SecReT6, T6SS database

Detailed information of regulator

Summary

Regulator ID	REG0130
Regulator type	protein
Regulatory pathway (from literatures)	TCS-HK
KEGG pathway (determined by BlastKOALA)	-
Related T6SS	T6SS00070 (Type i1)
Strain	Burkholderia mallei ATCC 23344
Replicon	chromosome
Sequence	Protein sequence (543 a.a.); Nucleotide sequence (1629 bp)
Description	Acid pH upregulates TssM and Hcp1 expression through VirAG.
Reference	[1] Chua J, Senft JL, Lockett SJ, et al. pH Alkalinization by Chloroquine Suppresses Pathogenic Burkholderia Type 6 Secretion System 1 and Multinucleated Giant Cells. Infect Immun. 2016 Dec 29;85(1). pii: e00586-16. doi: 10.1128/IAI.00586-16. Print 2017 Jan. PMID: 27799332 [2] Schell MA, Ulrich RL, Ribot WJ, et al. Type VI secretion is a major virulence determinant in Burkholderia mallei. Mol Microbiol. 2007 Jun;64(6):1466-85. PMID: 17555434 [3] Burtnick MN, Brett PJ. Burkholderia mallei and Burkholderia pseudomallei cluster 1 type VI secretion system gene expression is negatively regulated by iron and zinc. PLoS One. 2013 Oct 11;8(10):e76767. doi: 10.1371/journal.pone.0076767. eCollection 2013. PMID: 24146925

External database links

Locus tag (Gene)	BMAA0745 (VirA)
Coordinates (Strand)	763148..764776 (+)
NCBI ID	53717365
RefSeq	NC_006349
Uniprot ID	A0A0H2WE15_BURMA
KEGG ID	bma:BMAA0739
KEGG pathway (determined by BlastKOALA)	-
PDB ID	-

Target

Target	Regulation (↓/↑)	Target sequence
BMAA0746 (VirG)	↓	53717366

Pfam domain hit(s) of regulator

Domain	Pfam ID	E-value	Aligned region
HATPase_c	PF02518.28	5.5e-18	382..520
MASE1	PF05231.16	3.2e-12	1..251

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-20	VWPAPVLGIALLLHCRRPLA
Transmembrane helix	21-43	WWLGAGSLFATLLAAGSLDWVPW
Outside	44-46	HVD
Transmembrane helix	47-69	ALFAAVNVVEAIAGAWLARRYLI
Inside	70-81	AGGGVDTLRGFA
Transmembrane helix	82-104	MFVLLLPLGMAAMHATLAAAILS
Outside	105-118	VWMNDREWLSEWSR
Transmembrane helix	119-141	AYAANALALLVLLPALLTWNARA
Inside	142-152	CGAAWSRSRNQ
Transmembrane helix	153-175	LPVLATFGSLCFASIWGYQAEVA
Outside	176-189	RALLALSLSWAALE
Transmembrane helix	190-212	GGLVLASQLNALSAIALIAMTLG
Inside	213-224	GRGPYAWHHDGH
Transmembrane helix	225-247	GVWALQVDLIGVAILSMCIAIAT
Outside	248-542	GERRRLARQIERSRRFESLGFFAGGIVHDFSNVLTTIDCHAEYASERLASGARADDSIDEIHRAVERGRDLAAQILLAARRGEPVLTRVSVDEVVDEALAATRAAAWRGVEIVRCGDGARDGARRGDVRHVVFADRSQLARAVMNLVGNAVRAARATVVVRVGVNGGVNGGASDGASRGANDGGAPAARAFDVAIGEAAMPGGVWIEVSDDGEGIDVEPLDRIFEPFYSNRSGAPGTGLGLAIVAGAALAHRGQIAVATDRGAGSCFRLTLPAAWGTGAPGAMDAGAPGAVQERT

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9334	-	-

Protein sequence of regulator: 543 a.a. .

                                    >REG0130 NC_006349:763148-764776 [Burkholderia mallei ATCC 23344]
VWPAPVLGIALLLHCRRPLAWWLGAGSLFATLLAAGSLDWVPWHVDALFAAVNVVEAIAGAWLARRYLIAGGGVDTLRGF
AMFVLLLPLGMAAMHATLAAAILSVWMNDREWLSEWSRAYAANALALLVLLPALLTWNARACGAAWSRSRNQLPVLATFG
SLCFASIWGYQAEVARALLALSLSWAALEGGLVLASQLNALSAIALIAMTLGGRGPYAWHHDGHGVWALQVDLIGVAILS
MCIAIATGERRRLARQIERSRRFESLGFFAGGIVHDFSNVLTTIDCHAEYASERLASGARADDSIDEIHRAVERGRDLAA
QILLAARRGEPVLTRVSVDEVVDEALAATRAAAWRGVEIVRCGDGARDGARRGDVRHVVFADRSQLARAVMNLVGNAVRA
ARATVVVRVGVNGGVNGGASDGASRGANDGGAPAARAFDVAIGEAAMPGGVWIEVSDDGEGIDVEPLDRIFEPFYSNRSG
APGTGLGLAIVAGAALAHRGQIAVATDRGAGSCFRLTLPAAWGTGAPGAMDAGAPGAVQERT*

                                 

Nucleotide sequence of regulator: 1629 bp .

