Detailed information of regulator


Summary

Regulator ID REG0128 experimental
Regulator type protein
Regulatory pathway (from literatures) TCS-HK
KEGG pathway (determined by BlastKOALA) -
Related T6SS T6SS00070 experimental (Type i1)
Strain Burkholderia mallei ATCC 23344
Replicon chromosome
Sequence Protein sequence (615 a.a.); Nucleotide sequence (1845 bp)
Description virAG and BMAA1517 independently control transcription of this T6S cluster.VirAG and BMAA1517 sense specific conditions in the host environment, and in response, activate the cluster 1 T6S genes.
Reference
External database links

Locus tag (Gene) BMAA0745 (VirA)
Coordinates (Strand) 762932..764776 (+)
NCBI ID 490305845
RefSeq NC_006349
Uniprot ID A5PM42_BURML, C4B107_BURML, A0A0E1TMJ9_BURPE
KEGG ID bmae:DM78_3362, bmai:DM57_11865, bmaf:DM51_4445
KEGG pathway (determined by BlastKOALA) -
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
Hcp1 (BMAA0742) expression ↓ -
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
HATPase_c PF02518.28 6.9e-18 454..592
MASE1 PF05231.16 2.2e-13 63..323
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-32 MHGNSIDAGPPARARPARITRTARTAPRRDDR
Transmembrane helix 33-52 AFAPGGALNAAGLLLLLFYA
Outside 53-66 NHLLSRPGACTSLN
Transmembrane helix 67-86 HTLSAVVWPAPVLGIALLLH
Inside 87-92 CRRPLA
Transmembrane helix 93-115 WWLGAGSLFATLLAAGSLDWVPW
Outside 116-118 HVD
Transmembrane helix 119-141 ALFAAVNVVEAIAGAWLARRYLI
Inside 142-153 AGGGVDTLRGFA
Transmembrane helix 154-176 MFVLLLPLGMAAMHATLAAAILS
Outside 177-190 VWMNDREWLSEWSR
Transmembrane helix 191-213 AYAANALALLVLLPALLTWNARA
Inside 214-224 CGAAWSRSRNQ
Transmembrane helix 225-247 LPVLATFGSLCFASIWGYQAEVA
Outside 248-261 RALLALSLSWAALE
Transmembrane helix 262-284 GGLVLASQLNALSAIALIAMTLG
Inside 285-296 GRGPYAWHHDGH
Transmembrane helix 297-319 GVWALQVDLIGVAILSMCIAIAT
Outside 320-614 GERRRLARQIERSRRFESLGFFAGGIVHDFSNVLTTIDCHAEYASERLASGARADDSIDEIHRAVERGRDLAAQILLAARRGEPVLTRVSVDEVVDEALAATRAAAWRGVEIVRCGDGARDGARRGDVRHVVFADRSQLARAVMNLVGNAVRAARATVVVRVGVNGGVNGGASDGASRGANDGGAPAARAFDVAIGEAAMPGGVWIEVSDDGEGIDVEPLDRIFEPFYSNRSGAPGTGLGLAIVAGAALAHRGQIAVATDRGAGSCFRLTLPAAWGTGAPGAMDAGAPGAVQERT


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.8692 - -


  Protein sequence of regulator: 615 a.a.    .

>REG0128 NC_006349:762932-764776 [Burkholderia mallei ATCC 23344]
MHGNSIDAGPPARARPARITRTARTAPRRDDRAFAPGGALNAAGLLLLLFYANHLLSRPGACTSLNHTLSAVVWPAPVLG
IALLLHCRRPLAWWLGAGSLFATLLAAGSLDWVPWHVDALFAAVNVVEAIAGAWLARRYLIAGGGVDTLRGFAMFVLLLP
LGMAAMHATLAAAILSVWMNDREWLSEWSRAYAANALALLVLLPALLTWNARACGAAWSRSRNQLPVLATFGSLCFASIW
GYQAEVARALLALSLSWAALEGGLVLASQLNALSAIALIAMTLGGRGPYAWHHDGHGVWALQVDLIGVAILSMCIAIATG
ERRRLARQIERSRRFESLGFFAGGIVHDFSNVLTTIDCHAEYASERLASGARADDSIDEIHRAVERGRDLAAQILLAARR
GEPVLTRVSVDEVVDEALAATRAAAWRGVEIVRCGDGARDGARRGDVRHVVFADRSQLARAVMNLVGNAVRAARATVVVR
VGVNGGVNGGASDGASRGANDGGAPAARAFDVAIGEAAMPGGVWIEVSDDGEGIDVEPLDRIFEPFYSNRSGAPGTGLGL
AIVAGAALAHRGQIAVATDRGAGSCFRLTLPAAWGTGAPGAMDAGAPGAVQERT*

  Nucleotide sequence of regulator: 1845 bp    .

