Detailed information of component protein


Summary

Component ID T6CP000598
Component protein type TssI
T6SS ID (Type) T6SS00070 experimental (Type i1)
Strain Burkholderia mallei ATCC 23344
Replicon chromosome II
Sequence Protein sequence (1008 a.a.); Nucleotide sequence (3024 bp)
Reference
[1] Schell MA, Ulrich RL, Ribot WJ, et al. Type VI secretion is a major virulence determinant in Burkholderia mallei. Mol Microbiol. 2007 Jun;64(6):1466-85. PMID: 17555434
[2] Shanks J, Burtnick MN, Brett PJ, et al. Burkholderia mallei tssM encodes a putative deubiquitinase that is secreted and expressed inside infected RAW 264.7 murine macrophages. Infect Immun. 2009 Apr;77(4):1636-48. doi: 10.1128/IAI.01339-08. Epub 2009 Jan 21. PMID: 19168747
[3] Burtnick MN, DeShazer D, Nair V, et al. Burkholderia mallei cluster 1 type VI secretion mutants exhibit growth and actin polymerization defects in RAW 264.7 murine macrophages. Infect Immun. 2010 Jan;78(1):88-99. doi: 10.1128/IAI.00985-09. Epub 2009 Nov 2. PMID: 19884331
[4] Fisher NA, Ribot WJ, Applefeld W, et al. The Madagascar hissing cockroach as a novel surrogate host for Burkholderia pseudomallei, B. mallei and B. thailandensis. BMC Microbiol. 2012 Jun 22;12:117. doi: 10.1186/1471-2180-12-117. PMID: 22892068
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BMA_RS19580
Coordinate (Strand) 750563..753586 (-)
NCBI ID WP_004184913.1
RefSeq NC_006349
Uniprot ID A5PM50_BURML, A0A0H2WD12_BURMA, A0A6C6YIE9_BURM7, C4ANV0_BURML, A0A7U8H9I4_BURML
KEGG ID bmal:DM55_4705, bmn:BMA10247_A1680, bmaq:DM76_3657, bmaz:BM44_4696, bmaf:DM51_4437, bmab:BM45_3162, bma:BMAA0737, bmae:DM78_3354, bmai:DM57_11905
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Phage_GPD PF05954.13 2.1e-73 46..349
Phage_base_V PF04717.14 1.7e-06 418..480
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-1007 MPSSPRHDAPASRANAAPSANARRFTFASDAYDPATFDVVDINGRDAISQPYRFEITLVSRQLRIDFAKMLSRGATLAILPPFGEAGTTRYAGVLAEFEQKKRFRDFTVYRATLVPRLWRLSLYKASDVYLNEQTIPDIVKRVLRAASFGSRDFRMRHGGGYRKRSFVCQYDESHLDFVSRWMEKEGLYYYFEHDGRHETLVIVDDRRHQPGPADDLALRYLPATSLDAGIEADRVQAFTCRATPLPREVVLRDFNHRKAELSLEVRERVARDGVGERVSSDEHFHTKDEGQRYAKLRAEALVCEGRRFAGESTAAGLRAGRFFALSGHYREDFDGRYLVTALTHRGSQAHLLFPDLDAPFGATPGEPIYRAEFEAIAADLQYRPPRTTPKPRAAGVVSAIVDGEGGGKLAELDEYGQYKVRFPFAHTAHPANKASARIRMATPYAGDDRGMHLPLLKRTEVKIAFDGGDPDRPVIVGAVPNSSHRSVVTRRNPAEHRILTEHNQLYMKDGSGAATWLHAPNNHIGIGAVGPGDGLALLTSGNKFDFSLGNAYSFSGGLKCSVSMGGNTDVYVGVRNSLDVSANFLTTLQGNLRWMLPGSRSFEINDSASTLLQTLHKQSATGAIRLSAGQDASALLQKQLDKLKGTVRKFMIVSGLANAGSAAAAAGLIKGGGKLADLPWAGFGISAAQFAGVTGVSTALMATSRTLLANVAKLQEALPLVADLSLGKQGIALAAKNLTHATRMSLTVDGVSWSTHAKGPGAAGAAMSVGKGRWGVEAAKHAHVHASDTLLFAVPADPTTQFDLKDLIGLRRDLDECMKDIADLEADISENEVLSTDQNTFGVSALIPTPPSPAGALAAVAIKVKQAKLVELKAKQKLVALKVDNLQQKFAKHVQHLSAVRMSASDAQLGFKGNRLVATADGVTLAHAQGKAKLDVREAKIGVTAGKSSVELDEGKIAAGCGSASLKLGSDGAIDVSATDVKLNGTNVKLNGSASLKFDGQLIQLG


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.8969 - -


  Protein sequence: 1008 a.a.    

