Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negative regulation.
Experimental investigation has been performed.
Bioinformatics investigation has been performed

Regulator ID	Locus tag (Gene)	Related T6SS	Regulator type	Regulatory pathway	Organism	Replicon (Accession)	Coordinates (Strand)	NCBI ID	Target (Regulation)
REG0003	RT90_01105 (rcsC)	T6SS01264	protein	TCS-HK	Serratia marcescens strain RM66262	chromosome (JWLO01000001)	232638..235499 (+)	735006326	RT90_01115 (rcsB) (↑)
REG0045	PA0610 (prtN)	T6SS00003	protein	TCS-HK	Pseudomonas aeruginosa PAO1	chromosome (NC_002516)	672777..673091 (-)	15595807	PA0611 (prtR) (↑)
REG0084	chvG	T6SS00016	protein	TCS-HK	Agrobacterium fabrum str. C58	chromosome (NC_003062)	34679..36469 (-)		chvI (↓)
REG0100	VP_RS12495 (barA)	T6SS00043 ; T6SS00044	protein	TCS-HK	Vibrio parahaemolyticus RIMD 2210633	chromosome (NC_004603)	2712492..2715290 (+)	491625032	Hcp expression and secretion (↓)
REG0128	BMAA0745 (VirA)	T6SS00070	protein	TCS-HK	Burkholderia mallei ATCC 23344	chromosome (NC_006349)	762932..764776 (+)	490305845	Hcp1 (BMAA0742) expression (↓)
REG0130	BMAA0745 (VirA)	T6SS00070	protein	TCS-HK	Burkholderia mallei ATCC 23344	chromosome (NC_006349)	763148..764776 (+)	53717365	BMAA0746 (VirG) (↓)
REG0138	BPSS1494 (virG)	T6SS00077	protein	TCS-HK; BsaN regulon	Burkholderia pseudomallei K96243	chromosome (NC_006351)	2037977..2038714 (-)	490663798	BPSS1495 (virA) (↓)
REG0145	BPSS1495 (virA)	T6SS00077	protein	TCS-HK	Burkholderia pseudomallei K96243	chromosome (NC_006351)	2038711..2040555 (-)	53722517	hcp1 expression (↓)
REG0150	Psyr_3698 (gacS)	T6SS00086	protein	TCS-HK	Pseudomonas syringae pv. syringae B728a	chromosome (NC_007005)	4385550..4388303 (+)	499587812	T6SS transcription (↓)
REG0175	FTN_RS08805 (KdpD)	T6SS00158	protein	TCS-HK	Francisella tularensis subsp. novicida U112	chromosome (NC_008601)	1838601..1841282 (-)	489131611	FTN_1465 (pmrA) (↓)
REG0244	ETAE_2059 (phoP)	T6SS00372	protein	TCS-HK	Edwardsiella tarda EIB202	chromosome (NC_013508)	2172565..2173239 (+)	502611910	ETAE_0886 (EsrB) (↓)
REG0318	Y113_RS04835 (esrC)	T6SS00791	protein	TCS-HK	Edwardsiella tarda strain PPD130/91	chromosome (NZ_JACQ01000008)	20632..23367 (-)	1624663318	EvpQ protein level (↓)
REG0323	GT391_RS16925 (phoP)	T6SS01170	protein	TCS-HK	Pectobacterium brasiliense strain 1692	chromosome (NZ_CP047495)	3795787..3796476 (-)	497961604	GT391_RS06470 (expR1) (↑)
REG0331	Pchl3084_3491 (gacA)	T6SS01266 ; T6SS01267	protein	TCS-HK	Pseudomonas chlororaphis subsp. aureofaciens 30-84	chromosome (NZ_CM001559)	4134794..4135435 (-)	496335231	Pchl3084_5419 (rsmY)promotor (↓); Pchl3084_1190 (rsmZ)promotor (↓); Pchl3084_1189 (rpoS)transcript (↓)
REG0339	BN49_RS12735 (phoP)	T6SS01214	protein	TCS-HK	Klebsiella pneumoniae strain Kp52.145	chromosome (NZ_FO834906)	2325637..2326308 (-)	490252783	BN49_2224 (phoQ) (↓)
REG0357	PPECC33_RS11180 (BaeS)	T6SS01034	protein	TCS-HK	Escherichia coli PCN033	chromosome (NZ_CP006632)	2271464..2272867 (+)	446597798	PPECC33_RS11185 (BaeR) (↓)