SecReT6, T6SS database

Detailed information of regulator

Summary

Regulator ID	REG0339
Regulator type	protein
Regulatory pathway (from literatures)	TCS-HK
KEGG pathway (determined by BlastKOALA)	Two-component system (map02020); Cationic antimicrobial peptide (CAMP) resistance (map01503)
Related T6SS	T6SS01214 (Type Unknown)
Strain	Klebsiella pneumoniae strain Kp52.145
Replicon	chromosome
Sequence	Protein sequence (224 a.a.); Nucleotide sequence (672 bp)
Description	Antimicrobial peptides activate the PhoPQ two-component system which, in turn, positively regulates Kp52145 T6SS resulting in increased T6SS-dependent antimicrobial activity.
Reference	[1] Storey D, McNally A, Åstrand M, et al. Klebsiella pneumoniae type VI secretion system-mediated microbial competition is PhoPQ controlled and reactive oxygen species dependent. PLoS Pathog. 2020 Mar 19;16(3):e1007969. doi: 10.1371/journal.ppat.1007969. eCollection 2020 Mar. PMID: 32191774

External database links

Locus tag (Gene)	BN49_RS12735 (phoP)
Coordinates (Strand)	2325637..2326308 (-)
NCBI ID	490252783
RefSeq	NZ_FO834906
Uniprot ID	A0A0H3GRL4_KLEPH, A0A1F2MF15_9ENTR, A0A223UD73_9ENTR, A0A087FMV9_KLEVA, A6T7J8_KLEP7, B5XSN8_KLEP3, A0A1Y0PZG2_KLEPN, R4YCR9_KLEPR
KEGG ID	kpk:A593_20140, kpv:KPNIH29_10080, kvd:KR75_17815, kpn:KPN_01136, kpw:KPNIH30_10555, kvq:SP68_24225, kpe:KPK_3415, kpr:KPR_2181, kpg:KPNIH32_10395, kpm:KPHS_20130, kpy:KPNIH31_09985
KEGG pathway (determined by BlastKOALA)	map02020; map01503
PDB ID	-

Target

Target	Regulation (↓/↑)	Target sequence
BN49_2224 (phoQ)	↓	490249900

Pfam domain hit(s) of regulator

Domain	Pfam ID	E-value	Aligned region
Response_reg	PF00072.26	1e-24	3..112
Trans_reg_C	PF00486.30	8.4e-20	145..220

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-223	MRVLVVEDNALLRHHLKVQLQELGHQVDAAEDAREADYYLGEHLPDIAIVDLGLPDEDGLSLIRRWRSHDVSLPVLVLTAREGWQDKVEVLSAGADDYVTKPFHIEEVAARMQALLRRNSGLASQVISLPPFQVDLSRRELSVNDQPIKLTAFEYTIMETLIRNRGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAEYPQDVITTVRGQGYLFELR

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9970	-	-

Protein sequence of regulator: 224 a.a. .

                                    >REG0339 NZ_FO834906:c2326308-2325637 [Klebsiella pneumoniae]
MRVLVVEDNALLRHHLKVQLQELGHQVDAAEDAREADYYLGEHLPDIAIVDLGLPDEDGLSLIRRWRSHDVSLPVLVLTA
REGWQDKVEVLSAGADDYVTKPFHIEEVAARMQALLRRNSGLASQVISLPPFQVDLSRRELSVNDQPIKLTAFEYTIMET
LIRNRGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAEYPQDVITTVRGQGYLFELR*

                                 

Nucleotide sequence of regulator: 672 bp .

                                    >REG0339 NZ_FO834906:c2326308-2325637 [Klebsiella pneumoniae]
ATGCGCGTACTCGTGGTTGAGGATAATGCCCTGCTGCGTCACCACCTCAAAGTTCAGCTGCAGGAGCTGGGCCATCAGGT
CGATGCGGCGGAAGATGCCAGGGAAGCGGACTACTATCTGGGCGAACATCTCCCGGATATCGCCATCGTCGATCTCGGCC
TGCCGGATGAAGACGGTTTATCACTGATCCGCCGCTGGCGCAGCCACGACGTGTCGCTGCCGGTGCTGGTGCTGACCGCC
CGCGAAGGATGGCAGGATAAAGTGGAAGTGCTGAGCGCCGGGGCGGATGATTACGTCACCAAGCCTTTCCATATTGAAGA
GGTTGCCGCCCGCATGCAGGCGCTGCTGCGCCGTAACAGCGGTCTGGCCTCGCAGGTGATCTCCCTGCCGCCGTTCCAGG
TCGACCTCTCCCGGCGCGAGCTGTCGGTGAATGACCAGCCGATCAAGCTGACCGCCTTTGAATACACCATTATGGAAACC
CTGATCCGTAACCGCGGCAAAGTGGTCAGCAAAGATTCGCTGATGCTCCAGCTTTACCCGGATGCCGAACTGCGAGAAAG
CCACACCATCGACGTGCTGATGGGTCGGCTGCGCAAGAAAATTCAGGCTGAATACCCACAGGACGTCATCACCACGGTGC
GCGGCCAGGGCTATCTGTTCGAATTGCGCTGA

                                