Detailed information of component protein


Summary

Component ID T6CP008462
Component protein type TssA
T6SS ID (Type) T6SS00070 experimental (Type i1)
Strain Burkholderia mallei ATCC 23344
Replicon chromosome II
Sequence Protein sequence (645 a.a.); Nucleotide sequence (1935 bp)
Reference
[1] Schell MA, Ulrich RL, Ribot WJ, et al. Type VI secretion is a major virulence determinant in Burkholderia mallei. Mol Microbiol. 2007 Jun;64(6):1466-85. PMID: 17555434
[2] Shanks J, Burtnick MN, Brett PJ, et al. Burkholderia mallei tssM encodes a putative deubiquitinase that is secreted and expressed inside infected RAW 264.7 murine macrophages. Infect Immun. 2009 Apr;77(4):1636-48. doi: 10.1128/IAI.01339-08. Epub 2009 Jan 21. PMID: 19168747
[3] Burtnick MN, DeShazer D, Nair V, et al. Burkholderia mallei cluster 1 type VI secretion mutants exhibit growth and actin polymerization defects in RAW 264.7 murine macrophages. Infect Immun. 2010 Jan;78(1):88-99. doi: 10.1128/IAI.00985-09. Epub 2009 Nov 2. PMID: 19884331
[4] Fisher NA, Ribot WJ, Applefeld W, et al. The Madagascar hissing cockroach as a novel surrogate host for Burkholderia pseudomallei, B. mallei and B. thailandensis. BMC Microbiol. 2012 Jun 22;12:117. doi: 10.1186/1471-2180-12-117. PMID: 22892068
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BMA_RS19630
Coordinate (Strand) 765501..767435 (+)
NCBI ID WP_004200978.1
RefSeq NC_006349
Uniprot ID C4B109_BURML, A0A7U8D782_BURML
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS_VasJ PF16989.7 1.2e-83 328..605
ImpA_N PF06812.14 4.3e-28 32..144
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-644 MGMSERRPPGGAAARARMPIDVQALAVLGRTDIDSAMPAGADVRADARFDALHAELAKLASPGASGQVDWRAATHLAAELLRERGKDLLVGCYLAGALLQTGGAAGLRCGLEIVGDLVERHWDAMSPPVSRMRARRGALQWLVDRVDAMHDAGAAACGGACSAELVAQLRAAARRIDALLAERDDDAPTMRAVHAFAERLPVEVVEVVEVADEADVAEAAEAAEAAEAAEAAEAAEAAEAAEAAEAAEAAEAADVADVADVAETAEADAHGSTGGPAAEIAIAAAEQALIDPAGRAAPSAGTDTNANADAARQPARLDEAAGRDRALADALAQLHCVATAFAQADWADARGFRLRRVACWSSVCALPETDAENGRTRIAAPSASIVGAAKNIDEDGEPVAAVRFAEAHAQAFPLWLDLQRIAARALARAGGDGADARREVETAVRALLARLPGLDALTFADGTPFADDATRAWLGELGAPVVAADAVSPSSLPLSPRPSPPERSSPMAGEPARAPGDACGASADDAVDRACAFAASGQLDLALHAIQHAIDRATSAEQRLRARVRLCELARDHWPHEVPEAFARGVIEPIRRHDLLAWNPELALDGLSAAYALLIRRDRESAHARTVLDEIASVDAARAMRLSM


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9182 - -


  Protein sequence: 645 a.a.    

>T6CP008462 NC_006349:765501-767435 [Burkholderia mallei ATCC 23344] [TssA]
MGMSERRPPGGAAARARMPIDVQALAVLGRTDIDSAMPAGADVRADARFDALHAELAKLASPGASGQVDWRAATHLAAEL
LRERGKDLLVGCYLAGALLQTGGAAGLRCGLEIVGDLVERHWDAMSPPVSRMRARRGALQWLVDRVDAMHDAGAAACGGA
CSAELVAQLRAAARRIDALLAERDDDAPTMRAVHAFAERLPVEVVEVVEVADEADVAEAAEAAEAAEAAEAAEAAEAAEA
AEAAEAAEAAEAADVADVADVAETAEADAHGSTGGPAAEIAIAAAEQALIDPAGRAAPSAGTDTNANADAARQPARLDEA
AGRDRALADALAQLHCVATAFAQADWADARGFRLRRVACWSSVCALPETDAENGRTRIAAPSASIVGAAKNIDEDGEPVA
AVRFAEAHAQAFPLWLDLQRIAARALARAGGDGADARREVETAVRALLARLPGLDALTFADGTPFADDATRAWLGELGAP
VVAADAVSPSSLPLSPRPSPPERSSPMAGEPARAPGDACGASADDAVDRACAFAASGQLDLALHAIQHAIDRATSAEQRL
RARVRLCELARDHWPHEVPEAFARGVIEPIRRHDLLAWNPELALDGLSAAYALLIRRDRESAHARTVLDEIASVDAARAM
RLSM*