>T6CP000598 NC_006349:c753586-750563 [Burkholderia mallei ATCC 23344] [TssI]
MPSSPRHDAPASRANAAPSANARRFTFASDAYDPATFDVVDINGRDAISQPYRFEITLVSRQLRIDFAKMLSRGATLAIL
PPFGEAGTTRYAGVLAEFEQKKRFRDFTVYRATLVPRLWRLSLYKASDVYLNEQTIPDIVKRVLRAASFGSRDFRMRHGG
GYRKRSFVCQYDESHLDFVSRWMEKEGLYYYFEHDGRHETLVIVDDRRHQPGPADDLALRYLPATSLDAGIEADRVQAFT
CRATPLPREVVLRDFNHRKAELSLEVRERVARDGVGERVSSDEHFHTKDEGQRYAKLRAEALVCEGRRFAGESTAAGLRA
GRFFALSGHYREDFDGRYLVTALTHRGSQAHLLFPDLDAPFGATPGEPIYRAEFEAIAADLQYRPPRTTPKPRAAGVVSA
IVDGEGGGKLAELDEYGQYKVRFPFAHTAHPANKASARIRMATPYAGDDRGMHLPLLKRTEVKIAFDGGDPDRPVIVGAV
PNSSHRSVVTRRNPAEHRILTEHNQLYMKDGSGAATWLHAPNNHIGIGAVGPGDGLALLTSGNKFDFSLGNAYSFSGGLK
CSVSMGGNTDVYVGVRNSLDVSANFLTTLQGNLRWMLPGSRSFEINDSASTLLQTLHKQSATGAIRLSAGQDASALLQKQ
LDKLKGTVRKFMIVSGLANAGSAAAAAGLIKGGGKLADLPWAGFGISAAQFAGVTGVSTALMATSRTLLANVAKLQEALP
LVADLSLGKQGIALAAKNLTHATRMSLTVDGVSWSTHAKGPGAAGAAMSVGKGRWGVEAAKHAHVHASDTLLFAVPADPT
TQFDLKDLIGLRRDLDECMKDIADLEADISENEVLSTDQNTFGVSALIPTPPSPAGALAAVAIKVKQAKLVELKAKQKLV
ALKVDNLQQKFAKHVQHLSAVRMSASDAQLGFKGNRLVATADGVTLAHAQGKAKLDVREAKIGVTAGKSSVELDEGKIAA
GCGSASLKLGSDGAIDVSATDVKLNGTNVKLNGSASLKFDGQLIQLG*

