	238-260	MLLLMVAAICLSSVGGLSIAMVI
Outside	261-287	PGNEINLSAGVMQTFTVLMSHVAPEIE
Transmembrane helix	288-310	WTVRVISALLLLGVLAEIASWIV
Inside	311-334	GPSRGMYVTAQKNLLPAAFAKMNK
Transmembrane helix	335-357	NGVPVTLVISQLVITSIALIILT
Outside	358-371	NTGGGNNMSFLIAL
Transmembrane helix	372-394	ALTVVIYLCAYFMLFIGYIVLVL
Inside	395-406	KHPDLKRTFNIP
Transmembrane helix	407-429	GGKGVKLVVAIVGLLTSIMAFIV
Outside	430-443	SFLPPDNIQGDSTD
Transmembrane helix	444-466	MYVELLVVSFLVVLALPFILYAV
Inside	467-511	HDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMNDKKH

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9886	-	-

Protein sequence: 512 a.a. .

                                    >RSF000011 NZ_CP009273:c1564746-1563211 [Escherichia coli BW25113]
MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVFFLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVS
NTLGPRWGFAAISFGYLQIAIGFIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVEASATHVNEMSNPGRDYPLAMLL
LMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFTVLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTA
QKNLLPAAFAKMNKNGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVLVLKHPDLK
RTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLVVSFLVVLALPFILYAVHDRKGKANTGVTLE
PINSQNAPKGHFFLHPRARSPHYIVMNDKKH*

                                

Nucleotide sequence: 1536 bp .

                                    >RSF000011 NZ_CP009273:c1564746-1563211 [Escherichia coli BW25113]
ATGGCTACATCAGTACAGACAGGTAAAGCTAAGCAGCTCACATTACTTGGATTCTTTGCCATAACGGCATCGATGGTAAT
GGCTGTTTATGAATACCCTACCTTCGCAACATCGGGCTTTTCATTAGTCTTCTTCCTGCTATTAGGCGGGATTTTATGGT
TTATTCCCGTGGGACTTTGTGCTGCGGAAATGGCCACCGTCGACGGCTGGGAAGAAGGTGGTGTCTTCGCCTGGGTATCA
AATACTCTGGGGCCGAGATGGGGATTTGCAGCGATCTCATTTGGCTATCTGCAAATCGCCATTGGTTTTATTCCGATGCT
CTATTTCGTGTTAGGGGCACTCTCCTACATCCTGAAATGGCCAGCGCTGAATGAAGACCCCATTACCAAAACTATTGCAG
CACTCATCATTCTTTGGGCGCTGGCATTAACGCAGTTTGGTGGCACGAAATACACGGCGCGAATTGCTAAAGTTGGCTTC
TTCGCCGGTATCCTGTTACCTGCATTTATTTTGATCGCATTAGCGGCTATTTATCTGCACTCCGGTGCCCCCGTTGCTAT
CGAAATGGATTCGAAGACCTTCTTCCCTGACTTCTCTAAAGTGGGCACCCTGGTAGTATTTGTTGCCTTCATTTTGAGTT
ATATGGGCGTAGAAGCATCCGCAACCCACGTCAATGAAATGAGCAACCCAGGGCGCGACTATCCGTTGGCTATGTTACTG
CTGATGGTGGCGGCAATCTGCTTAAGCTCTGTTGGTGGTTTGTCTATTGCGATGGTCATTCCGGGTAATGAAATCAACCT
CTCCGCAGGGGTAATGCAAACCTTTACCGTTCTGATGTCCCATGTGGCACCAGAAATTGAGTGGACGGTTCGCGTGATCT
CCGCACTGCTGTTGCTGGGTGTTCTGGCGGAAATCGCCTCCTGGATTGTTGGTCCTTCTCGCGGGATGTATGTAACAGCG
CAGAAAAACCTGCTGCCAGCGGCATTCGCTAAAATGAACAAAAATGGCGTACCGGTAACGCTGGTCATTTCGCAGCTGGT
GATTACGTCTATCGCGTTGATCATCCTCACCAATACCGGTGGCGGTAACAACATGTCCTTCCTGATCGCACTGGCGCTGA
CGGTGGTGATTTATCTGTGTGCTTATTTCATGCTGTTTATTGGCTACATTGTGTTGGTTCTTAAACATCCTGACTTAAAA
CGCACATTTAATATCCCTGGTGGTAAAGGGGTGAAACTGGTCGTGGCAATTGTCGGTCTGCTGACTTCAATTATGGCGTT
TATTGTTTCCTTCCTGCCGCCGGATAACATCCAGGGTGATTCTACCGATATGTATGTTGAATTACTGGTTGTTAGTTTCC
TGGTGGTACTTGCCCTGCCCTTTATTCTCTATGCTGTTCATGATCGTAAAGGCAAAGCAAATACCGGCGTCACTCTGGAG
CCAATCAACAGTCAGAACGCACCAAAAGGTCACTTCTTCCTGCACCCGCGTGCACGTTCACCACACTATATTGTGATGAA
TGACAAGAAACACTAA

                                