  Nucleotide sequence: 1935 bp    

>T6CP008462 NC_006349:765501-767435 [Burkholderia mallei ATCC 23344] [TssA]
ATGGGGATGAGCGAACGGCGCCCGCCCGGCGGCGCGGCGGCGCGCGCGCGCATGCCGATCGATGTCCAAGCGCTCGCCGT
GCTGGGGCGCACGGACATCGATTCCGCCATGCCCGCGGGCGCCGACGTGCGCGCCGACGCGAGGTTCGACGCGCTGCACG
CGGAGCTTGCGAAGCTCGCGTCGCCGGGCGCGAGCGGGCAAGTCGATTGGCGCGCGGCGACGCATCTCGCCGCCGAATTG
CTGCGCGAGCGCGGCAAGGATTTGCTCGTCGGCTGCTATCTGGCGGGTGCGTTGCTGCAGACGGGCGGCGCGGCGGGGCT
GCGCTGCGGACTCGAAATCGTCGGCGATCTCGTCGAACGTCATTGGGATGCGATGTCGCCGCCCGTGTCGCGGATGCGGG
CGAGGCGCGGGGCGCTGCAATGGCTGGTCGATCGCGTCGACGCCATGCACGATGCAGGAGCCGCGGCATGCGGCGGCGCG
TGCTCGGCCGAACTGGTCGCGCAATTGCGCGCGGCCGCGCGGCGCATCGATGCGCTGCTCGCCGAGCGTGACGACGACGC
GCCGACGATGCGCGCGGTGCATGCGTTCGCGGAGCGATTGCCTGTTGAGGTGGTTGAGGTGGTGGAAGTGGCTGACGAGG
CTGATGTGGCTGAGGCGGCTGAGGCGGCTGAGGCGGCTGAGGCGGCTGAGGCGGCTGAGGCGGCTGAGGCGGCTGAGGCG
GCTGAGGCGGCTGAGGCGGCTGAGGCGGCTGAGGCGGCTGATGTGGCTGATGTGGCTGATGTGGCTGAGACGGCCGAGGC
CGATGCGCACGGCTCGACGGGAGGGCCGGCCGCGGAAATCGCGATTGCCGCCGCCGAACAGGCTTTGATTGATCCGGCCG
GTCGAGCCGCGCCGAGCGCCGGCACGGATACGAACGCGAACGCAGACGCCGCCAGGCAACCGGCGCGGCTCGACGAAGCG
GCCGGCCGCGACCGCGCGCTCGCCGATGCGCTCGCGCAATTGCATTGCGTCGCGACGGCGTTCGCGCAAGCGGACTGGGC
CGACGCGCGCGGCTTCCGGCTGCGCCGCGTCGCGTGCTGGTCGAGCGTGTGTGCGCTGCCGGAAACGGACGCGGAGAACG
GAAGAACGCGGATCGCCGCGCCGAGCGCTTCGATCGTCGGCGCGGCGAAGAACATCGACGAGGATGGCGAGCCCGTGGCG
GCGGTGCGCTTCGCCGAAGCGCATGCGCAGGCGTTCCCGCTCTGGCTGGATTTGCAGCGCATCGCCGCGCGCGCGCTCGC
GCGCGCGGGAGGCGACGGCGCCGATGCGCGGCGCGAAGTGGAGACGGCGGTTCGTGCACTGCTTGCGCGGCTGCCGGGCC
TCGACGCGCTGACGTTCGCGGACGGCACGCCGTTCGCCGACGACGCGACGCGCGCATGGCTCGGCGAGCTTGGCGCGCCT
GTTGTGGCGGCGGATGCGGTGTCGCCGTCGTCTTTGCCGCTTTCGCCGCGACCTTCGCCGCCTGAGCGATCGTCGCCGAT
GGCGGGCGAACCGGCGCGCGCGCCGGGCGATGCGTGCGGGGCGAGCGCCGACGATGCAGTGGACCGAGCGTGCGCGTTTG
CCGCGAGCGGCCAGCTCGATCTCGCGCTCCACGCGATTCAGCATGCGATCGATCGTGCGACGAGCGCCGAACAGCGGTTG
AGAGCGCGCGTGCGGTTGTGCGAGCTTGCGCGCGACCATTGGCCGCATGAGGTTCCTGAGGCGTTCGCGCGCGGCGTGAT
CGAACCGATTCGGCGGCACGATTTGCTCGCATGGAATCCGGAGCTGGCGCTCGACGGCTTGTCGGCCGCCTATGCGCTGC
TGATTCGGCGCGATCGCGAATCGGCGCACGCGAGGACGGTGCTTGACGAGATCGCGAGCGTCGACGCGGCGCGGGCCATG
CGTTTGTCGATGTGA