Detailed information of component protein


Summary

Component ID T6CP000601
Component protein type TssF
T6SS ID (Type) T6SS00070 experimental (Type i1)
Strain Burkholderia mallei ATCC 23344
Replicon chromosome II
Sequence Protein sequence (581 a.a.); Nucleotide sequence (1743 bp)
Reference
[1] Schell MA, Ulrich RL, Ribot WJ, et al. Type VI secretion is a major virulence determinant in Burkholderia mallei. Mol Microbiol. 2007 Jun;64(6):1466-85. PMID: 17555434
[2] Shanks J, Burtnick MN, Brett PJ, et al. Burkholderia mallei tssM encodes a putative deubiquitinase that is secreted and expressed inside infected RAW 264.7 murine macrophages. Infect Immun. 2009 Apr;77(4):1636-48. doi: 10.1128/IAI.01339-08. Epub 2009 Jan 21. PMID: 19168747
[3] Burtnick MN, DeShazer D, Nair V, et al. Burkholderia mallei cluster 1 type VI secretion mutants exhibit growth and actin polymerization defects in RAW 264.7 murine macrophages. Infect Immun. 2010 Jan;78(1):88-99. doi: 10.1128/IAI.00985-09. Epub 2009 Nov 2. PMID: 19884331
[4] Fisher NA, Ribot WJ, Applefeld W, et al. The Madagascar hissing cockroach as a novel surrogate host for Burkholderia pseudomallei, B. mallei and B. thailandensis. BMC Microbiol. 2012 Jun 22;12:117. doi: 10.1186/1471-2180-12-117. PMID: 22892068
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BMA_RS19595
Coordinate (Strand) 757693..759435 (-)
NCBI ID WP_004200971.1
RefSeq NC_006349
Uniprot ID A5PM46_BURML, A0A0H2XD73_BURMA, B1H8I7_BURPE, A0A6C6YN27_BURM7, C4B100_BURML, A0A7U8D4E1_BURML, A3P6V2_BURP0, A2RXW5_BURM9, A0A0E1UUX4_BURPE
KEGG ID bma:BMAA0740, bml:BMA10229_0719, bmae:DM78_3357, bpl:BURPS1106A_A2029, bmaq:DM76_3654, bmaz:BM44_4693, bmn:BMA10247_A1677, bmal:DM55_4702, bmab:BM45_3165, bmaf:DM51_4440, bmai:DM57_11890
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS_TssF PF05947.14 4.7e-156 7..577
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-580 MATTTPNRYYEDELVRLRELAAEFARAHPLLAPMLGAPSGDPDVERLLEGVAFLTGLARQKLDEGLPELVQALANLLFPHSLRPVPAATLIAFEPRGALRERAVIAAGTEIESVPVDGTACRFRTCGELDIEPIALAGCRFVPPAHGGPALRLDFEMLGLDASEWDATRIRLFIGGERLHASRLFALLMQHVVAVEIAGGPPELPGPRCALGARALRPAGFDDALLPCPERAFPGFRLLHEYFAFAEKFLFVELGGLERWRAARAGAQFSVWLALDSAPDWLPGIDRDSFRLNVAAALNLFAHEAVPIQHEHRATDYRLQPEGDTSGHYRIYSVDRVIGYRPGHAVDRHYVPFGVAGDDANAASYRLIRRAALDGHGQDLHLALAYPPGEALATETLSIGLSCTNGALPARLKIGDVCRATDSSPERFTFANIAPVSPPLDPPLGEPLLWRTISHLALNFLSLGDADHLKRMLALHAFGERGDDARAQADRRRIDGIESVDVRAETRIIGERMLSGQRVALRCSAHAFGGAGELYLFGCVLERFLAEYAAINTYTRVEIDASPDGVRFAWPPRMGAQCLL


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9812 - -


  Protein sequence: 581 a.a.    

>T6CP000601 NC_006349:c759435-757693 [Burkholderia mallei ATCC 23344] [TssF]
MATTTPNRYYEDELVRLRELAAEFARAHPLLAPMLGAPSGDPDVERLLEGVAFLTGLARQKLDEGLPELVQALANLLFPH
SLRPVPAATLIAFEPRGALRERAVIAAGTEIESVPVDGTACRFRTCGELDIEPIALAGCRFVPPAHGGPALRLDFEMLGL
DASEWDATRIRLFIGGERLHASRLFALLMQHVVAVEIAGGPPELPGPRCALGARALRPAGFDDALLPCPERAFPGFRLLH
EYFAFAEKFLFVELGGLERWRAARAGAQFSVWLALDSAPDWLPGIDRDSFRLNVAAALNLFAHEAVPIQHEHRATDYRLQ
PEGDTSGHYRIYSVDRVIGYRPGHAVDRHYVPFGVAGDDANAASYRLIRRAALDGHGQDLHLALAYPPGEALATETLSIG
LSCTNGALPARLKIGDVCRATDSSPERFTFANIAPVSPPLDPPLGEPLLWRTISHLALNFLSLGDADHLKRMLALHAFGE
RGDDARAQADRRRIDGIESVDVRAETRIIGERMLSGQRVALRCSAHAFGGAGELYLFGCVLERFLAEYAAINTYTRVEID
ASPDGVRFAWPPRMGAQCLL*

