Detailed information of component protein


Summary

Component ID T6CP000599
Component protein type TssH
T6SS ID (Type) T6SS00070 experimental (Type i1)
Strain Burkholderia mallei ATCC 23344
Replicon chromosome II
Sequence Protein sequence (1028 a.a.); Nucleotide sequence (3084 bp)
Reference
[1] Schell MA, Ulrich RL, Ribot WJ, et al. Type VI secretion is a major virulence determinant in Burkholderia mallei. Mol Microbiol. 2007 Jun;64(6):1466-85. PMID: 17555434
[2] Shanks J, Burtnick MN, Brett PJ, et al. Burkholderia mallei tssM encodes a putative deubiquitinase that is secreted and expressed inside infected RAW 264.7 murine macrophages. Infect Immun. 2009 Apr;77(4):1636-48. doi: 10.1128/IAI.01339-08. Epub 2009 Jan 21. PMID: 19168747
[3] Burtnick MN, DeShazer D, Nair V, et al. Burkholderia mallei cluster 1 type VI secretion mutants exhibit growth and actin polymerization defects in RAW 264.7 murine macrophages. Infect Immun. 2010 Jan;78(1):88-99. doi: 10.1128/IAI.00985-09. Epub 2009 Nov 2. PMID: 19884331
[4] Fisher NA, Ribot WJ, Applefeld W, et al. The Madagascar hissing cockroach as a novel surrogate host for Burkholderia pseudomallei, B. mallei and B. thailandensis. BMC Microbiol. 2012 Jun 22;12:117. doi: 10.1186/1471-2180-12-117. PMID: 22892068
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BMA_RS19585
Coordinate (Strand) 753613..756696 (-)
NCBI ID WP_004200969.1
RefSeq NC_006349
Uniprot ID A5PM49_BURML, A0A0H2WDH5_BURMA
KEGG ID bmaf:DM51_4438, bma:BMAA0738
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
AAA_2 PF07724.16 7.8e-41 688..855
AAA_lid_9 PF17871.3 4.9e-33 365..456
ClpB_D2-small PF10431.11 5.2e-12 860..929
AAA PF00004.31 5.2e-09 227..336
Clp_N PF02861.22 3.6e-05 28..75
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-1027 MLLVDLKPLIEQLNPYCRNALESAVGACVARRHDDVAVEHLLARLCDEPSADVALLLRACGADAARLRRQADAALDARPAGDGGRPAFAPSLLALLQDAWLIASLELGDTHIRSAAVLAAAVARAARQPSPGGDDVLQSLRKDALVARFASGACARSIESRASGALDAAPGEASDTRDASSAIARYCEDFTAKARAGGIDPVFGRDAEIRQIVDILARRRKNNPVCVGEPGVGKTAVVEGLALRIAEGDVPATLRGATLLGLDLGMLQAGASVKGEFEQRLKRVIAEIRASQTPVVLFIDEAHTLIGAGGAAGASDAANLLKPALARGELRTIAATTWSEYKKYFEKDAALARRFQPVKLDSPDVATSVMILRGLKERYQDAHGVTIRDDALVAAAELSARYITGRQLPDKAIDLLDTACARVKVRQQTKPAALEDAQRAIQALERERRALRDELAERCAPDTPRVADIDRELAALSARAGALRDAWAAQRDAAQALVDARRACRAAADATAATDAAGTTGTAHAGELADSADVSDIADVAKMADAADAAPADAETGSSARDADARMPAGTCARVAPNPDARARPPLRAPHADARERAARALAEATRRFEHAQRDTPLVRIDVDPDAIADVVADWTGIAAGKLRRDRANVMLRLADTLRRRIRGQDHAIEQISEAVKAGAAGVHDPRRPLGVFLLAGPSGTGKTETALAVADALFGDERSIVVVNMSEFQERHDVSRLIGSPPGYVGYGEGGMLTEAVRQRPYSVVLLDEVEKAHPDVLNLFYQVFDKGSLSDGEGKEVDFANTVIFLTSNLGADIIADIAARGARPDPDAMRAAVRPALSRHFKPALLARMTEIPYAPLAPDTLADIARLKLERIAARVAAQHATRIVYDDAVVAHVAARCTEVESGARNVDFILQRHVLPALAQHVLVCAGNAAPMPAIRVAIDAGGRFVVSNDAPADSDARDRSDASDAIDAIDAVDAVDAVDAPNAPNAPNAPNAPNAPNAPNAPNAPNAPNAPNAPNAPNAR


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9883 - -


  Protein sequence: 1028 a.a.    