  Nucleotide sequence: 3024 bp    

>T6CP000598 NC_006349:c753586-750563 [Burkholderia mallei ATCC 23344] [TssI]
ATGCCTTCGTCCCCCCGCCACGATGCGCCCGCGTCGCGCGCGAACGCCGCGCCGTCCGCGAACGCGCGACGCTTCACGTT
CGCGAGCGACGCGTACGACCCCGCGACATTCGACGTCGTCGACATCAACGGCCGCGACGCGATCTCGCAGCCGTACCGGT
TCGAGATCACGCTCGTGAGCAGGCAGTTGCGGATCGACTTCGCGAAGATGCTGAGCCGCGGGGCGACGCTCGCGATCCTG
CCGCCGTTCGGCGAGGCCGGCACCACCCGCTATGCCGGCGTGCTCGCCGAATTCGAGCAGAAGAAACGCTTTCGCGACTT
CACCGTCTATCGCGCGACGCTCGTGCCGCGCCTCTGGCGACTATCGCTGTACAAGGCGTCGGACGTCTATCTGAACGAGC
AGACGATTCCCGACATCGTCAAGCGCGTGCTGCGCGCCGCCTCGTTCGGCAGCCGCGATTTCCGCATGCGGCACGGCGGC
GGCTACCGCAAGCGCAGCTTCGTCTGCCAATACGACGAGAGCCATCTCGATTTCGTGTCGCGCTGGATGGAGAAGGAAGG
CCTCTACTACTACTTCGAGCATGACGGCCGGCACGAAACGCTCGTGATCGTCGACGACCGCCGCCATCAGCCCGGCCCCG
CCGACGATCTCGCGCTGCGCTACCTACCCGCGACCAGCCTCGACGCGGGCATCGAAGCGGACCGCGTGCAGGCGTTCACA
TGCCGGGCGACGCCGCTGCCGCGCGAAGTCGTGCTGCGCGACTTCAACCACCGCAAGGCGGAGCTCTCGCTCGAAGTCCG
CGAGCGCGTGGCGCGCGACGGCGTCGGCGAGCGGGTGTCGAGCGACGAGCACTTCCACACGAAGGACGAAGGGCAGCGCT
ACGCGAAGCTGCGCGCCGAGGCGCTCGTCTGCGAAGGGCGCCGATTCGCCGGCGAATCGACCGCGGCCGGGCTGCGCGCC
GGCCGCTTCTTCGCGCTGTCGGGCCACTACCGCGAGGATTTCGACGGCCGCTATCTGGTGACGGCGCTCACGCATCGCGG
CTCGCAGGCACACCTGCTGTTTCCCGATCTCGACGCGCCGTTCGGCGCGACGCCGGGCGAGCCCATCTACCGCGCCGAGT
TCGAGGCGATTGCCGCCGACCTCCAGTACCGGCCGCCGCGCACGACGCCCAAGCCGCGCGCGGCGGGCGTCGTCAGCGCG
ATCGTCGACGGCGAGGGCGGCGGCAAGCTCGCCGAGCTCGACGAATACGGCCAGTACAAGGTGCGCTTTCCGTTCGCGCA
CACCGCGCATCCGGCGAACAAGGCCTCCGCGCGCATCCGGATGGCGACGCCCTACGCGGGCGACGACCGCGGCATGCACC
TGCCGCTGCTGAAGCGCACCGAAGTGAAGATCGCATTCGACGGCGGCGATCCGGACCGCCCCGTGATCGTCGGCGCGGTG
CCCAACTCGTCGCACCGCAGCGTCGTCACGCGCCGCAACCCCGCCGAGCATCGCATCCTCACCGAGCACAACCAGCTCTA
CATGAAGGACGGCAGCGGCGCGGCGACGTGGCTGCACGCGCCGAACAACCACATCGGCATCGGCGCGGTCGGGCCGGGCG
ACGGCCTCGCGCTCCTCACGTCCGGCAACAAGTTCGACTTCTCGCTCGGCAACGCGTACAGCTTCTCGGGCGGGCTCAAG
TGCTCGGTGTCGATGGGCGGCAACACCGACGTCTACGTCGGCGTGCGCAACAGCCTCGACGTCAGCGCGAACTTCCTGAC
GACGCTGCAGGGCAACCTGCGCTGGATGCTGCCCGGCAGCCGGAGCTTCGAGATCAACGACAGCGCATCGACACTGCTGC
AGACGCTGCACAAGCAGTCCGCGACGGGCGCGATCCGGCTGTCCGCCGGGCAGGACGCGTCCGCGCTGCTGCAAAAGCAG
CTCGACAAGCTCAAGGGCACGGTGCGCAAGTTCATGATCGTGTCGGGCCTCGCGAACGCCGGGTCCGCGGCCGCCGCCGC
GGGGCTCATCAAGGGCGGCGGCAAGCTCGCCGATCTGCCGTGGGCGGGCTTCGGCATATCCGCCGCGCAGTTCGCCGGCG
TGACCGGCGTCAGCACGGCGCTGATGGCGACCTCGCGCACGCTGCTCGCGAACGTCGCGAAGCTCCAGGAGGCGTTGCCG
CTCGTCGCCGATCTGTCGCTCGGCAAGCAGGGCATCGCGCTCGCCGCGAAGAACCTCACGCACGCGACGCGGATGTCGCT
CACCGTCGACGGCGTCTCGTGGTCGACGCACGCGAAGGGGCCCGGCGCGGCGGGCGCCGCGATGAGCGTCGGCAAGGGCC
GCTGGGGCGTCGAAGCGGCGAAGCACGCGCACGTCCACGCGAGCGACACGCTGCTGTTCGCGGTGCCGGCCGACCCGACG
ACCCAGTTCGACCTGAAGGACCTGATCGGGCTGCGCCGCGATCTCGACGAATGCATGAAGGACATCGCCGATCTCGAAGC
CGACATTTCGGAAAACGAAGTGCTGTCGACCGATCAGAACACGTTCGGCGTCAGCGCGCTCATCCCCACGCCGCCGTCGC
CCGCCGGCGCGCTCGCGGCGGTCGCGATCAAGGTGAAGCAAGCGAAGCTCGTCGAGCTGAAGGCCAAGCAGAAGCTCGTC
GCGCTGAAGGTCGACAACCTGCAGCAGAAGTTCGCGAAGCACGTGCAGCACCTGAGCGCCGTGCGGATGAGCGCTTCCGA
CGCGCAGCTCGGCTTCAAGGGCAACCGGCTCGTCGCGACGGCCGACGGCGTCACGCTCGCGCATGCGCAGGGCAAGGCGA
AGCTCGACGTGCGCGAAGCGAAGATCGGCGTCACGGCGGGCAAATCGAGCGTCGAGCTCGACGAAGGCAAGATCGCGGCC
GGCTGCGGCAGCGCATCGCTGAAGCTCGGCAGCGACGGCGCGATCGACGTGAGCGCGACCGACGTCAAGCTGAACGGCAC
CAACGTCAAGCTGAACGGCAGCGCGTCGCTGAAGTTCGACGGCCAACTGATCCAGCTCGGCTGA