  Nucleotide sequence: 1743 bp    

>T6CP000601 NC_006349:c759435-757693 [Burkholderia mallei ATCC 23344] [TssF]
ATGGCCACCACCACGCCCAATCGCTATTACGAGGACGAGCTCGTTCGCCTGCGCGAGCTCGCCGCCGAGTTCGCCCGCGC
GCACCCGCTGCTCGCGCCGATGCTCGGCGCGCCGTCGGGCGACCCGGACGTCGAGCGGCTGCTCGAAGGCGTCGCGTTCC
TGACGGGGCTCGCGCGGCAAAAGCTCGACGAAGGCCTGCCGGAGCTCGTGCAGGCGCTCGCGAACCTGCTGTTTCCGCAC
TCGCTGCGCCCGGTGCCGGCGGCCACGCTGATCGCGTTCGAGCCGCGCGGCGCGCTGCGCGAGCGCGCGGTGATCGCGGC
CGGCACCGAGATCGAATCGGTGCCCGTCGACGGCACCGCCTGCCGGTTTCGCACGTGCGGCGAGCTCGACATCGAGCCGA
TCGCGCTCGCCGGCTGCCGGTTCGTGCCGCCCGCGCACGGCGGGCCCGCGCTGCGGCTCGACTTCGAGATGCTCGGCCTC
GACGCGAGCGAATGGGACGCAACGCGCATTCGGCTGTTCATCGGCGGCGAGCGGCTGCACGCAAGCCGCCTGTTCGCGCT
GCTGATGCAGCACGTCGTAGCGGTCGAGATCGCGGGCGGCCCGCCCGAGCTGCCCGGCCCGCGCTGCGCGCTCGGCGCCC
GCGCGCTGCGCCCGGCGGGCTTCGACGACGCGCTGTTGCCCTGCCCCGAGCGGGCGTTCCCCGGCTTCCGGCTGCTGCAC
GAATATTTCGCGTTCGCCGAGAAATTCCTGTTCGTCGAGCTGGGCGGCCTCGAGCGCTGGCGCGCGGCGCGCGCTGGCGC
GCAGTTCAGCGTATGGCTCGCGCTCGACAGCGCGCCCGACTGGCTGCCCGGCATCGATCGCGACAGCTTCCGGCTGAACG
TCGCCGCCGCGCTGAACCTGTTCGCGCACGAAGCGGTGCCGATCCAGCACGAGCATCGCGCGACCGATTACCGGCTGCAG
CCCGAAGGCGACACGTCGGGCCACTACCGGATCTACTCGGTCGACCGCGTGATCGGTTACCGCCCCGGCCACGCGGTCGA
CCGCCATTACGTGCCGTTCGGCGTCGCGGGCGACGACGCGAACGCCGCGAGCTACCGGCTGATTCGCCGCGCCGCGCTCG
ACGGCCACGGGCAGGATCTCCATCTGGCGCTCGCCTACCCGCCCGGCGAGGCGCTCGCCACGGAAACGCTGTCGATCGGC
CTGTCGTGCACGAACGGCGCGTTGCCCGCGCGCCTGAAGATCGGCGACGTGTGCCGCGCGACCGACAGCTCGCCCGAGCG
CTTCACGTTCGCCAACATCGCGCCCGTGAGCCCGCCGCTCGATCCGCCGCTCGGCGAACCGCTGCTGTGGCGCACCATCA
GCCATCTCGCGCTGAACTTCCTGTCGCTCGGCGACGCCGATCATCTGAAGCGCATGCTCGCGCTCCACGCGTTCGGCGAA
CGCGGCGACGACGCGCGCGCGCAAGCCGACCGCCGCCGCATCGACGGCATCGAATCGGTCGACGTGCGGGCCGAGACGCG
GATCATCGGCGAGCGGATGCTGAGCGGCCAGCGCGTGGCGCTGCGCTGCAGCGCCCATGCGTTCGGCGGCGCGGGCGAGC
TGTATCTGTTCGGCTGCGTGCTCGAGCGCTTTCTGGCCGAATACGCGGCGATCAACACCTACACGCGCGTCGAGATCGAC
GCGTCGCCCGACGGCGTGCGCTTCGCGTGGCCGCCGCGAATGGGGGCGCAATGCCTGCTCTAG