>T6CP000599 NC_006349:c756696-753613 [Burkholderia mallei ATCC 23344] [TssH]
MLLVDLKPLIEQLNPYCRNALESAVGACVARRHDDVAVEHLLARLCDEPSADVALLLRACGADAARLRRQADAALDARPA
GDGGRPAFAPSLLALLQDAWLIASLELGDTHIRSAAVLAAAVARAARQPSPGGDDVLQSLRKDALVARFASGACARSIES
RASGALDAAPGEASDTRDASSAIARYCEDFTAKARAGGIDPVFGRDAEIRQIVDILARRRKNNPVCVGEPGVGKTAVVEG
LALRIAEGDVPATLRGATLLGLDLGMLQAGASVKGEFEQRLKRVIAEIRASQTPVVLFIDEAHTLIGAGGAAGASDAANL
LKPALARGELRTIAATTWSEYKKYFEKDAALARRFQPVKLDSPDVATSVMILRGLKERYQDAHGVTIRDDALVAAAELSA
RYITGRQLPDKAIDLLDTACARVKVRQQTKPAALEDAQRAIQALERERRALRDELAERCAPDTPRVADIDRELAALSARA
GALRDAWAAQRDAAQALVDARRACRAAADATAATDAAGTTGTAHAGELADSADVSDIADVAKMADAADAAPADAETGSSA
RDADARMPAGTCARVAPNPDARARPPLRAPHADARERAARALAEATRRFEHAQRDTPLVRIDVDPDAIADVVADWTGIAA
GKLRRDRANVMLRLADTLRRRIRGQDHAIEQISEAVKAGAAGVHDPRRPLGVFLLAGPSGTGKTETALAVADALFGDERS
IVVVNMSEFQERHDVSRLIGSPPGYVGYGEGGMLTEAVRQRPYSVVLLDEVEKAHPDVLNLFYQVFDKGSLSDGEGKEVD
FANTVIFLTSNLGADIIADIAARGARPDPDAMRAAVRPALSRHFKPALLARMTEIPYAPLAPDTLADIARLKLERIAARV
AAQHATRIVYDDAVVAHVAARCTEVESGARNVDFILQRHVLPALAQHVLVCAGNAAPMPAIRVAIDAGGRFVVSNDAPAD
SDARDRSDASDAIDAIDAVDAVDAVDAPNAPNAPNAPNAPNAPNAPNAPNAPNAPNAPNAPNAPNAR*