                                    >REG0130 NC_006349:763148-764776 [Burkholderia mallei ATCC 23344]
GTGTGGCCCGCGCCGGTGCTCGGCATCGCGCTGCTGCTGCACTGCCGGCGGCCGCTCGCATGGTGGCTCGGCGCGGGTTC
GCTGTTCGCCACGCTGCTCGCCGCGGGCTCGCTCGACTGGGTGCCGTGGCACGTCGACGCGCTGTTCGCGGCCGTCAACG
TCGTCGAGGCGATCGCGGGCGCGTGGCTCGCGCGGCGCTATCTGATCGCGGGCGGCGGGGTGGACACGCTGCGCGGCTTC
GCGATGTTCGTGCTGCTGCTGCCGCTCGGCATGGCGGCGATGCACGCGACGCTCGCGGCGGCGATCCTGTCGGTATGGAT
GAACGACCGCGAATGGCTGAGCGAATGGTCGCGCGCGTATGCGGCGAACGCGCTCGCGCTGCTCGTGCTGCTGCCGGCGC
TCCTCACGTGGAACGCGCGCGCATGCGGCGCGGCGTGGTCGCGCAGCCGCAATCAGTTGCCGGTGCTCGCGACGTTCGGC
TCGCTGTGCTTCGCGTCGATATGGGGATACCAGGCCGAGGTCGCGCGCGCGCTGCTCGCGCTGTCGCTGAGCTGGGCCGC
GCTCGAGGGCGGCCTCGTGCTCGCGTCCCAGCTCAACGCGCTCTCGGCGATCGCCCTGATCGCGATGACGCTCGGCGGGC
GCGGCCCGTATGCGTGGCATCACGACGGCCACGGCGTCTGGGCGCTGCAGGTCGATCTGATCGGCGTCGCGATCCTGTCG
ATGTGCATCGCGATCGCGACGGGCGAGCGGCGGCGGCTTGCGCGGCAGATCGAACGATCGCGCCGTTTCGAGTCGCTCGG
CTTTTTCGCCGGCGGAATCGTGCACGATTTCAGCAACGTGCTGACGACGATCGATTGCCACGCCGAATACGCGTCCGAGC
GGCTCGCGAGCGGCGCGCGCGCGGACGATTCGATCGACGAGATTCATCGCGCGGTCGAACGCGGCCGCGATCTGGCGGCG
CAGATCCTGCTCGCCGCGCGCCGCGGCGAGCCCGTGCTGACGCGGGTGAGCGTCGACGAGGTCGTCGACGAGGCGCTCGC
CGCGACACGCGCGGCCGCGTGGCGCGGCGTCGAGATCGTTCGATGCGGCGATGGCGCGCGCGACGGCGCCCGGCGTGGCG
ACGTTCGTCACGTCGTATTCGCCGATCGCAGCCAGCTCGCGCGCGCGGTCATGAATCTCGTCGGCAATGCGGTGCGCGCG
GCGCGCGCGACGGTTGTCGTGCGTGTCGGGGTGAACGGCGGGGTGAATGGCGGCGCGAGCGATGGGGCGAGCCGCGGCGC
GAACGACGGCGGCGCGCCGGCCGCTCGCGCATTCGACGTCGCGATCGGCGAAGCGGCGATGCCGGGCGGCGTGTGGATCG
AGGTGAGCGACGACGGCGAAGGCATCGACGTCGAGCCGCTCGACCGCATCTTCGAGCCGTTCTATTCGAACCGCTCCGGC
GCGCCGGGGACGGGGCTGGGTCTCGCGATCGTCGCGGGCGCGGCGCTCGCGCACCGCGGGCAGATCGCCGTCGCGACGGA
TCGGGGCGCGGGCAGTTGCTTCCGGCTCACGTTGCCGGCGGCATGGGGAACGGGCGCGCCCGGCGCAATGGACGCCGGCG
CGCCCGGCGCAGTACAGGAGAGGACATGA

                                