Detailed information of T6SS
Note: only experimentally validated proteins are displayed in the graph.
Summary
| T6SS ID | T6SS00070  |
| T6SS type | Type i1 |
| Strain | Burkholderia mallei ATCC 23344 |
| Replicon (RefSeq) | chromosome II (NC_006349) |
| Location | 735641..772695 |
| Function | anti-eukaryotic; effector translocation; virulence |
| Comment | Virulence in Syrian hamster model; intracellular growth, actin polymerisation and mononuclear giant cell formation in cultured macrophages. |
| References | [1] Shanks J et al (2009) Burkholderia mallei tssM encodes a putative deubiquitinase that is secreted and expressed inside infected RAW 264.7 murine macrophages. Infect Immun. 77(4):1636-48. [PudMed:19168747] [2] Schell MA et al (2007) Type VI secretion is a major virulence determinant in Burkholderia mallei. Mol Microbiol. 64(6):1466-85. [PudMed:17555434] [3] Fisher NA et al (2012) The Madagascar hissing cockroach as a novel surrogate host for Burkholderia pseudomallei, B. mallei and B. thailandensis. BMC Microbiol. 0.58125. [PudMed:22892068] [4] Burtnick MN et al (2010) Burkholderia mallei cluster 1 type VI secretion mutants exhibit growth and actin polymerization defects in RAW 264.7 murine macrophages. Infect Immun. 78(1):88-99. [PudMed:19884331] |
Experimental investigation has been performed.T6SS components and genome coordinates
| Component | TssA | TssB | TssC | TssD | TssE | TssF | TssG | TssH | TssI | TssJ | TssK | TssL | TssM | VirA | VirG |
| Number | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| Component ID | Component | Locus tag (Gene) | Coordinate | Strand | Size (bp) | NCBI ID | Product |
|---|---|---|---|---|---|---|---|
| BMA_RS19500 | 732317..732814 | - | 498 | WP_004201904.1 | hypothetical protein | ||
| BMA_RS19505 | 732932..733816 | - | 885 | WP_004266679.1 | metal-dependent hydrolase | ||
| BMA_RS19510 | 733927..735047 | - | 1121 | WP_038802950.1 | IS3 family transposase | ||
| BMA_RS19515 | 735241..735548 | - | 308 | pseudogene | DDE-type integrase/transposase/recombinase | ||
| BMA_RS19520 | 735641..736273 | - | 633 | WP_004528803.1 | GTP cyclohydrolase I | ||
| BMA_RS19525 | 736278..736571 | - | 294 | WP_004551882.1 | type VI secretion system protein TssN | ||
| BMA_RS19530 | 736530..736775 | + | 246 | WP_004201911.1 | hypothetical protein | ||
| BMA_RS19535 | 736735..737958 | - | 1224 | WP_162473488.1 | Ig-like domain-containing protein | ||
| T6CP008459 | TssM | BMA_RS19540 (tssM-5) | 738692..742738 | - | 4047 | WP_174977041.1 | type VI secretion system membrane subunit TssM-5 |
| T6CP000596 | TssL | BMA_RS19545 | 742735..743400 | - | 666 | WP_004184888.1 | type VI secretion system protein TssL |
| T6CP000597 | TssK | BMA_RS19550 (tssK) | 743397..744794 | - | 1398 | WP_004206515.1 | type VI secretion system baseplate subunit TssK |
| T6CP009350 | TssJ | BMA_RS19555 (tssJ) | 744827..745624 | - | 798 | WP_004200965.1 | type VI secretion system lipoprotein TssJ |
| BMA_RS19560 | 745634..746026 | - | 393 | WP_004200966.1 | type VI secretion system protein TssI | ||
| BMA_RS19565 | 746055..746816 | - | 762 | WP_004200968.1 | type VI secretion system protein TssH | ||
| BMA_RS19570 | 746813..747877 | - | 1065 | WP_004206517.1 | type VI secretion system protein TssG | ||
| BMA_RS19575 | 747895..750537 | - | 2643 | WP_004206518.1 | type VI secretion system protein TssF | ||
| T6CP000598 | TssI | BMA_RS19580 (tssI) | 750563..753586 | - | 3024 | WP_004184913.1 | type VI secretion system tip protein VgrG |
| T6CP000599 | TssH | BMA_RS19585 | 753613..756696 | - | 3084 | WP_004200969.1 | ATP-dependent protease ClpV |
| T6CP000600 | TssG | BMA_RS19590 (tssG) | 756683..757705 | - | 1023 | WP_004200970.1 | type VI secretion system baseplate subunit TssG |
| T6CP000601 | TssF | BMA_RS19595 (tssF) | 757693..759435 | - | 1743 | WP_004200971.1 | type VI secretion system baseplate subunit TssF |
| T6CP008460 | TssE | BMA_RS19600 (tssE) | 759472..759933 | - | 462 | WP_004184744.1 | type VI secretion system baseplate subunit TssE |