  Nucleotide sequence: 3084 bp    

>T6CP000599 NC_006349:c756696-753613 [Burkholderia mallei ATCC 23344] [TssH]
ATGCTGCTGGTTGACCTGAAACCGTTGATCGAACAGCTCAACCCCTATTGCCGGAACGCGCTCGAAAGCGCCGTCGGCGC
GTGCGTCGCGCGCCGGCACGACGACGTCGCCGTCGAGCACCTGCTCGCGCGGCTGTGCGACGAACCGTCGGCCGACGTCG
CGCTGTTGCTGCGCGCGTGCGGCGCGGACGCGGCGCGCCTGCGCCGGCAGGCCGACGCCGCGCTCGACGCGCGCCCGGCG
GGCGACGGCGGCCGCCCCGCGTTCGCGCCGTCGCTGCTCGCGCTGTTGCAGGACGCGTGGCTGATCGCGTCGCTCGAGCT
CGGCGACACGCACATCCGCTCGGCGGCCGTGCTCGCCGCCGCGGTCGCCCGCGCGGCGCGGCAGCCGTCGCCCGGCGGCG
ACGACGTGCTGCAGTCGCTGCGCAAGGACGCGCTCGTCGCGCGCTTTGCGAGCGGCGCGTGCGCGCGATCGATCGAATCG
CGCGCGTCCGGCGCGCTCGACGCCGCGCCGGGCGAGGCGAGCGACACGCGCGACGCATCGAGCGCGATCGCCCGCTACTG
CGAGGACTTCACCGCGAAAGCGCGCGCCGGCGGGATCGATCCAGTCTTCGGTCGCGACGCGGAAATCCGCCAGATCGTCG
ACATCCTCGCGCGGCGGCGCAAGAACAATCCGGTGTGCGTCGGCGAGCCGGGCGTGGGCAAGACGGCCGTCGTCGAAGGG
CTCGCGCTGCGGATCGCCGAAGGCGACGTGCCGGCGACGCTGCGCGGCGCGACGCTGCTCGGCCTCGACCTAGGCATGCT
GCAGGCGGGCGCGAGCGTGAAGGGCGAATTCGAGCAGCGGCTCAAGCGCGTGATCGCCGAGATCCGCGCGTCGCAAACGC
CCGTCGTGCTGTTCATCGACGAAGCGCACACGCTGATCGGCGCGGGCGGCGCGGCGGGCGCGTCCGACGCGGCGAACCTG
CTGAAGCCCGCGCTCGCGCGCGGCGAGCTGCGCACGATCGCAGCCACCACGTGGAGCGAATACAAGAAGTATTTCGAGAA
GGACGCGGCGCTCGCGCGGCGCTTCCAGCCGGTGAAGCTCGACAGCCCGGACGTCGCGACGTCGGTGATGATCCTGCGCG
GGCTGAAGGAGCGCTATCAGGACGCGCACGGCGTGACGATCCGCGACGACGCGCTCGTCGCCGCGGCCGAGCTGTCGGCG
CGCTACATCACCGGCCGGCAGTTGCCGGACAAGGCGATCGACCTGCTCGACACCGCGTGCGCGCGCGTGAAGGTGCGCCA
GCAGACCAAGCCCGCCGCGCTGGAGGACGCGCAGCGCGCGATCCAGGCGCTCGAGCGCGAGCGGCGCGCGCTGCGCGACG
AATTGGCCGAGCGCTGCGCGCCGGATACGCCGCGCGTCGCCGACATCGATCGCGAGCTCGCCGCGCTGTCCGCGCGCGCC
GGCGCGCTGCGCGACGCGTGGGCGGCGCAGCGCGACGCCGCGCAGGCGCTCGTCGACGCACGCCGCGCGTGCCGGGCGGC
GGCGGATGCAACGGCTGCAACGGATGCGGCTGGAACGACCGGAACGGCCCATGCCGGCGAGCTCGCCGATAGTGCCGACG
TTTCCGATATCGCCGATGTCGCCAAGATGGCCGATGCGGCCGACGCCGCCCCTGCCGATGCCGAAACCGGCTCGAGCGCC
CGCGACGCCGACGCACGCATGCCCGCCGGGACGTGCGCGCGCGTCGCCCCGAACCCGGATGCGCGGGCGCGGCCTCCTCT
GCGCGCGCCGCACGCCGACGCGCGCGAGCGCGCGGCTCGTGCGCTCGCCGAAGCGACGCGCCGCTTCGAGCACGCGCAGC
GCGACACGCCGCTCGTGCGAATCGACGTCGATCCGGACGCGATCGCCGACGTCGTGGCCGACTGGACCGGCATCGCGGCC
GGCAAGCTGCGCCGCGATCGCGCGAACGTGATGCTGCGGCTCGCCGATACGCTGCGCCGCCGCATCCGCGGCCAGGATCA
CGCGATCGAACAGATCTCCGAAGCCGTGAAGGCGGGCGCGGCCGGCGTCCACGATCCGCGCCGCCCGCTCGGCGTGTTCC
TGCTCGCGGGGCCGTCGGGCACCGGCAAGACCGAGACCGCGCTCGCGGTCGCCGATGCGCTGTTCGGCGACGAGCGCTCG
ATCGTCGTCGTCAACATGAGCGAATTCCAGGAGCGGCACGACGTGAGCCGCCTGATCGGCTCGCCGCCGGGCTACGTCGG
CTACGGCGAGGGCGGGATGCTGACCGAGGCGGTGCGCCAGCGGCCCTATTCGGTCGTGCTGCTAGACGAAGTCGAGAAGG
CGCACCCCGACGTGCTGAACCTGTTCTACCAGGTGTTCGACAAGGGTTCGCTGTCCGACGGCGAAGGCAAGGAGGTCGAC
TTCGCGAACACGGTGATCTTCCTCACGTCGAATCTCGGCGCGGACATCATCGCCGACATCGCCGCGCGCGGCGCGCGGCC
CGATCCGGACGCGATGCGCGCGGCGGTGCGCCCGGCGCTGTCGCGCCATTTCAAGCCGGCGCTGCTCGCGCGGATGACCG
AGATTCCGTATGCGCCGCTCGCGCCCGATACGCTCGCCGACATCGCGCGGCTGAAGCTCGAGCGCATCGCGGCGCGCGTC
GCCGCGCAGCACGCGACGCGCATCGTCTACGACGACGCCGTCGTCGCGCACGTCGCCGCGCGCTGCACCGAAGTCGAATC
GGGCGCGCGCAACGTCGATTTCATCCTGCAGCGCCACGTGCTGCCCGCGCTCGCGCAGCACGTGCTCGTGTGCGCGGGCA
ACGCGGCGCCGATGCCTGCGATTCGCGTCGCCATCGACGCCGGCGGCCGCTTCGTCGTCTCGAACGACGCGCCGGCCGAC
TCCGATGCACGGGACCGGAGCGATGCGTCTGATGCGATCGACGCGATCGACGCGGTCGATGCGGTCGATGCGGTCGATGC
ACCCAACGCACCCAACGCACCCAACGCACCCAACGCACCCAACGCACCCAACGCACCCAACGCACCCAACGCACCCAACG
CACCCAACGCACCCAACGCACCCAACGCACCCAACGCACGCTGA