SecReT6, T6SS database

Detailed information of Recipient susceptibility factor

Summary

ID	RSF000032
Strain	Escherichia coli BW25113 strain K-12
Replicon	chromosome
Sequence	Protein sequence (162 a.a.); Nucleotide sequence (486 bp)
Response to T6SS attack	protective
Known or putative function	Maintaining envelope integrity
Description	Overexpression of Spy protected the ΔdegP strain and other sensitive E. coli mutants against TseH
Reference	[1] Hersch SJ, Watanabe N, Stietz MS, et al. Envelope stress responses defend against type six secretion system attacks independently of immunity proteins. Nat Microbiol. 2020 May;5(5):706-714. doi: 10.1038/s41564-020-0672-6. Epub 2020 Feb 24. PMID: 32094588 [2] Kamal F, Liang X, Manera K, et al. Differential Cellular Response to Translocated Toxic Effectors and Physical Penetration by the Type VI Secretion System. Cell Rep. 2020 Jun 16;31(11):107766. doi: 10.1016/j.celrep.2020.107766. PMID: 32553162 [3] Hersch SJ, Manera K, Dong TG. Defending against the Type Six Secretion System: beyond Immunity Genes. Cell Rep. 2020 Oct 13;33(2):108259. doi: 10.1016/j.celrep.2020.108259. PMID: 33053336

Experimental investigation has been performed.

External database links

Locus tag (Gene)	BW25113_RS09120 (Spy)
Coordinates (Strand)	1819397..1819882 (-)
NCBI ID	447151728
RefSeq	NZ_CP009273
Uniprot ID	M9H5T4_ECOLX, S1EX41_ECOLX, E3PNC7_ECOH1, A0A0E1SY60_ECOLX, W8SYE2_ECOLX, A0A1X3JGY7_ECOLX, F4SKG1_ECOLX, A0A0E1M2L7_ECOLX, SPY_ECOLI
KEGG ID	ecj:JW1732, eco:b1743, elh:ETEC_1775
PDB ID	6OWX, 6OWZ, 6OWY, 5WNW, 5WO2, 5WO3, 5WO1, 6BIE

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
LTXXQ	PF07813.14	6.8e-24	46..141

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-161	MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKFGPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKVKAEAQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATAE

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
SP(Sec/SPI)	0.9985	pos: 23-24. AHA-AD.	MRKLTALFVASTLALGAANLAHA

Protein sequence: 162 a.a. .

                                    >RSF000032 NZ_CP009273:c1819882-1819397 [Escherichia coli BW25113]
MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKFGPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPP
LEERRAMHDIIASDTFDKVKAEAQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
E*

                                

Nucleotide sequence: 486 bp .

                                    >RSF000032 NZ_CP009273:c1819882-1819397 [Escherichia coli BW25113]
ATGCGTAAATTAACTGCACTGTTTGTTGCCTCTACCCTGGCTCTTGGCGCGGCTAACCTGGCCCATGCCGCAGACACCAC
TACCGCAGCACCGGCTGACGCGAAGCCGATGATGCACCACAAAGGCAAGTTCGGTCCGCATCAGGACATGATGTTCAAAG
ACCTGAACCTGACCGACGCGCAGAAACAGCAGATCCGCGAAATCATGAAAGGCCAGCGTGACCAGATGAAACGTCCGCCG
CTGGAAGAACGCCGCGCAATGCATGACATCATTGCCAGCGATACCTTCGATAAAGTAAAAGCTGAAGCGCAGATCGCAAA
AATGGAAGAACAGCGCAAAGCTAACATGCTGGCGCACATGGAAACCCAGAACAAAATTTACAACATCCTGACGCCGGAAC
AGAAAAAGCAATTTAATGCTAATTTTGAGAAGCGTCTGACAGAACGTCCAGCGGCAAAAGGTAAAATGCCTGCAACTGCT
GAATAA

                                