| T6CP008461 | TssD | BMA_RS19605 (hcp1) | 759926..760435 | - | 510 | WP_004200972.1 | type VI secretion system effector Hcp1 |
| T6CP000602 | TssC | BMA_RS19610 (tssC) | 760655..762181 | - | 1527 | WP_004200974.1 | type VI secretion system contractile sheath large subunit |
| T6CP000603 | TssB | BMA_RS19615 (tssB) | 762178..762672 | - | 495 | WP_004200975.1 | type VI secretion system contractile sheath small subunit |
| T6CP012439 | VirA | BMA_RS19620 | 762932..764776 | + | 1845 | WP_004200976.1 | sensor histidine kinase VirA |
| T6CP012440 | VirG | BMA_RS19625 | 764773..765510 | + | 738 | WP_004206312.1 | response regulator VirG |
| T6CP008462 | TssA | BMA_RS19630 (tssA) | 765501..767435 | + | 1935 | WP_004200978.1 | type VI secretion system protein TssA |
| BMA_RS19635 | 767778..768155 | - | 378 | WP_004184741.1 | hypothetical protein | ||
| BMA_RS19640 | 768235..769356 | - | 1122 | WP_004266405.1 | trimeric autotransporter adhesin BimA | ||
| BMA_RS19645 | 769385..770746 | - | 1362 | WP_004200982.1 | autotransporter strand-loop-strand O-heptosyltransferase | ||
| BMA_RS19650 | 770893..771918 | - | 1026 | WP_004206324.1 | N-acetylmuramoyl-L-alanine amidase | ||
| BMA_RS19655 | 772063..772452 | - | 390 | WP_004184656.1 | hypothetical protein | ||
| BMA_RS19660 | 772957..773571 | - | 615 | WP_004200984.1 | SCO family protein | ||
| BMA_RS19665 | 773568..774404 | - | 837 | WP_004200985.1 | formylglycine-generating enzyme family protein | ||
| BMA_RS19670 (nirK) | 774429..775991 | - | 1563 | WP_004200986.1 | nitrite reductase, copper-containing | ||
| BMA_RS19675 | 776399..776839 | - | 441 | WP_004184911.1 | SRPBCC family protein |
Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files
Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
Experimental investigation has been performed.
Bioinformatics investigation has been performed.| Regulator ID | Locus tag (Gene) | Regulator type | Regulatory pathway | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Target (Regulation) |
|---|---|---|---|---|---|---|---|---|
| REG0126 |
BMAA1517 | protein | Transcription regulator | Burkholderia mallei ATCC 23344 | chromosome (NC_006349) | 1646532..1647593 (-) | 499517952 | Hcp1 (BMAA0742) expression (↓) |
| REG0127 |
BMAA0746 (VirG) | protein | TCS-RR | Burkholderia mallei ATCC 23344 | chromosome (NC_006349) | 764773..765510 (+) | 490311617 | Hcp1 (BMAA0742) expression (↓) |
| REG0128 |
BMAA0745 (VirA) | protein | TCS-HK | Burkholderia mallei ATCC 23344 | chromosome (NC_006349) | 762932..764776 (+) | 490305845 | Hcp1 (BMAA0742) expression (↓) |
| REG0129 |
BMAA0746 (VirG) | protein | TCS-RR | Burkholderia mallei ATCC 23344 | chromosome (NC_006349) | 764773..765510 (+) | 53717366 | BMAA0742 (Hcp1) expression (↓); BMAA0729.1 (TssM1) expression (↓) |
| REG0130 |
BMAA0745 (VirA) | protein | TCS-HK | Burkholderia mallei ATCC 23344 | chromosome (NC_006349) | 763148..764776 (+) | 53717365 | BMAA0746 (VirG) (↓) |
Experimental investigation has been performed. Bioinformatics investigation has been performed.| Effector ID | Locus tag (Gene) | Classification | Cognate immunity protein | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
|---|---|---|---|---|---|---|---|
| EFF00086 |
BMA_RS19535 | Tse | - | Burkholderia mallei ATCC 23344 | chromosome (NC_006349) | 736735..737958 (-) | 77358950 |
| EFF00087 |
BMA_RS19580 | - | - | Burkholderia mallei ATCC 23344 | chromosome (NC_006349) | 750563..753586 (-) | 53717363 |
| EFF00088 |
BMA_RS19605 | - | - | Burkholderia mallei ATCC 23344 | chromosome (NC_006349) | 759926..760435 (-) | 77358963 |
Experimental investigation has been performed. Bioinformatics investigation has been performed.| Immunity protein ID | Locus tag (Gene) | Cognate effector | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
|---|
Experimental investigation has been performed.| Accessory protein ID | Locus tag (Gene) | Type | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Related structure protein | Related effector |
|---|