>REG0128 NC_006349:762932-764776 [Burkholderia mallei ATCC 23344]
ATGCACGGAAATTCCATCGATGCCGGGCCGCCCGCGCGCGCCCGGCCCGCACGCATCACGCGAACGGCGCGCACCGCGCC
GCGCCGCGACGATCGCGCGTTCGCGCCGGGCGGCGCGCTGAACGCGGCGGGGCTGCTGCTGCTGCTGTTCTACGCGAATC
ATCTGCTGTCCCGGCCCGGCGCGTGCACGTCGCTCAATCACACGCTGTCGGCCGTCGTGTGGCCCGCGCCGGTGCTCGGC
ATCGCGCTGCTGCTGCACTGCCGGCGGCCGCTCGCATGGTGGCTCGGCGCGGGTTCGCTGTTCGCCACGCTGCTCGCCGC
GGGCTCGCTCGACTGGGTGCCGTGGCACGTCGACGCGCTGTTCGCGGCCGTCAACGTCGTCGAGGCGATCGCGGGCGCGT
GGCTCGCGCGGCGCTATCTGATCGCGGGCGGCGGGGTGGACACGCTGCGCGGCTTCGCGATGTTCGTGCTGCTGCTGCCG
CTCGGCATGGCGGCGATGCACGCGACGCTCGCGGCGGCGATCCTGTCGGTATGGATGAACGACCGCGAATGGCTGAGCGA
ATGGTCGCGCGCGTATGCGGCGAACGCGCTCGCGCTGCTCGTGCTGCTGCCGGCGCTCCTCACGTGGAACGCGCGCGCAT
GCGGCGCGGCGTGGTCGCGCAGCCGCAATCAGTTGCCGGTGCTCGCGACGTTCGGCTCGCTGTGCTTCGCGTCGATATGG
GGATACCAGGCCGAGGTCGCGCGCGCGCTGCTCGCGCTGTCGCTGAGCTGGGCCGCGCTCGAGGGCGGCCTCGTGCTCGC
GTCCCAGCTCAACGCGCTCTCGGCGATCGCCCTGATCGCGATGACGCTCGGCGGGCGCGGCCCGTATGCGTGGCATCACG
ACGGCCACGGCGTCTGGGCGCTGCAGGTCGATCTGATCGGCGTCGCGATCCTGTCGATGTGCATCGCGATCGCGACGGGC
GAGCGGCGGCGGCTTGCGCGGCAGATCGAACGATCGCGCCGTTTCGAGTCGCTCGGCTTTTTCGCCGGCGGAATCGTGCA
CGATTTCAGCAACGTGCTGACGACGATCGATTGCCACGCCGAATACGCGTCCGAGCGGCTCGCGAGCGGCGCGCGCGCGG
ACGATTCGATCGACGAGATTCATCGCGCGGTCGAACGCGGCCGCGATCTGGCGGCGCAGATCCTGCTCGCCGCGCGCCGC
GGCGAGCCCGTGCTGACGCGGGTGAGCGTCGACGAGGTCGTCGACGAGGCGCTCGCCGCGACACGCGCGGCCGCGTGGCG
CGGCGTCGAGATCGTTCGATGCGGCGATGGCGCGCGCGACGGCGCCCGGCGTGGCGACGTTCGTCACGTCGTATTCGCCG
ATCGCAGCCAGCTCGCGCGCGCGGTCATGAATCTCGTCGGCAATGCGGTGCGCGCGGCGCGCGCGACGGTTGTCGTGCGT
GTCGGGGTGAACGGCGGGGTGAATGGCGGCGCGAGCGATGGGGCGAGCCGCGGCGCGAACGACGGCGGCGCGCCGGCCGC
TCGCGCATTCGACGTCGCGATCGGCGAAGCGGCGATGCCGGGCGGCGTGTGGATCGAGGTGAGCGACGACGGCGAAGGCA
TCGACGTCGAGCCGCTCGACCGCATCTTCGAGCCGTTCTATTCGAACCGCTCCGGCGCGCCGGGGACGGGGCTGGGTCTC
GCGATCGTCGCGGGCGCGGCGCTCGCGCACCGCGGGCAGATCGCCGTCGCGACGGATCGGGGCGCGGGCAGTTGCTTCCG
GCTCACGTTGCCGGCGGCATGGGGAACGGGCGCGCCCGGCGCAATGGACGCCGGCGCGCCCGGCGCAGTACAGGAGAGGA
CATGA